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The map label for this gene is nemR [H]

Identifier: 66046164

GI number: 66046164

Start: 3521458

End: 3522039

Strand: Direct

Name: nemR [H]

Synonym: Psyr_2933

Alternate gene names: 66046164

Gene position: 3521458-3522039 (Clockwise)

Preceding gene: 66046160

Following gene: 66046165

Centisome position: 57.79

GC content: 55.33

Gene sequence:

>582_bases
ATGAAGAAACCAGCTCAGGACATGCGACAGCACATCATTGATGTGGCCCGCTCGCTCATGACGAACAAAGGCTATACCGC
CGTCGGCCTCGCAGAGGTGTTAAGCACGGCTGGTGTACCCAAAGGTTCGTTCTATTACTACTTCAAATCGAAGGAAGAAT
TCGGTCAGGCGCTGCTGGAAGAGTATTTTTCCGAGTACCTGGGTCGAGTCGACGCCCTCATGGCACGCCCCGGAAGCGGC
GCCGAGCGGTTACTGGCCTACTTCAACTATTGGATAGAAACCCAGGGCACCGACTTTCCCGAAGGCAAGTGCCTGGTCGT
CAAGCTGGGGGCGGAAGTCTGCGATTTGTCTGAAGACATGCGTGGCGTGCTCGAGGTCGGCACAGCAAAAATCATCAAAC
GCATCACTGCCTGTGTCGACATGGGTGTCGCCGACGACAGCATTCATCCCGAGGGCGATCATGAAGGATTCGCAGAATCC
CTCTATCAGCTCTGGCTTGGCGCTTCCCTTCTGGCGAAGGTGAACAAGTCCACCGAATCTTTCGAAAAAGCACTGACCAT
GACCAAACGCCTGCTGCGGTAG

Upstream 100 bases:

>100_bases
AAAGCCTATGCGGTATACATTTGCTGAGGTTTTTTTGCGCAAGCATATCCAGAAAACTTACTAGACGACTGGTCGGTTTA
CGTTTAGGATGCTCGGCATT

Downstream 100 bases:

>100_bases
CGGGCATTTTTTTTTGTCTTTTCAAGTAGACGACTGGTCTACTATATGGAGTATTTGATGCATGATCCCCGTACAACTAC
CGACCTGTTTTCCCCCGTCA

Product: regulatory protein, TetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKKPAQDMRQHIIDVARSLMTNKGYTAVGLAEVLSTAGVPKGSFYYYFKSKEEFGQALLEEYFSEYLGRVDALMARPGSG
AERLLAYFNYWIETQGTDFPEGKCLVVKLGAEVCDLSEDMRGVLEVGTAKIIKRITACVDMGVADDSIHPEGDHEGFAES
LYQLWLGASLLAKVNKSTESFEKALTMTKRLLR

Sequences:

>Translated_193_residues
MKKPAQDMRQHIIDVARSLMTNKGYTAVGLAEVLSTAGVPKGSFYYYFKSKEEFGQALLEEYFSEYLGRVDALMARPGSG
AERLLAYFNYWIETQGTDFPEGKCLVVKLGAEVCDLSEDMRGVLEVGTAKIIKRITACVDMGVADDSIHPEGDHEGFAES
LYQLWLGASLLAKVNKSTESFEKALTMTKRLLR
>Mature_193_residues
MKKPAQDMRQHIIDVARSLMTNKGYTAVGLAEVLSTAGVPKGSFYYYFKSKEEFGQALLEEYFSEYLGRVDALMARPGSG
AERLLAYFNYWIETQGTDFPEGKCLVVKLGAEVCDLSEDMRGVLEVGTAKIIKRITACVDMGVADDSIHPEGDHEGFAES
LYQLWLGASLLAKVNKSTESFEKALTMTKRLLR

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=192, Percent_Identity=39.0625, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 21453; Mature: 21453

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPAQDMRQHIIDVARSLMTNKGYTAVGLAEVLSTAGVPKGSFYYYFKSKEEFGQALLE
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
EYFSEYLGRVDALMARPGSGAERLLAYFNYWIETQGTDFPEGKCLVVKLGAEVCDLSEDM
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHH
RGVLEVGTAKIIKRITACVDMGVADDSIHPEGDHEGFAESLYQLWLGASLLAKVNKSTES
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
FEKALTMTKRLLR
HHHHHHHHHHHHC
>Mature Secondary Structure
MKKPAQDMRQHIIDVARSLMTNKGYTAVGLAEVLSTAGVPKGSFYYYFKSKEEFGQALLE
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHH
EYFSEYLGRVDALMARPGSGAERLLAYFNYWIETQGTDFPEGKCLVVKLGAEVCDLSEDM
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHH
RGVLEVGTAKIIKRITACVDMGVADDSIHPEGDHEGFAESLYQLWLGASLLAKVNKSTES
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
FEKALTMTKRLLR
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]