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The map label for this gene is ahpF [H]

Identifier: 66046205

GI number: 66046205

Start: 3566107

End: 3567669

Strand: Reverse

Name: ahpF [H]

Synonym: Psyr_2974

Alternate gene names: 66046205

Gene position: 3567669-3566107 (Counterclockwise)

Preceding gene: 66046206

Following gene: 66046203

Centisome position: 58.55

GC content: 59.88

Gene sequence:

>1563_bases
ATGTTGGACGCCAATCTTAAAGCCCAGTTGAAATCCTATCTGGAGCGGGTTACTCGCCCCATCGAGATCGTCGCGTCCCT
TGATGACGGCGCGAAGTCCCAGGAAATGCTTGCACTGCTCAATGATGTGATCAGCGTTTCCAAGGACGTCACCCTGAATG
ACAGCGGCACCGACGCACGTACTCCGTCGTTCTCGCTGAGCAGCCCTGGCCACGATATCAGCCTGCGTTTTGCTGGTATC
CCCATGGGTCACGAATTCACCTCGCTGGTCCTGGCGCTGCTGCAAGTCGGCGGCTATCCACCCAAGGTCAGTGCGGAAAC
CATCGAACAGGTTCGCGCGCTTGAGGGCGAATTCCATTTCGAAACCTATTTCTCGCAGTCCTGCCAGAACTGCCCGGACG
TTGTCCAGGCACTGAATCTGATGGCAGTACTGAACCCTGGTATCAAGCACGTCGCTATCGACGGTGCGCTGTTCCAGGAC
GAAGTCACCGATCGCAAGATCATGTCGGTACCGAGCATCTATCTGAACGGTGAATTGTTCGGCCAGGGCCGCATGGGCCT
GGAAGAGATTCTGGCCAAAATCGACACCGGTGCCGGTGCGCGTCAGGCGGAAAAACTCAACGCCAAGCAGGCTTTCGATG
TGCTGGTCGTGGGCGGTGGCCCCGCGGGCTCTGCGGCGGCGGTCTACGCTGCCCGCAAAGGCATCCGCACCGGTGTTGCC
GCCGAGCGTTTCGGTGGTCAGGTGCTCGACACCATGGCCATCGAGAACTTCATCTCGGTACAGCATACCGAGGGGCCGAA
ACTGGCCGTGGCGCTTGAAGAACACGTCAAGCAGTACGAAGTCGACATCATGAACCTGCAACGCGCCGACAAGCTGATCC
CTGGCGCAGCCGGCGAGTTGCATGAAGTGAAGTTTGCCAGCGGTGCCAGCCTCAAGGCCAAGACGTTGATCCTTGCCACC
GGCGCACGCTGGAGGGAGATGAATGTGCCGGGTGAGCAGCAGTACCGTAACAAGGGCGTGGCTTATTGCCCGCACTGCGA
CGGTCCGTTGTTCAAAGGCAAGCGCGTCGCTGTCATCGGTGGTGGTAACTCGGGTGTCGAGGCTGCAATCGACCTGGCCG
GTATCGTGTCCCATGTGACACTGCTGGAGTTCGACGTGCAGTTGCGTGCCGACGCGGTGCTGCAGCGCAAGCTGCACAGC
CTGCCGAACGTGACCGTGATCACCAGTGCGCAAACCACTGAAGTGACGGGTGATGAGCAGAAGGTCAACGGCCTGCGCTA
CAAGAACCGCACCACAGGCGAAGAGATCACCGTGCCGCTCGAAGGTATCTTCGTGCAGATCGGTCTGCTGCCCAACAGCG
AATGGCTCAAAGGTGCCATCGAGCTGTCGCCGCGTGGCGAGATCGTGGTCGATTCTCGTGGAGAAACTTCGGTGCCGGGT
ATCTTCGCTGCCGGTGACGTGACAGTCGCCCCGTACAAGCAGATCATCATCGCCCTGGGCGAAGGTGCCAAGGCCTCCCT
GAGCGCCTTTGACCACCTGATCCGTACCTCCGCACCGGCTTGA

Upstream 100 bases:

>100_bases
TGAGCTCTTGAGCAAGTAAACCGCATCGCCCCAAAAAACGCCCGGGCGGTCATCGCCTGGGCGTTGTTATTTCTACCCTT
TGTAAACAGGAAGCCGCCAC

Downstream 100 bases:

>100_bases
TCGGACAGCGGTAAACAAAAAGCCCCGTGAGCGATCACGGGGCTTTTTGTTTTTCAGGCTGATCGCTCCTCGCCGGAGCG
ATCACCGCCAGTCTCAGAGC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MLDANLKAQLKSYLERVTRPIEIVASLDDGAKSQEMLALLNDVISVSKDVTLNDSGTDARTPSFSLSSPGHDISLRFAGI
PMGHEFTSLVLALLQVGGYPPKVSAETIEQVRALEGEFHFETYFSQSCQNCPDVVQALNLMAVLNPGIKHVAIDGALFQD
EVTDRKIMSVPSIYLNGELFGQGRMGLEEILAKIDTGAGARQAEKLNAKQAFDVLVVGGGPAGSAAAVYAARKGIRTGVA
AERFGGQVLDTMAIENFISVQHTEGPKLAVALEEHVKQYEVDIMNLQRADKLIPGAAGELHEVKFASGASLKAKTLILAT
GARWREMNVPGEQQYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHS
LPNVTVITSAQTTEVTGDEQKVNGLRYKNRTTGEEITVPLEGIFVQIGLLPNSEWLKGAIELSPRGEIVVDSRGETSVPG
IFAAGDVTVAPYKQIIIALGEGAKASLSAFDHLIRTSAPA

