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The map label for this gene is eptA [H]

Identifier: 66044807

GI number: 66044807

Start: 1758149

End: 1759825

Strand: Direct

Name: eptA [H]

Synonym: Psyr_1562

Alternate gene names: 66044807

Gene position: 1758149-1759825 (Clockwise)

Preceding gene: 229170802

Following gene: 66044808

Centisome position: 28.85

GC content: 54.08

Gene sequence:

>1677_bases
ATGCCCGATGCATATTCATTCGATAAGCAGACCACGATGCCGACACCCAGAACTGTTCGTCCAGAAGTTGTAACCCTACT
TGCCAGTGCGTTTCTGTTACTGGGTTTCAATATCAATCTCTGGCAGCATCTTTTCTCGATTACCAGCGCCGATAGTAAAG
GGTTGGCCATGCGCTTTGCGTTTGGCTTGATGATTTTCTGCGTGTTCAATATCGTGCTGACGCTGTTGGCATTCCGTCGT
GTGTTTAAACCGGTGTTGATCAGTCTGTTTATGATCAGCGCGGGGGTGGTTTATTTCATGACCGAATACGGGGTCATGAT
CGACGCTGGCATGTTTCGAAACTTCGCCGAAACCAACGTTACGGAAGTGCAGGGGCTGCTGTCTTTAAAACTGGCCTTGT
ATATCCTTCTGCTCGGCGTAGTGCCCTCGCTGATACTCTGGAAACTGCCGATCAGCTACCGTCGCTGGCACCTTGAACTG
TTCAGCAAACTGCTGGTGACGGTTGCGTGCTGCGTGGCAATCGGCGGCGTTGCACTGGCCAATTACCAGGGGCTGTCGTC
ATTGTTTCGCAATCACCATGAGTTGCGCCTGATGGTGGTGCCAAGCAATTACATTGGTGCGTCGCTGGGCTACCTGAGTG
AGCAGGTGCTCTCTGCAAGGCGCCCGTTCGTCAAGATCGGCGAGGACGCCAAACGTTCTGCGGATTGGGCCACGCATGCT
CGAAAATCGTTGACCGTGCTGGTGGTCGGCGAAAGTGCCCGGGCCGAGAACTTCGGGATCCTTGGCTATGGTCGTAACAC
CACGCCCAACCTCAACAAGGAAAGCGGGCTGGTGGCGTTTACCGATGTGTATTCCTGCGGCACCGAAACCGCCGTGTCGG
TGCCTTGCATGTTCTCCAACATGGGGCGCAAGAATTACAACGCTACCCAGGCGCGCAATCAGGAAGGCTTGCTGGATGTA
CTGAAACGCGCGGGCATAGACGTGATATGGCGTGACAATCAGGCCGGCTGCAAAGGCACCTGTGATCGAGTGACCTTCCA
GGACGTGAGCAATCTCAAGGACCCGCTGTTGTGCAACAACCATGAATGCCGGGACGAGATACTCTTGCAAAACCTGCAGA
CCTTTATCGACAGCCTCGACAAGGACACGGTGCTTGTTCTGCACCAGATGGGCAGCCACGGGCCTGAATACTACAAACGT
TACCCAAAAGAGTTTGAAAAGTTTACCCCGGTGTGTGAAAGCAATGCGCTGAACAATTGCAGCCGGGACAGCATCGTCAA
TGCGTACGACAATACGTTGCTTTACACCGACCATGTACTGTCCACGCTGATTGATCTTTTACGCGCCAATCAAAACAAGG
TCGATACCGCCATGGTCTACCTGTCGGACCATGGCGAGTCGCTGGGCGAGTACAACCTGTTCCTGCACGGCACGCCTTAT
GTGCTGGCGCCTGATCAGCAAAAGCATGTGCCGATGATGGTCTGGTTGTCCGATACCTATCAGAAGTCCTTTGCCGTCAC
GCCGGAGTGCCTGGCCAAACAGCGTAACGCACCGTTGAGTCAGGACAATCTTTTCCATTCCATGCTCGGCCTGCTGAAAG
TCGACACCAAGGTCTATGACCCCAGTCTCGATATGTTTGCCGGATGCCGCAGCGAGTTGGCGGCCACCGACAAGTAA

