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The map label for this gene is nikB [H]

Identifier: 66046190

GI number: 66046190

Start: 3547245

End: 3548195

Strand: Direct

Name: nikB [H]

Synonym: Psyr_2959

Alternate gene names: 66046190

Gene position: 3547245-3548195 (Clockwise)

Preceding gene: 66046189

Following gene: 66046191

Centisome position: 58.21

GC content: 61.93

Gene sequence:

>951_bases
ATGAGCCGCTATATCCTCAGTCGCATCGCGCAAGCGCTGCTGGTACTTTGGGGTGCCTACAGCGTCACCTATTTCATTCT
GTACCTGCTGCCCGGCGACACCCTGTCGATCATGCTCAGCGCCTCGGGTGTCGAAATCGACTCACTGTCCGCGCAGGACC
TCGCCAAGGCCAAGCTCTATTACGGGCTGGACCGGGGCATCTTCGAGCAATACGTCGATTTGCTGTGGGGAGCCATACAC
GGTGACTTCGGCAACTCGCTGACGCTCAATCGCCCGGTATCGGAGTTGCTGATCGAACGTCTGCCGCAAACCCTGAGCCT
CGCGGGGCTGGCCATTGTTCTGTCACTGATCGGCGGGACAGGTCTGGCGTATCTCACTGCCTACGTACGCTGGCAGCCCT
TGAAAGTCGCCTTGTCACGCCTGCCGTCGCTGGGCTTCTCGGTGCCGGTCTTCTGGCTGGGCCTGCTGTTCATTCAACTG
TTCGCGTTCACCCTTGGCTGGTTCCCGGCGACCGGCAATCAGGGTTTCTCAAGCCTGATACTGCCCGCCGTCACGCTGGC
GATCCCCAGCGCTGCGGTGTATGCCCAGGTCCTGCAACGCGGCTTTCAGGGCGTCTGGAAAGAGCCCTACATCATAACCG
CCTACGCCAAGGGCCTTACCCGCGCGCAGGTTCAGGCCCGCCATGCGTTCAAGAACGCTGCCCTGCCGCTGCTGACACTG
ATCGGCCTGCAGGTGGGTAACACGGTATCCGGCGCGGTGCTGGTGGAAACCATTTTCGCCCGCTCCGGGGTCGGTCGACT
TGCCCAGGAAGCCGTGCTGCGCCAGGACATCCCGGTGGTACTTGCCATCGTTGCGGTCTCCGCAGCTGCGTTCGTCGTGG
TCAATCTGATCGTTGACCTGCTGTATCCCGCGCTGGATCCGCGCATCGCCCATACGCCAAAGGTCTCCTGA

Upstream 100 bases:

>100_bases
ACGAGCGGGTCTGGCAAGCCGCCACGGCGAAAAACGTACGCAACTTCCACTGGACGGCGGAAGGCTTTGCGCTGTTCAAC
GACATCGAGTTGGCAACGCC

Downstream 100 bases:

>100_bases
AGCCATGACTATCTTCGACAACCGCCTTGCGCGCCTGGCTCGCCTGGTCGTTTCCAGTGAAACCATTGCGGGTAAAACCG
CACCTGCCCTGCAGTGGCGA

Product: binding-protein dependent transport system inner membrane protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSRYILSRIAQALLVLWGAYSVTYFILYLLPGDTLSIMLSASGVEIDSLSAQDLAKAKLYYGLDRGIFEQYVDLLWGAIH
GDFGNSLTLNRPVSELLIERLPQTLSLAGLAIVLSLIGGTGLAYLTAYVRWQPLKVALSRLPSLGFSVPVFWLGLLFIQL
FAFTLGWFPATGNQGFSSLILPAVTLAIPSAAVYAQVLQRGFQGVWKEPYIITAYAKGLTRAQVQARHAFKNAALPLLTL
IGLQVGNTVSGAVLVETIFARSGVGRLAQEAVLRQDIPVVLAIVAVSAAAFVVVNLIVDLLYPALDPRIAHTPKVS

Sequences:

>Translated_316_residues
MSRYILSRIAQALLVLWGAYSVTYFILYLLPGDTLSIMLSASGVEIDSLSAQDLAKAKLYYGLDRGIFEQYVDLLWGAIH
GDFGNSLTLNRPVSELLIERLPQTLSLAGLAIVLSLIGGTGLAYLTAYVRWQPLKVALSRLPSLGFSVPVFWLGLLFIQL
FAFTLGWFPATGNQGFSSLILPAVTLAIPSAAVYAQVLQRGFQGVWKEPYIITAYAKGLTRAQVQARHAFKNAALPLLTL
IGLQVGNTVSGAVLVETIFARSGVGRLAQEAVLRQDIPVVLAIVAVSAAAFVVVNLIVDLLYPALDPRIAHTPKVS
>Mature_315_residues
SRYILSRIAQALLVLWGAYSVTYFILYLLPGDTLSIMLSASGVEIDSLSAQDLAKAKLYYGLDRGIFEQYVDLLWGAIHG
DFGNSLTLNRPVSELLIERLPQTLSLAGLAIVLSLIGGTGLAYLTAYVRWQPLKVALSRLPSLGFSVPVFWLGLLFIQLF
AFTLGWFPATGNQGFSSLILPAVTLAIPSAAVYAQVLQRGFQGVWKEPYIITAYAKGLTRAQVQARHAFKNAALPLLTLI
GLQVGNTVSGAVLVETIFARSGVGRLAQEAVLRQDIPVVLAIVAVSAAAFVVVNLIVDLLYPALDPRIAHTPKVS

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=309, Percent_Identity=36.8932038834951, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1789965, Length=342, Percent_Identity=33.9181286549708, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787053, Length=313, Percent_Identity=33.2268370607029, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1787497, Length=314, Percent_Identity=28.6624203821656, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787550, Length=262, Percent_Identity=29.0076335877863, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788504, Length=363, Percent_Identity=24.5179063360882, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787761, Length=334, Percent_Identity=32.6347305389222, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34099; Mature: 33967

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYILSRIAQALLVLWGAYSVTYFILYLLPGDTLSIMLSASGVEIDSLSAQDLAKAKLY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
YGLDRGIFEQYVDLLWGAIHGDFGNSLTLNRPVSELLIERLPQTLSLAGLAIVLSLIGGT
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
GLAYLTAYVRWQPLKVALSRLPSLGFSVPVFWLGLLFIQLFAFTLGWFPATGNQGFSSLI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LPAVTLAIPSAAVYAQVLQRGFQGVWKEPYIITAYAKGLTRAQVQARHAFKNAALPLLTL
HHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLQVGNTVSGAVLVETIFARSGVGRLAQEAVLRQDIPVVLAIVAVSAAAFVVVNLIVDL
HHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LYPALDPRIAHTPKVS
HHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRYILSRIAQALLVLWGAYSVTYFILYLLPGDTLSIMLSASGVEIDSLSAQDLAKAKLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH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HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]