Sequences:

>Translated_520_residues
MLDANLKAQLKSYLERVTRPIEIVASLDDGAKSQEMLALLNDVISVSKDVTLNDSGTDARTPSFSLSSPGHDISLRFAGI
PMGHEFTSLVLALLQVGGYPPKVSAETIEQVRALEGEFHFETYFSQSCQNCPDVVQALNLMAVLNPGIKHVAIDGALFQD
EVTDRKIMSVPSIYLNGELFGQGRMGLEEILAKIDTGAGARQAEKLNAKQAFDVLVVGGGPAGSAAAVYAARKGIRTGVA
AERFGGQVLDTMAIENFISVQHTEGPKLAVALEEHVKQYEVDIMNLQRADKLIPGAAGELHEVKFASGASLKAKTLILAT
GARWREMNVPGEQQYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHS
LPNVTVITSAQTTEVTGDEQKVNGLRYKNRTTGEEITVPLEGIFVQIGLLPNSEWLKGAIELSPRGEIVVDSRGETSVPG
IFAAGDVTVAPYKQIIIALGEGAKASLSAFDHLIRTSAPA
>Mature_520_residues
MLDANLKAQLKSYLERVTRPIEIVASLDDGAKSQEMLALLNDVISVSKDVTLNDSGTDARTPSFSLSSPGHDISLRFAGI
PMGHEFTSLVLALLQVGGYPPKVSAETIEQVRALEGEFHFETYFSQSCQNCPDVVQALNLMAVLNPGIKHVAIDGALFQD
EVTDRKIMSVPSIYLNGELFGQGRMGLEEILAKIDTGAGARQAEKLNAKQAFDVLVVGGGPAGSAAAVYAARKGIRTGVA
AERFGGQVLDTMAIENFISVQHTEGPKLAVALEEHVKQYEVDIMNLQRADKLIPGAAGELHEVKFASGASLKAKTLILAT
GARWREMNVPGEQQYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHS
LPNVTVITSAQTTEVTGDEQKVNGLRYKNRTTGEEITVPLEGIFVQIGLLPNSEWLKGAIELSPRGEIVVDSRGETSVPG
IFAAGDVTVAPYKQIIIALGEGAKASLSAFDHLIRTSAPA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=64.0926640926641, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=318, Percent_Identity=34.9056603773585, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=33.2268370607029, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=313, Percent_Identity=32.5878594249201, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55485; Mature: 55485

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANLKAQLKSYLERVTRPIEIVASLDDGAKSQEMLALLNDVISVSKDVTLNDSGTDAR
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCC
TPSFSLSSPGHDISLRFAGIPMGHEFTSLVLALLQVGGYPPKVSAETIEQVRALEGEFHF
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEH
ETYFSQSCQNCPDVVQALNLMAVLNPGIKHVAIDGALFQDEVTDRKIMSVPSIYLNGELF
HHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCEEEHHHHCCCHHHCCCEEEECCEEE
GQGRMGLEEILAKIDTGAGARQAEKLNAKQAFDVLVVGGGPAGSAAAVYAARKGIRTGVA
CCCCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCHHCCCH
AERFGGQVLDTMAIENFISVQHTEGPKLAVALEEHVKQYEVDIMNLQRADKLIPGAAGEL
HHHHCCHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HEVKFASGASLKAKTLILATGARWREMNVPGEQQYRNKGVAYCPHCDGPLFKGKRVAVIG
EEEEECCCCCCEEEEEEEECCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEEEEE
GGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHSLPNVTVITSAQTTEVTGDEQ
CCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCHH
KVNGLRYKNRTTGEEITVPLEGIFVQIGLLPNSEWLKGAIELSPRGEIVVDSRGETSVPG
HHCCCEECCCCCCCEEEEEEEEEEEEEEECCCHHHHCEEEEECCCCCEEEECCCCCCCCC
IFAAGDVTVAPYKQIIIALGEGAKASLSAFDHLIRTSAPA
EEEECCEEECCHHEEEEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDANLKAQLKSYLERVTRPIEIVASLDDGAKSQEMLALLNDVISVSKDVTLNDSGTDAR
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCC
TPSFSLSSPGHDISLRFAGIPMGHEFTSLVLALLQVGGYPPKVSAETIEQVRALEGEFHF
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEH
ETYFSQSCQNCPDVVQALNLMAVLNPGIKHVAIDGALFQDEVTDRKIMSVPSIYLNGELF
HHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCEEEHHHHCCCHHHCCCEEEECCEEE
GQGRMGLEEILAKIDTGAGARQAEKLNAKQAFDVLVVGGGPAGSAAAVYAARKGIRTGVA
CCCCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCHHCCCH
AERFGGQVLDTMAIENFISVQHTEGPKLAVALEEHVKQYEVDIMNLQRADKLIPGAAGEL
HHHHCCHHHHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HEVKFASGASLKAKTLILATGARWREMNVPGEQQYRNKGVAYCPHCDGPLFKGKRVAVIG
EEEEECCCCCCEEEEEEEECCCCEEECCCCCHHHHHCCCCEECCCCCCCCCCCCEEEEEE
GGNSGVEAAIDLAGIVSHVTLLEFDVQLRADAVLQRKLHSLPNVTVITSAQTTEVTGDEQ
CCCCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCHH
KVNGLRYKNRTTGEEITVPLEGIFVQIGLLPNSEWLKGAIELSPRGEIVVDSRGETSVPG
HHCCCEECCCCCCCEEEEEEEEEEEEEEECCCHHHHCEEEEECCCCCEEEECCCCCCCCC
IFAAGDVTVAPYKQIIIALGEGAKASLSAFDHLIRTSAPA
EEEECCEEECCHHEEEEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]