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The map label for this gene is nemA [H]

Identifier: 66046165

GI number: 66046165

Start: 3522097

End: 3523200

Strand: Direct

Name: nemA [H]

Synonym: Psyr_2934

Alternate gene names: 66046165

Gene position: 3522097-3523200 (Clockwise)

Preceding gene: 66046164

Following gene: 66046166

Centisome position: 57.8

GC content: 59.96

Gene sequence:

>1104_bases
ATGCATGATCCCCGTACAACTACCGACCTGTTTTCCCCCGTCACCCTGGGCTCCATGGAGCTGGCGAACCGTATTGTGAT
GGCACCGGTAACACGCAGCCGCTATGGCGAGGATGGTGTTCCCAATGAACTGCACGCAACTTACTACGCACAACGCGCTA
CGGCAGGGCTGATCGTCGCCGAGGCGACAAACATTTCCGCTCAGGGTCGAGGCTATGCCGCGACCCCGGGAATCTGGAGT
GAGCAACAGGTCGCAGGTTGGCGCCTGGTGACCGACGCTGTGCATGCAGCAGGAGGCAAGATTGTCAGCCAGCTATGGCA
CGTGGGGCGATTTTCCAGTGTCGACCTGCAACCCAACGGCGAGGCACCGGTAGCGCCCTCCGCCATCAAGGCCGAGGGCA
ATACCTACACCGCCGGCGGTTTTGTACCTGTCTCCATGCCGCGCGCACTGGAAACCGGCGAAATCCCCGGCATCATCGAG
CAATACAAACACGCCGCAGAGAACGCCAAAAGGGCCGGTTTTGATGGCATTGAAGTCCATTCGGCCAACAGTTACCTGCT
GGACCAGTTTCTGCGCGACTCGACCAATCAGCGCACCGACCAGTATGGCGGTTCGATAGAAAACCGCGCTCGCCTGACGC
TGGAAGTCACCCAGGCCATCGTGGATATCTGGGGCAGTGACCGCGTGGGTATTCGCTTGTCGCCCGTGACCCCCGACGCC
GGTAATACGGCACCCGACAGCAACGTCATGGGCCTTCATGGCTACCTGATCCAGCAACTGAACACATTCAATCTGGCCTA
TCTGCACTTTGTCGAAGGCGCCACTGCCACCTCCCGTGAAGTGCCGGAGGGTGTCGACATGGATGCCCTGAGCGCCGGGT
TCAACGGTCCGTTCATTGGCAATAACAACTATGACCTGAAGATGGCGATCGAGCGTCGGGCGCAGGGCAAGATCGACGCC
GTGGCTTTCGGCCGCTTGTTCATATCCAACCCCGACCTCGTCGCCCGTCTGCGCCATGGTGCCGAGCTGACCATTGCACC
ACGTGAGTCGTACTACGGCGGCGGTGCCAAAGGCTATATCGACTGGCCAATGGGCAAGTACTAA

Upstream 100 bases:

>100_bases
TTTCGAAAAAGCACTGACCATGACCAAACGCCTGCTGCGGTAGCGGGCATTTTTTTTTGTCTTTTCAAGTAGACGACTGG
TCTACTATATGGAGTATTTG

Downstream 100 bases:

>100_bases
GCCACTGCCGGCCTCCTCCCCTTCATCAATTCCCAGGATCCCGGAAATACCGCGACAAAGGAAAAACACCACATGAATTA
TCTCGACAAGAAAACCGTAA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MHDPRTTTDLFSPVTLGSMELANRIVMAPVTRSRYGEDGVPNELHATYYAQRATAGLIVAEATNISAQGRGYAATPGIWS
EQQVAGWRLVTDAVHAAGGKIVSQLWHVGRFSSVDLQPNGEAPVAPSAIKAEGNTYTAGGFVPVSMPRALETGEIPGIIE
QYKHAAENAKRAGFDGIEVHSANSYLLDQFLRDSTNQRTDQYGGSIENRARLTLEVTQAIVDIWGSDRVGIRLSPVTPDA
GNTAPDSNVMGLHGYLIQQLNTFNLAYLHFVEGATATSREVPEGVDMDALSAGFNGPFIGNNNYDLKMAIERRAQGKIDA
VAFGRLFISNPDLVARLRHGAELTIAPRESYYGGGAKGYIDWPMGKY

Sequences:

