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The map label for this gene is citA [H]

Identifier: 66045766

GI number: 66045766

Start: 2941602

End: 2942912

Strand: Reverse

Name: citA [H]

Synonym: Psyr_2530

Alternate gene names: 66045766

Gene position: 2942912-2941602 (Counterclockwise)

Preceding gene: 66045767

Following gene: 66045765

Centisome position: 48.29

GC content: 61.86

Gene sequence:

>1311_bases
GTGAGCACATTACAATCCTCTGCTTCACGCCCCCATGAACAAAAGGCACGCCGCGCGGTAGTCGCCACGGTGATCGGCAA
TGGCCTTGAATGGTTTGACTTCACGGTCTACAGCTTCTTCTCGGTGATCATCGCCAAGGTGTTCTTCCCCACCGGCAGCG
AACTGACGTCTTACCTGCTGGCGCTGGCGACCTTCGGGGTCGGCTTTTTCATGCGTCCGGTGGGCGGCATTGTGCTGGGC
ATCTATAGCGACAAGCGCGGACGCAAGGCGGCATTGTCGCTGACCATCCTGCTGATGGCGCTGGGTACGCTGATCATCGG
GCTTACACCCGGATTCGCGCAGATCGGCTATCTGGCACCGCTGCTGATTGTCCTGGCGCGCCTGCTGCAAGGCTTCTCTG
CCGGTGGCGAGATGGGCAGCGCCACAGCGTTTCTGACCGAGCATGCGCCTGAGGGCAGGAAAGCCTTCTACTCCAGCTGG
ATTCAGGCCAGCATCGGCGTCGCGGTGCTGCTGGGTTCCGCGCTGGGCGCGGTGCTGTCCAGTTACCTGACCCAGGCACA
GCTCGAAAGCTGGGGCTGGAGGGTGCCGTTTCTGATCGGCACGCTGATCGGGCCGGTCGGCTTTTATATTCGCAGCCGGG
TTGACGAGACACCTGCCTACAAGGCTGCCAAACCGGTCGAATCGCCGCTGCGCGAAGTGATCAAGACCTACCCGCGACAG
ACCCTGATCAGCTTTTCGCTGGTCGTGCTGTGGACCGTCTGCACCTATGCCATCCTGTTTTACATTCCGTCCTACGCCCA
GCGCGTGCTTGGTCTGCCGTCATGGATGGGCTTCAGCGCGGGCATGATGGGCGGCGCGGTGCTGATCGTCGCCACGCCGA
TTGTCGGCGCCCTGGCCGACCGCAGCGGGCATCGCCCCTGGCTGCTGGGCTCGGCCATTGCCATCTTCCTCAGCGCCTAC
CCGATGTTTCTGTTCATCAACCAGATGCCAGGGCTGTTTTCACTGTTGATCTTCGAGTTGGTGCTCGGCCTGCTGATTTC
CGCTTACATCGGCTCGATCCTCGCAGCCTTCGGTGAATTGCTGCCCACCCACGTGCTGTCCACCGGGCTTTCGGTGGCCT
ACAACTTCGCCGTCACCCTCTTCGGCGGCTTTGCCACCTTCACCATCACCTGGCTGATTGCCTCGACCGGCAGCAACCTG
GCGCCAGCCTTTTACATCATGTTCGCCGCCCTCGTCAGCGGTATTGGCGCACTGCTTTATCGCGACCGTGCGCCTGCGGC
CCATCACCCCCTGTCTGGAGCTTACCCATGA

Upstream 100 bases:

>100_bases
AAAGACCTGACTCAGCCGCACTCACAACCAACCTGCCCCTCAATCAATGCAGCCGATGCAGGGGGCTTCCCTGCGTGTAT
CGCATGCAGGAGCTTATCCG

Downstream 100 bases:

>100_bases
GTAACACTGAACCCACACGCCTGATCCTGCGCAACGGCCGCCTGCTGGACGTGCAACAAGGGCAATTGATTTCGGGCCAG
GAGGTCGTGATCGAGGGTGA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTLQSSASRPHEQKARRAVVATVIGNGLEWFDFTVYSFFSVIIAKVFFPTGSELTSYLLALATFGVGFFMRPVGGIVLG
IYSDKRGRKAALSLTILLMALGTLIIGLTPGFAQIGYLAPLLIVLARLLQGFSAGGEMGSATAFLTEHAPEGRKAFYSSW
IQASIGVAVLLGSALGAVLSSYLTQAQLESWGWRVPFLIGTLIGPVGFYIRSRVDETPAYKAAKPVESPLREVIKTYPRQ
TLISFSLVVLWTVCTYAILFYIPSYAQRVLGLPSWMGFSAGMMGGAVLIVATPIVGALADRSGHRPWLLGSAIAIFLSAY
PMFLFINQMPGLFSLLIFELVLGLLISAYIGSILAAFGELLPTHVLSTGLSVAYNFAVTLFGGFATFTITWLIASTGSNL
APAFYIMFAALVSGIGALLYRDRAPAAHHPLSGAYP

