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The map label for this gene is rnhA [H]

Identifier: 66045008

GI number: 66045008

Start: 2004697

End: 2005149

Strand: Direct

Name: rnhA [H]

Synonym: Psyr_1763

Alternate gene names: 66045008

Gene position: 2004697-2005149 (Clockwise)

Preceding gene: 66045007

Following gene: 66045009

Centisome position: 32.9

GC content: 61.15

Gene sequence:

>453_bases
ATGAGCGATAGCGTTGAACTCTTCACCGACGGTGCCTGCAAGGGTAATCCTGGCCCGGGCGGGTGGGGAGCCCTGCTGGT
GTGCAAGGGCGTCGAAAAAGAGTTGTGGGGTGGCGAAGCCAATACCACCAACAACCGGATGGAACTGACCGGTGCGATTC
GCGGGCTTGAAGAACTCAAGCGCCCCTGCGAAGTGACGCTGGTGACCGACTCTCAGTACGTGATGAAAGGCATTACCGAG
TGGATGGTCAACTGGAAGAAGCGCGGCTGGAAAACCGCTGCCAAGGAGCCGGTCAAAAATGCCGACCTGTGGCAGCTTCT
GGATGAACAGGTCAGTCGCCACACGGTGAAATGGCAGTGGGTGCGTGGGCACATCGGCCACCCTGGCAACGAGCGTGCCG
ACCAGCTCGCCAACCGTGGCGTCGACGAAGTGCGCGGCATCCGGCACGGCTAG

Upstream 100 bases:

>100_bases
CTGATTCCCATGCCGATGGCCAAGGTCAGCCGCAATACTCAGGATCAGGCTTGAGCAATGCCATGCCGCAAATGAAGCCC
ATCATTCTGGAAAGGTCGTA

Downstream 100 bases:

>100_bases
ACGCGCACGGGCTTGCAACGGCCGCTGCGTGCTAGAATCGCGGCCTGTACATGAGGCAACAGTCAATGGCATTGCAAAAC
CTGGATAACAGATCGATCGT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HI [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MSDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELTGAIRGLEELKRPCEVTLVTDSQYVMKGITE
WMVNWKKRGWKTAAKEPVKNADLWQLLDEQVSRHTVKWQWVRGHIGHPGNERADQLANRGVDEVRGIRHG

Sequences:

>Translated_150_residues
MSDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELTGAIRGLEELKRPCEVTLVTDSQYVMKGITE
WMVNWKKRGWKTAAKEPVKNADLWQLLDEQVSRHTVKWQWVRGHIGHPGNERADQLANRGVDEVRGIRHG
>Mature_149_residues
SDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELTGAIRGLEELKRPCEVTLVTDSQYVMKGITEW
MVNWKKRGWKTAAKEPVKNADLWQLLDEQVSRHTVKWQWVRGHIGHPGNERADQLANRGVDEVRGIRHG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=148, Percent_Identity=34.4594594594595, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1786408, Length=137, Percent_Identity=63.5036496350365, Blast_Score=185, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=35, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=36.986301369863, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=36.986301369863, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16777; Mature: 16646

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELTGAIRGLEELK
CCCCCCEEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHC
RPCEVTLVTDSQYVMKGITEWMVNWKKRGWKTAAKEPVKNADLWQLLDEQVSRHTVKWQW
CCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCEEEEE
VRGHIGHPGNERADQLANRGVDEVRGIRHG
EECCCCCCCCHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure 
SDSVELFTDGACKGNPGPGGWGALLVCKGVEKELWGGEANTTNNRMELTGAIRGLEELK
CCCCCEEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHC
RPCEVTLVTDSQYVMKGITEWMVNWKKRGWKTAAKEPVKNADLWQLLDEQVSRHTVKWQW
CCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCEEEEE
VRGHIGHPGNERADQLANRGVDEVRGIRHG
EECCCCCCCCHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]