>Translated_367_residues
MHDPRTTTDLFSPVTLGSMELANRIVMAPVTRSRYGEDGVPNELHATYYAQRATAGLIVAEATNISAQGRGYAATPGIWS
EQQVAGWRLVTDAVHAAGGKIVSQLWHVGRFSSVDLQPNGEAPVAPSAIKAEGNTYTAGGFVPVSMPRALETGEIPGIIE
QYKHAAENAKRAGFDGIEVHSANSYLLDQFLRDSTNQRTDQYGGSIENRARLTLEVTQAIVDIWGSDRVGIRLSPVTPDA
GNTAPDSNVMGLHGYLIQQLNTFNLAYLHFVEGATATSREVPEGVDMDALSAGFNGPFIGNNNYDLKMAIERRAQGKIDA
VAFGRLFISNPDLVARLRHGAELTIAPRESYYGGGAKGYIDWPMGKY
>Mature_367_residues
MHDPRTTTDLFSPVTLGSMELANRIVMAPVTRSRYGEDGVPNELHATYYAQRATAGLIVAEATNISAQGRGYAATPGIWS
EQQVAGWRLVTDAVHAAGGKIVSQLWHVGRFSSVDLQPNGEAPVAPSAIKAEGNTYTAGGFVPVSMPRALETGEIPGIIE
QYKHAAENAKRAGFDGIEVHSANSYLLDQFLRDSTNQRTDQYGGSIENRARLTLEVTQAIVDIWGSDRVGIRLSPVTPDA
GNTAPDSNVMGLHGYLIQQLNTFNLAYLHFVEGATATSREVPEGVDMDALSAGFNGPFIGNNNYDLKMAIERRAQGKIDA
VAFGRLFISNPDLVARLRHGAELTIAPRESYYGGGAKGYIDWPMGKY

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=50.1369863013699, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1789463, Length=356, Percent_Identity=30.3370786516854, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17559802, Length=306, Percent_Identity=30.0653594771242, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=198, Percent_Identity=32.8282828282828, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=234, Percent_Identity=29.4871794871795, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=294, Percent_Identity=26.1904761904762, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=208, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565138, Length=124, Percent_Identity=37.0967741935484, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=374, Percent_Identity=38.2352941176471, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=374, Percent_Identity=36.6310160427808, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39612; Mature: 39612

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDPRTTTDLFSPVTLGSMELANRIVMAPVTRSRYGEDGVPNELHATYYAQRATAGLIVA
CCCCCCCHHHCCCCCCCHHHHHCEEEECCCHHHCCCCCCCCCHHHHHHHHHHHCCEEEEE
EATNISAQGRGYAATPGIWSEQQVAGWRLVTDAVHAAGGKIVSQLWHVGRFSSVDLQPNG
EECCCCCCCCCEECCCCCCCCCHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCC
EAPVAPSAIKAEGNTYTAGGFVPVSMPRALETGEIPGIIEQYKHAAENAKRAGFDGIEVH
CCCCCCCEEECCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
SANSYLLDQFLRDSTNQRTDQYGGSIENRARLTLEVTQAIVDIWGSDRVGIRLSPVTPDA
CCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEECCCCCC
GNTAPDSNVMGLHGYLIQQLNTFNLAYLHFVEGATATSREVPEGVDMDALSAGFNGPFIG
CCCCCCCCEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCEEC
NNNYDLKMAIERRAQGKIDAVAFGRLFISNPDLVARLRHGAELTIAPRESYYGGGAKGYI
CCCCEEEEEEEHHCCCCEEEEEEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCEEE
DWPMGKY
CCCCCCC
>Mature Secondary Structure
MHDPRTTTDLFSPVTLGSMELANRIVMAPVTRSRYGEDGVPNELHATYYAQRATAGLIVA
CCCCCCCHHHCCCCCCCHHHHHCEEEECCCHHHCCCCCCCCCHHHHHHHHHHHCCEEEEE
EATNISAQGRGYAATPGIWSEQQVAGWRLVTDAVHAAGGKIVSQLWHVGRFSSVDLQPNG
EECCCCCCCCCEECCCCCCCCCHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCC
EAPVAPSAIKAEGNTYTAGGFVPVSMPRALETGEIPGIIEQYKHAAENAKRAGFDGIEVH
CCCCCCCEEECCCCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
SANSYLLDQFLRDSTNQRTDQYGGSIENRARLTLEVTQAIVDIWGSDRVGIRLSPVTPDA
CCCHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEECCCCCC
GNTAPDSNVMGLHGYLIQQLNTFNLAYLHFVEGATATSREVPEGVDMDALSAGFNGPFIG
CCCCCCCCEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCEEC
NNNYDLKMAIERRAQGKIDAVAFGRLFISNPDLVARLRHGAELTIAPRESYYGGGAKGYI
CCCCEEEEEEEHHCCCCEEEEEEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCEEE
DWPMGKY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]