Upstream 100 bases:

>100_bases
GAAATAAGCTTCATTCAACCCAATCCTCTCACCATTTTCACATAAAATTGATGATGCGCTGCCTACAGTGCGCGCCATCG
GGGAGTTCACTGGCTCCTGA

Downstream 100 bases:

>100_bases
CCACGCGCACGGTGTTCCGGGTATTGATCGTCCTCTCACGTTATGGGGACGATCATCGGCAGCGCGTTTGCCCGGCAGTC
GCTGTTCCCGTCCGTCATTT

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MPDAYSFDKQTTMPTPRTVRPEVVTLLASAFLLLGFNINLWQHLFSITSADSKGLAMRFAFGLMIFCVFNIVLTLLAFRR
VFKPVLISLFMISAGVVYFMTEYGVMIDAGMFRNFAETNVTEVQGLLSLKLALYILLLGVVPSLILWKLPISYRRWHLEL
FSKLLVTVACCVAIGGVALANYQGLSSLFRNHHELRLMVVPSNYIGASLGYLSEQVLSARRPFVKIGEDAKRSADWATHA
RKSLTVLVVGESARAENFGILGYGRNTTPNLNKESGLVAFTDVYSCGTETAVSVPCMFSNMGRKNYNATQARNQEGLLDV
LKRAGIDVIWRDNQAGCKGTCDRVTFQDVSNLKDPLLCNNHECRDEILLQNLQTFIDSLDKDTVLVLHQMGSHGPEYYKR
YPKEFEKFTPVCESNALNNCSRDSIVNAYDNTLLYTDHVLSTLIDLLRANQNKVDTAMVYLSDHGESLGEYNLFLHGTPY
VLAPDQQKHVPMMVWLSDTYQKSFAVTPECLAKQRNAPLSQDNLFHSMLGLLKVDTKVYDPSLDMFAGCRSELAATDK

Sequences:

>Translated_558_residues
MPDAYSFDKQTTMPTPRTVRPEVVTLLASAFLLLGFNINLWQHLFSITSADSKGLAMRFAFGLMIFCVFNIVLTLLAFRR
VFKPVLISLFMISAGVVYFMTEYGVMIDAGMFRNFAETNVTEVQGLLSLKLALYILLLGVVPSLILWKLPISYRRWHLEL
FSKLLVTVACCVAIGGVALANYQGLSSLFRNHHELRLMVVPSNYIGASLGYLSEQVLSARRPFVKIGEDAKRSADWATHA
RKSLTVLVVGESARAENFGILGYGRNTTPNLNKESGLVAFTDVYSCGTETAVSVPCMFSNMGRKNYNATQARNQEGLLDV
LKRAGIDVIWRDNQAGCKGTCDRVTFQDVSNLKDPLLCNNHECRDEILLQNLQTFIDSLDKDTVLVLHQMGSHGPEYYKR
YPKEFEKFTPVCESNALNNCSRDSIVNAYDNTLLYTDHVLSTLIDLLRANQNKVDTAMVYLSDHGESLGEYNLFLHGTPY
VLAPDQQKHVPMMVWLSDTYQKSFAVTPECLAKQRNAPLSQDNLFHSMLGLLKVDTKVYDPSLDMFAGCRSELAATDK
>Mature_557_residues
PDAYSFDKQTTMPTPRTVRPEVVTLLASAFLLLGFNINLWQHLFSITSADSKGLAMRFAFGLMIFCVFNIVLTLLAFRRV
FKPVLISLFMISAGVVYFMTEYGVMIDAGMFRNFAETNVTEVQGLLSLKLALYILLLGVVPSLILWKLPISYRRWHLELF
SKLLVTVACCVAIGGVALANYQGLSSLFRNHHELRLMVVPSNYIGASLGYLSEQVLSARRPFVKIGEDAKRSADWATHAR
KSLTVLVVGESARAENFGILGYGRNTTPNLNKESGLVAFTDVYSCGTETAVSVPCMFSNMGRKNYNATQARNQEGLLDVL
KRAGIDVIWRDNQAGCKGTCDRVTFQDVSNLKDPLLCNNHECRDEILLQNLQTFIDSLDKDTVLVLHQMGSHGPEYYKRY
PKEFEKFTPVCESNALNNCSRDSIVNAYDNTLLYTDHVLSTLIDLLRANQNKVDTAMVYLSDHGESLGEYNLFLHGTPYV
LAPDQQKHVPMMVWLSDTYQKSFAVTPECLAKQRNAPLSQDNLFHSMLGLLKVDTKVYDPSLDMFAGCRSELAATDK

