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The map label for this gene is gfa [H]

Identifier: 66046188

GI number: 66046188

Start: 3544623

End: 3545243

Strand: Direct

Name: gfa [H]

Synonym: Psyr_2957

Alternate gene names: 66046188

Gene position: 3544623-3545243 (Clockwise)

Preceding gene: 66046187

Following gene: 66046189

Centisome position: 58.17

GC content: 55.56

Gene sequence:

>621_bases
ATGGAACCTGTTCCAAGTCACCCCTATAGCTGGAGGTACACTGTGAGCGATGTAAAACTTCACCCTGCGCTGGATAACGG
CATCAAATCCGCTGCTACCGACTTTGCAGGCGGCACTCTGCAGTGCCTTTGCCCGACCGACAAGGTTGAGGTCAAAGTCG
ATGCTCAAACACTGCATAACCACGCCTGCGGTTGCAGTAAATGCTGGAAACCAGCGGGCGCCAGGTTTGCCGTCATCGCG
GTAGTCAATCGCGACAAAGTCAGCGTTACCGTCAACGCAGCCAAACTGACCATCATTGATGCAAGCGCTACCATCCAGCG
TCACGCCTGCAAAGAATGTGGTGCCCATATGTATGGTCGCATCGAAAACCAGAACCACGCGTTCCACGGTCTGGATTTCG
TGCACACCGAACTGTCTCCTCAGACAGGCTGGGCTGCGCCCGGTTTTGCCGCGTTCGTCTCGTCGGTCATTGAAACGGGT
ACGCCGCCAGCTGAAATGGACGGCATTCGCGAACGTTTCAACACGATGGGTTTGCCAACCTACGACTGTCTTTCCCCGGC
ACTGATGGATGCCTTGGCAGCACACGTAGCGAAGCAAAATGGCGTTCTTCAGGCGAGCTGA

Upstream 100 bases:

>100_bases
CTGGATCGCATCAATGAAGCGTTTGACTTGATGCACGAAGGCAAGTCGATCCGCACCGTCATCAAATACTGATGTCGTCC
ACTGTTCCGGGGCAGTTGCC

Downstream 100 bases:

>100_bases
GATAATCCCTGGCGGGCATCACCCACCTTACCAAGCGTGGTGATGGCTTATCCGGAACGCATGCACTGAACCATACAGCA
CTCACAAAGCCCTCCTGACT

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MEPVPSHPYSWRYTVSDVKLHPALDNGIKSAATDFAGGTLQCLCPTDKVEVKVDAQTLHNHACGCSKCWKPAGARFAVIA
VVNRDKVSVTVNAAKLTIIDASATIQRHACKECGAHMYGRIENQNHAFHGLDFVHTELSPQTGWAAPGFAAFVSSVIETG
TPPAEMDGIRERFNTMGLPTYDCLSPALMDALAAHVAKQNGVLQAS

Sequences:

>Translated_206_residues
MEPVPSHPYSWRYTVSDVKLHPALDNGIKSAATDFAGGTLQCLCPTDKVEVKVDAQTLHNHACGCSKCWKPAGARFAVIA
VVNRDKVSVTVNAAKLTIIDASATIQRHACKECGAHMYGRIENQNHAFHGLDFVHTELSPQTGWAAPGFAAFVSSVIETG
TPPAEMDGIRERFNTMGLPTYDCLSPALMDALAAHVAKQNGVLQAS
>Mature_206_residues
MEPVPSHPYSWRYTVSDVKLHPALDNGIKSAATDFAGGTLQCLCPTDKVEVKVDAQTLHNHACGCSKCWKPAGARFAVIA
VVNRDKVSVTVNAAKLTIIDASATIQRHACKECGAHMYGRIENQNHAFHGLDFVHTELSPQTGWAAPGFAAFVSSVIETG
TPPAEMDGIRERFNTMGLPTYDCLSPALMDALAAHVAKQNGVLQAS

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: COG3791

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 22072; Mature: 22072

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVPSHPYSWRYTVSDVKLHPALDNGIKSAATDFAGGTLQCLCPTDKVEVKVDAQTLHN
CCCCCCCCCCEEEEEECEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEECHHHHHC
HACGCSKCWKPAGARFAVIAVVNRDKVSVTVNAAKLTIIDASATIQRHACKECGAHMYGR
CCCCCHHHCCCCCCCEEEEEEEECCEEEEEEEEEEEEEEECCHHHHHHHHHHHCHHHHEE
IENQNHAFHGLDFVHTELSPQTGWAAPGFAAFVSSVIETGTPPAEMDGIRERFNTMGLPT
ECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
YDCLSPALMDALAAHVAKQNGVLQAS
HHHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MEPVPSHPYSWRYTVSDVKLHPALDNGIKSAATDFAGGTLQCLCPTDKVEVKVDAQTLHN
CCCCCCCCCCEEEEEECEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEECHHHHHC
HACGCSKCWKPAGARFAVIAVVNRDKVSVTVNAAKLTIIDASATIQRHACKECGAHMYGR
CCCCCHHHCCCCCCCEEEEEEEECCEEEEEEEEEEEEEEECCHHHHHHHHHHHCHHHHEE
IENQNHAFHGLDFVHTELSPQTGWAAPGFAAFVSSVIETGTPPAEMDGIRERFNTMGLPT
ECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
YDCLSPALMDALAAHVAKQNGVLQAS
HHHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]