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The map label for this gene is mvaB [H]

Identifier: 66045707

GI number: 66045707

Start: 2865211

End: 2866110

Strand: Reverse

Name: mvaB [H]

Synonym: Psyr_2471

Alternate gene names: 66045707

Gene position: 2866110-2865211 (Counterclockwise)

Preceding gene: 66045708

Following gene: 66045706

Centisome position: 47.03

GC content: 63.44

Gene sequence:

>900_bases
ATGTCCCTCCCGCAAAAGGTGAATCTCGTTGAAGTCGGCCCGCGTGACGGTCTGCAGAATGAATCGCAACCCATCAGTGT
CGACGACAAGGTGCGCCTGGTGGATGACCTGACGGCAGCCGGGCTGGGCCATATCGAGGTCGGCAGCTTTGTCTCGGCCA
AATGGGTCCCGCAGATGGCGGGCTCGGCTCAGGTGTTCGAGCGGATCCAGCGCCGTGAAGGCGTTATCTATTCGGCTCTG
GCGCCGAATCTACGCGGCTTCGAAGACGCATTGGCTGCCGGGGTCAGAGAAGTCGCGGTGTTCGCGGCGGCGACCGAAGG
GTTCTCGCAGCGCAACCTCAACTGCTCGATCAGCGAAAGCCTGGAGCGCTTCGCGCCGGTCATGGAAGCGGCACGGATGC
ATGGCGTGCGGGTTCGAGGGTATGTGTCGTGCGTATTGGGCTGCCCTTATGAAGGCAGCGTTTCGCCCGAACAGGTCGCG
ACGGTCGCCAACCAACTGCACGCGATGGGCTGCTATGAAATTTCCCTGGGCGACACCATCGGAACCGGCACGCCGGGCGC
CACACGGGCCTTGTTCAACGCCGTGGCTGCGCAGATACCGCGCGGCAAGCTGGCCGGCCACTTCCACGACACCTATGGCC
AGGCGCTGGTAAACATCTACGCAAGCCTGGAGGAAGGCATCCAGGTCTTCGACAGCTCCGTGGCTGGCCTCGGCGGCTGC
CCCTACGCCAAGGGTGCCAGCGGCAATGTTGCCTCCGAGGACGTGCTGTACATGTTGCAGGGGCTGGGTATCGAAACAGG
CGTCGATCTGGACCAGGTCATTGCGGCGGGCCAGCGCATCTGCGACGTACTGCAACGTAGCAATGGCTCCCGCGTGGCCA
GAGCCAGACTGTCTGCGTAG

Upstream 100 bases:

>100_bases
ACTGGAACTGGACACCGCCGAAGAGCGCTGCCGAGCCGCACTCGATAAAACCGCTGCCGCCCCACTCTGACCCCTTCGCA
CTGACTACAAAGGCGCCGCC

Downstream 100 bases:

>100_bases
CACTGTCACCGGTCGGTAACAGGGTGCGACAACTTGCCACAGAGTGTTACTGTGCCCCGCGTCGAGCAGAGACAAACGGG
TAACACGGAAACACTCGGCC

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSLPQKVNLVEVGPRDGLQNESQPISVDDKVRLVDDLTAAGLGHIEVGSFVSAKWVPQMAGSAQVFERIQRREGVIYSAL
APNLRGFEDALAAGVREVAVFAAATEGFSQRNLNCSISESLERFAPVMEAARMHGVRVRGYVSCVLGCPYEGSVSPEQVA
TVANQLHAMGCYEISLGDTIGTGTPGATRALFNAVAAQIPRGKLAGHFHDTYGQALVNIYASLEEGIQVFDSSVAGLGGC
PYAKGASGNVASEDVLYMLQGLGIETGVDLDQVIAAGQRICDVLQRSNGSRVARARLSA

Sequences:

>Translated_299_residues
MSLPQKVNLVEVGPRDGLQNESQPISVDDKVRLVDDLTAAGLGHIEVGSFVSAKWVPQMAGSAQVFERIQRREGVIYSAL
APNLRGFEDALAAGVREVAVFAAATEGFSQRNLNCSISESLERFAPVMEAARMHGVRVRGYVSCVLGCPYEGSVSPEQVA
TVANQLHAMGCYEISLGDTIGTGTPGATRALFNAVAAQIPRGKLAGHFHDTYGQALVNIYASLEEGIQVFDSSVAGLGGC
PYAKGASGNVASEDVLYMLQGLGIETGVDLDQVIAAGQRICDVLQRSNGSRVARARLSA
>Mature_298_residues
SLPQKVNLVEVGPRDGLQNESQPISVDDKVRLVDDLTAAGLGHIEVGSFVSAKWVPQMAGSAQVFERIQRREGVIYSALA
PNLRGFEDALAAGVREVAVFAAATEGFSQRNLNCSISESLERFAPVMEAARMHGVRVRGYVSCVLGCPYEGSVSPEQVAT
VANQLHAMGCYEISLGDTIGTGTPGATRALFNAVAAQIPRGKLAGHFHDTYGQALVNIYASLEEGIQVFDSSVAGLGGCP
YAKGASGNVASEDVLYMLQGLGIETGVDLDQVIAAGQRICDVLQRSNGSRVARARLSA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=294, Percent_Identity=56.1224489795918, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI109150427, Length=297, Percent_Identity=55.2188552188552, Blast_Score=354, Evalue=4e-98,
Organism=Homo sapiens, GI109150422, Length=297, Percent_Identity=55.2188552188552, Blast_Score=354, Evalue=5e-98,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=58.0882352941177, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17510563, Length=295, Percent_Identity=49.1525423728814, Blast_Score=298, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24582381, Length=294, Percent_Identity=57.4829931972789, Blast_Score=357, Evalue=5e-99,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 31512; Mature: 31381

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPQKVNLVEVGPRDGLQNESQPISVDDKVRLVDDLTAAGLGHIEVGSFVSAKWVPQMA
CCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHC
GSAQVFERIQRREGVIYSALAPNLRGFEDALAAGVREVAVFAAATEGFSQRNLNCSISES
CCHHHHHHHHHHCCEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHH
LERFAPVMEAARMHGVRVRGYVSCVLGCPYEGSVSPEQVATVANQLHAMGCYEISLGDTI
HHHHHHHHHHHHHCCEEHHHHEEHEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCC
GTGTPGATRALFNAVAAQIPRGKLAGHFHDTYGQALVNIYASLEEGIQVFDSSVAGLGGC
CCCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYAKGASGNVASEDVLYMLQGLGIETGVDLDQVIAAGQRICDVLQRSNGSRVARARLSA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
SLPQKVNLVEVGPRDGLQNESQPISVDDKVRLVDDLTAAGLGHIEVGSFVSAKWVPQMA
CCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHC
GSAQVFERIQRREGVIYSALAPNLRGFEDALAAGVREVAVFAAATEGFSQRNLNCSISES
CCHHHHHHHHHHCCEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHH
LERFAPVMEAARMHGVRVRGYVSCVLGCPYEGSVSPEQVATVANQLHAMGCYEISLGDTI
HHHHHHHHHHHHHCCEEHHHHEEHEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCC
GTGTPGATRALFNAVAAQIPRGKLAGHFHDTYGQALVNIYASLEEGIQVFDSSVAGLGGC
CCCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYAKGASGNVASEDVLYMLQGLGIETGVDLDQVIAAGQRICDVLQRSNGSRVARARLSA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]