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=524, Percent_Identity=44.0839694656489, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI87082286, Length=487, Percent_Identity=27.9260780287474, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI87082223, Length=256, Percent_Identity=27.34375, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790392, Length=311, Percent_Identity=26.6881028938907, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787035, Length=266, Percent_Identity=27.0676691729323, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62476; Mature: 62345

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAYSFDKQTTMPTPRTVRPEVVTLLASAFLLLGFNINLWQHLFSITSADSKGLAMRFA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
FGLMIFCVFNIVLTLLAFRRVFKPVLISLFMISAGVVYFMTEYGVMIDAGMFRNFAETNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCH
TEVQGLLSLKLALYILLLGVVPSLILWKLPISYRRWHLELFSKLLVTVACCVAIGGVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYQGLSSLFRNHHELRLMVVPSNYIGASLGYLSEQVLSARRPFVKIGEDAKRSADWATHA
HHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
RKSLTVLVVGESARAENFGILGYGRNTTPNLNKESGLVAFTDVYSCGTETAVSVPCMFSN
HCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHCCCCCCEECCHHHHH
MGRKNYNATQARNQEGLLDVLKRAGIDVIWRDNQAGCKGTCDRVTFQDVSNLKDPLLCNN
CCCCCCCHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
HECRDEILLQNLQTFIDSLDKDTVLVLHQMGSHGPEYYKRYPKEFEKFTPVCESNALNNC
CCHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCHHHHHHCHHHHHHHCCCHHCCCCCCC
SRDSIVNAYDNTLLYTDHVLSTLIDLLRANQNKVDTAMVYLSDHGESLGEYNLFLHGTPY
CHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEECCCE
VLAPDQQKHVPMMVWLSDTYQKSFAVTPECLAKQRNAPLSQDNLFHSMLGLLKVDTKVYD
EECCCCCCCCCEEEEECCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
PSLDMFAGCRSELAATDK
CCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PDAYSFDKQTTMPTPRTVRPEVVTLLASAFLLLGFNINLWQHLFSITSADSKGLAMRFA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
FGLMIFCVFNIVLTLLAFRRVFKPVLISLFMISAGVVYFMTEYGVMIDAGMFRNFAETNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCH
TEVQGLLSLKLALYILLLGVVPSLILWKLPISYRRWHLELFSKLLVTVACCVAIGGVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYQGLSSLFRNHHELRLMVVPSNYIGASLGYLSEQVLSARRPFVKIGEDAKRSADWATHA
HHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
RKSLTVLVVGESARAENFGILGYGRNTTPNLNKESGLVAFTDVYSCGTETAVSVPCMFSN
HCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHCCCCCCEECCHHHHH
MGRKNYNATQARNQEGLLDVLKRAGIDVIWRDNQAGCKGTCDRVTFQDVSNLKDPLLCNN
CCCCCCCHHHHCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
HECRDEILLQNLQTFIDSLDKDTVLVLHQMGSHGPEYYKRYPKEFEKFTPVCESNALNNC
CCHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCHHHHHHCHHHHHHHCCCHHCCCCCCC
SRDSIVNAYDNTLLYTDHVLSTLIDLLRANQNKVDTAMVYLSDHGESLGEYNLFLHGTPY
CHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEEECCCE
VLAPDQQKHVPMMVWLSDTYQKSFAVTPECLAKQRNAPLSQDNLFHSMLGLLKVDTKVYD
EECCCCCCCCCEEEEECCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
PSLDMFAGCRSELAATDK
CCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]