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The map label for this gene is gcvA [H]

Identifier: 66045768

GI number: 66045768

Start: 2944037

End: 2944942

Strand: Reverse

Name: gcvA [H]

Synonym: Psyr_2532

Alternate gene names: 66045768

Gene position: 2944942-2944037 (Counterclockwise)

Preceding gene: 66045770

Following gene: 66045767

Centisome position: 48.33

GC content: 63.58

Gene sequence:

>906_bases
ATGAACACCCGAAGACTGACGCCCTCCATGTCGCTGCTGCTCGCCTTCGAGGCGGCCGCCCGGCATGGCAGCTTCACCAA
GGCCGCAGACGAACTGGCCCTGACCCAGAGCGCCGTCAGCCGGCAGGTGCAGGCGCTGGAGGCACAACTGGAAGTCGAGC
TGTTCAAGCGCGACGGCAGACGCATCGAGCTGACTACCGCAGGCGCGCTGTATCAGCATGAGCTGGCTGCCGCGCTGGGC
CGCATCCGCAGTGCGACCCTGCAAACCATCGCGCACAAGGCCGAAGGCGGCACGCTGCATCTGGCCGTGCTGCCGACCTT
CGGCTCGAAGTGGCTGCTGCCACGCATGAATGACTTTTACACCCGGCATCCGGGCTATGTGGTGCACATCCACTCACGCA
TCGTGCATGCCGACCTGACGCCCGCCGCCAGCGAAATGAACGCGATAATCTGTGCCGGCAACGGCAACTGGCCCGGTTAC
ATCGCGCATCCGCTGGTCAGTGAAAAACTGGTGGTGATTGCCAGCCCGGCTGCCCTTGCCGGCTACAAGACGATGACCCC
GGCGCAGGTTGCCCAGCATGCGTTGCTAAGCGTGGTGTCGCGGCCCAATGCCTGGTCGGACTGGTTCGACAGTAATCGGC
TGGACCATCACATCATGCGCCCCGGTCCGAGCTTCGAACTGACCTCGCACCTGATCCAGGCCGTCGCGGCCGGCATTGGT
GTCGCGCTGGTCCCGCGCATTCTGGTGCAGGACGAAATCCACAGTGGCGAACTGGTCACCCTGTTCGAGCCCCTGGACAG
CGGACGCAATTATTACCTGGCCTACGCCACGCGTTTTCAGAACCTGCCATCGCTGTGCGTGTTCAGGGACTGGCTGCTGT
CGACGCCGTTTCCCGACACTTTGTAA

Upstream 100 bases:

>100_bases
ACGCTCGAATGCATGGCGTTATCAACGGTTAGCGTGATTCTCCTGCCTGTCATGCTTCAGTGCAAACGTAAAACCTGCCG
TTTTTCCATGAGTTTTATGA

Downstream 100 bases:

>100_bases
AACCTGCCCGCAACAGGAGCCACTGTGCGTATCGAGCCTCTTCCGCCGCTGCAGAACCTGATCGCTTTCGAAGCTGCCGT
CCGGCATGGCAGCTTCACGC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNTRRLTPSMSLLLAFEAAARHGSFTKAADELALTQSAVSRQVQALEAQLEVELFKRDGRRIELTTAGALYQHELAAALG
RIRSATLQTIAHKAEGGTLHLAVLPTFGSKWLLPRMNDFYTRHPGYVVHIHSRIVHADLTPAASEMNAIICAGNGNWPGY
IAHPLVSEKLVVIASPAALAGYKTMTPAQVAQHALLSVVSRPNAWSDWFDSNRLDHHIMRPGPSFELTSHLIQAVAAGIG
VALVPRILVQDEIHSGELVTLFEPLDSGRNYYLAYATRFQNLPSLCVFRDWLLSTPFPDTL

Sequences:

>Translated_301_residues
MNTRRLTPSMSLLLAFEAAARHGSFTKAADELALTQSAVSRQVQALEAQLEVELFKRDGRRIELTTAGALYQHELAAALG
RIRSATLQTIAHKAEGGTLHLAVLPTFGSKWLLPRMNDFYTRHPGYVVHIHSRIVHADLTPAASEMNAIICAGNGNWPGY
IAHPLVSEKLVVIASPAALAGYKTMTPAQVAQHALLSVVSRPNAWSDWFDSNRLDHHIMRPGPSFELTSHLIQAVAAGIG
VALVPRILVQDEIHSGELVTLFEPLDSGRNYYLAYATRFQNLPSLCVFRDWLLSTPFPDTL
>Mature_301_residues
MNTRRLTPSMSLLLAFEAAARHGSFTKAADELALTQSAVSRQVQALEAQLEVELFKRDGRRIELTTAGALYQHELAAALG
RIRSATLQTIAHKAEGGTLHLAVLPTFGSKWLLPRMNDFYTRHPGYVVHIHSRIVHADLTPAASEMNAIICAGNGNWPGY
IAHPLVSEKLVVIASPAALAGYKTMTPAQVAQHALLSVVSRPNAWSDWFDSNRLDHHIMRPGPSFELTSHLIQAVAAGIG
VALVPRILVQDEIHSGELVTLFEPLDSGRNYYLAYATRFQNLPSLCVFRDWLLSTPFPDTL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=35.7142857142857, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=29.7945205479452, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=28.7162162162162, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=28.3737024221453, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI157672245, Length=227, Percent_Identity=31.2775330396476, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789639, Length=273, Percent_Identity=27.1062271062271, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786401, Length=253, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=258, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786508, Length=59, Percent_Identity=54.2372881355932, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33057; Mature: 33057

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRRLTPSMSLLLAFEAAARHGSFTKAADELALTQSAVSRQVQALEAQLEVELFKRDGR
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIELTTAGALYQHELAAALGRIRSATLQTIAHKAEGGTLHLAVLPTFGSKWLLPRMNDFY
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHH
TRHPGYVVHIHSRIVHADLTPAASEMNAIICAGNGNWPGYIAHPLVSEKLVVIASPAALA
HCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEECCHHHC
GYKTMTPAQVAQHALLSVVSRPNAWSDWFDSNRLDHHIMRPGPSFELTSHLIQAVAAGIG
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCC
VALVPRILVQDEIHSGELVTLFEPLDSGRNYYLAYATRFQNLPSLCVFRDWLLSTPFPDT
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCC
L
C
>Mature Secondary Structure
MNTRRLTPSMSLLLAFEAAARHGSFTKAADELALTQSAVSRQVQALEAQLEVELFKRDGR
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIELTTAGALYQHELAAALGRIRSATLQTIAHKAEGGTLHLAVLPTFGSKWLLPRMNDFY
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHH
TRHPGYVVHIHSRIVHADLTPAASEMNAIICAGNGNWPGYIAHPLVSEKLVVIASPAALA
HCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEECCHHHC
GYKTMTPAQVAQHALLSVVSRPNAWSDWFDSNRLDHHIMRPGPSFELTSHLIQAVAAGIG
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCC
VALVPRILVQDEIHSGELVTLFEPLDSGRNYYLAYATRFQNLPSLCVFRDWLLSTPFPDT
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]