Sequences:

>Translated_436_residues
MSTLQSSASRPHEQKARRAVVATVIGNGLEWFDFTVYSFFSVIIAKVFFPTGSELTSYLLALATFGVGFFMRPVGGIVLG
IYSDKRGRKAALSLTILLMALGTLIIGLTPGFAQIGYLAPLLIVLARLLQGFSAGGEMGSATAFLTEHAPEGRKAFYSSW
IQASIGVAVLLGSALGAVLSSYLTQAQLESWGWRVPFLIGTLIGPVGFYIRSRVDETPAYKAAKPVESPLREVIKTYPRQ
TLISFSLVVLWTVCTYAILFYIPSYAQRVLGLPSWMGFSAGMMGGAVLIVATPIVGALADRSGHRPWLLGSAIAIFLSAY
PMFLFINQMPGLFSLLIFELVLGLLISAYIGSILAAFGELLPTHVLSTGLSVAYNFAVTLFGGFATFTITWLIASTGSNL
APAFYIMFAALVSGIGALLYRDRAPAAHHPLSGAYP
>Mature_435_residues
STLQSSASRPHEQKARRAVVATVIGNGLEWFDFTVYSFFSVIIAKVFFPTGSELTSYLLALATFGVGFFMRPVGGIVLGI
YSDKRGRKAALSLTILLMALGTLIIGLTPGFAQIGYLAPLLIVLARLLQGFSAGGEMGSATAFLTEHAPEGRKAFYSSWI
QASIGVAVLLGSALGAVLSSYLTQAQLESWGWRVPFLIGTLIGPVGFYIRSRVDETPAYKAAKPVESPLREVIKTYPRQT
LISFSLVVLWTVCTYAILFYIPSYAQRVLGLPSWMGFSAGMMGGAVLIVATPIVGALADRSGHRPWLLGSAIAIFLSAYP
MFLFINQMPGLFSLLIFELVLGLLISAYIGSILAAFGELLPTHVLSTGLSVAYNFAVTLFGGFATFTITWLIASTGSNLA
PAFYIMFAALVSGIGALLYRDRAPAAHHPLSGAYP

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=444, Percent_Identity=33.7837837837838, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1788292, Length=433, Percent_Identity=31.8706697459584, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788942, Length=432, Percent_Identity=31.9444444444444, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=32.1678321678322, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87082231, Length=240, Percent_Identity=27.0833333333333, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46674; Mature: 46542

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLQSSASRPHEQKARRAVVATVIGNGLEWFDFTVYSFFSVIIAKVFFPTGSELTSYLL
CCCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALATFGVGFFMRPVGGIVLGIYSDKRGRKAALSLTILLMALGTLIIGLTPGFAQIGYLAP
HHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLIVLARLLQGFSAGGEMGSATAFLTEHAPEGRKAFYSSWIQASIGVAVLLGSALGAVLS
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLTQAQLESWGWRVPFLIGTLIGPVGFYIRSRVDETPAYKAAKPVESPLREVIKTYPRQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCHH
TLISFSLVVLWTVCTYAILFYIPSYAQRVLGLPSWMGFSAGMMGGAVLIVATPIVGALAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCHHHHHHHHHHHHHC
RSGHRPWLLGSAIAIFLSAYPMFLFINQMPGLFSLLIFELVLGLLISAYIGSILAAFGEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPTHVLSTGLSVAYNFAVTLFGGFATFTITWLIASTGSNLAPAFYIMFAALVSGIGALLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RDRAPAAHHPLSGAYP
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STLQSSASRPHEQKARRAVVATVIGNGLEWFDFTVYSFFSVIIAKVFFPTGSELTSYLL
CCCCHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALATFGVGFFMRPVGGIVLGIYSDKRGRKAALSLTILLMALGTLIIGLTPGFAQIGYLAP
HHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLIVLARLLQGFSAGGEMGSATAFLTEHAPEGRKAFYSSWIQASIGVAVLLGSALGAVLS
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLTQAQLESWGWRVPFLIGTLIGPVGFYIRSRVDETPAYKAAKPVESPLREVIKTYPRQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCHH
TLISFSLVVLWTVCTYAILFYIPSYAQRVLGLPSWMGFSAGMMGGAVLIVATPIVGALAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCHHHHHHHHHHHHHC
RSGHRPWLLGSAIAIFLSAYPMFLFINQMPGLFSLLIFELVLGLLISAYIGSILAAFGEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPTHVLSTGLSVAYNFAVTLFGGFATFTITWLIASTGSNLAPAFYIMFAALVSGIGALLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RDRAPAAHHPLSGAYP
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]