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The map label for this gene is gcvA [H]

Identifier: 66045767

GI number: 66045767

Start: 2943101

End: 2944012

Strand: Reverse

Name: gcvA [H]

Synonym: Psyr_2531

Alternate gene names: 66045767

Gene position: 2944012-2943101 (Counterclockwise)

Preceding gene: 66045768

Following gene: 66045766

Centisome position: 48.31

GC content: 64.36

Gene sequence:

>912_bases
GTGCGTATCGAGCCTCTTCCGCCGCTGCAGAACCTGATCGCTTTCGAAGCTGCCGTCCGGCATGGCAGCTTCACGCGTGC
CGCCAGCGAACTGAACCTGACCCAGAGCGCGATCAGCCGTCAGGTCGCGCAACTGGAGGAATTTCTCGGCCGCGCGCTGT
TTCGTCGCGAGCACAAGCGCATTCATCTGACGGTCGCCGGGCAGATTTACGCCGAGCAGATCCAGCGCGTATTGACGTTG
TGCGCCGAGGCCACCGCTCAGGTGATCACTCACAGCGGCGATCAGCAGCTGACCCTGGCCTGCTCCAGTGGCGTGGCGGC
CTTGTGGCTGGGGCCTTTGCTGGGACGTTATCGCGATGCCTTCCCGGCCGTGGATACCCGCCTGCGCGTGGTCGACGGGC
TGGATTCGCTGATTCGCGCCGAATTCGATCTGGCGCTGTATTTTCTCCGCGAAGTCTCACCGGCGGGTCTCGACAGCCGC
CTGTTGTTCGCCGAATCGGTCAATGCCTACTGCTCGCCCGACTATCTGGACGGACGTCTGCTGGAGCCCGTGCAGTTGCT
GGATCATTGCCTGCTGATGCTCGAAGACGGCCAGCGTCAATGGTTGTCATGGGGTGACTGGTTCGGCCGCCAGGGTGTCG
ATGCCTCGCTGATCCGTAAGCGCATGACCGTCAATCTGTATCCGGTGCTGGTCGATCTGGCGGTCGCCGGGCACGGCATC
GTGCTCGGCTGGCAGCCAATGATCGACCGCCACGTACAGTCCGGCGCGCTGGTGCCCGCCTGCCGCAAAAGCATCGGCGC
GGTGGGTGGCTACTACCTGCTGACACCCAGCGGCAAGACGCCTCGGCGTGCCGCACGCGCCTTCGAGAAATGGCTGGCGG
ACGAAATCGCCACACTGGCGCCAGTGCCCTGA

Upstream 100 bases:

>100_bases
GCGTTTTCAGAACCTGCCATCGCTGTGCGTGTTCAGGGACTGGCTGCTGTCGACGCCGTTTCCCGACACTTTGTAAAACC
TGCCCGCAACAGGAGCCACT

Downstream 100 bases:

>100_bases
AACCTGCATGCGTCTGGCGCATGCAAATCTGCGTAATAAGCGTTTTCTTAAAATCCGCCCAGCGCCTACTCTCGACTGAA
ACGGGCAAAAAGACCTGACT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRIEPLPPLQNLIAFEAAVRHGSFTRAASELNLTQSAISRQVAQLEEFLGRALFRREHKRIHLTVAGQIYAEQIQRVLTL
CAEATAQVITHSGDQQLTLACSSGVAALWLGPLLGRYRDAFPAVDTRLRVVDGLDSLIRAEFDLALYFLREVSPAGLDSR
LLFAESVNAYCSPDYLDGRLLEPVQLLDHCLLMLEDGQRQWLSWGDWFGRQGVDASLIRKRMTVNLYPVLVDLAVAGHGI
VLGWQPMIDRHVQSGALVPACRKSIGAVGGYYLLTPSGKTPRRAARAFEKWLADEIATLAPVP

Sequences:

>Translated_303_residues
MRIEPLPPLQNLIAFEAAVRHGSFTRAASELNLTQSAISRQVAQLEEFLGRALFRREHKRIHLTVAGQIYAEQIQRVLTL
CAEATAQVITHSGDQQLTLACSSGVAALWLGPLLGRYRDAFPAVDTRLRVVDGLDSLIRAEFDLALYFLREVSPAGLDSR
LLFAESVNAYCSPDYLDGRLLEPVQLLDHCLLMLEDGQRQWLSWGDWFGRQGVDASLIRKRMTVNLYPVLVDLAVAGHGI
VLGWQPMIDRHVQSGALVPACRKSIGAVGGYYLLTPSGKTPRRAARAFEKWLADEIATLAPVP
>Mature_303_residues
MRIEPLPPLQNLIAFEAAVRHGSFTRAASELNLTQSAISRQVAQLEEFLGRALFRREHKRIHLTVAGQIYAEQIQRVLTL
CAEATAQVITHSGDQQLTLACSSGVAALWLGPLLGRYRDAFPAVDTRLRVVDGLDSLIRAEFDLALYFLREVSPAGLDSR
LLFAESVNAYCSPDYLDGRLLEPVQLLDHCLLMLEDGQRQWLSWGDWFGRQGVDASLIRKRMTVNLYPVLVDLAVAGHGI
VLGWQPMIDRHVQSGALVPACRKSIGAVGGYYLLTPSGKTPRRAARAFEKWLADEIATLAPVP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=31.4878892733564, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786448, Length=305, Percent_Identity=31.1475409836066, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=30.1948051948052, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786401, Length=255, Percent_Identity=27.843137254902, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=159, Percent_Identity=33.9622641509434, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=24.9158249158249, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=164, Percent_Identity=29.8780487804878, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789639, Length=141, Percent_Identity=31.2056737588652, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33537; Mature: 33537

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEPLPPLQNLIAFEAAVRHGSFTRAASELNLTQSAISRQVAQLEEFLGRALFRREHKR
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
IHLTVAGQIYAEQIQRVLTLCAEATAQVITHSGDQQLTLACSSGVAALWLGPLLGRYRDA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
FPAVDTRLRVVDGLDSLIRAEFDLALYFLREVSPAGLDSRLLFAESVNAYCSPDYLDGRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
LEPVQLLDHCLLMLEDGQRQWLSWGDWFGRQGVDASLIRKRMTVNLYPVLVDLAVAGHGI
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
VLGWQPMIDRHVQSGALVPACRKSIGAVGGYYLLTPSGKTPRRAARAFEKWLADEIATLA
EECCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
PVP
CCC
>Mature Secondary Structure
MRIEPLPPLQNLIAFEAAVRHGSFTRAASELNLTQSAISRQVAQLEEFLGRALFRREHKR
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
IHLTVAGQIYAEQIQRVLTLCAEATAQVITHSGDQQLTLACSSGVAALWLGPLLGRYRDA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
FPAVDTRLRVVDGLDSLIRAEFDLALYFLREVSPAGLDSRLLFAESVNAYCSPDYLDGRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
LEPVQLLDHCLLMLEDGQRQWLSWGDWFGRQGVDASLIRKRMTVNLYPVLVDLAVAGHGI
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
VLGWQPMIDRHVQSGALVPACRKSIGAVGGYYLLTPSGKTPRRAARAFEKWLADEIATLA
EECCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
PVP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]