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The map label for this gene is tapB [H]

Identifier: 66044345

GI number: 66044345

Start: 1236770

End: 1236982

Strand: Reverse

Name: tapB [H]

Synonym: Psyr_1094

Alternate gene names: 66044345

Gene position: 1236982-1236770 (Counterclockwise)

Preceding gene: 66044346

Following gene: 66044342

Centisome position: 20.3

GC content: 52.11

Gene sequence:

>213_bases
ATGGCTGAACGTCAGAGCGGTACCGTCAAGTGGTTCAACGACGAAAAAGGTTTTGGTTTTATCACTCCTGAGAGCGGGCC
GGATCTGTTCGTACACTTCCGCGCTATTCAGGGTAACGGCTTCAAGAGCCTGAAAGAAGGCCAGAAAGTTACCTTCGTTG
CAGTTCAGGGCCAGAAAGGCATGCAGGCTGACGAAGTTCAAGCCGAAGGCTGA

Upstream 100 bases:

>100_bases
AACCCGTTCTACGTTTCTTACTTCCTGCAACCAGCCCCAGTGCTCTTTCATGCGAAAGAGGCTGTAACCAATTCAAGTCA
AGCGTCAAGGAAATAAGAAA

Downstream 100 bases:

>100_bases
GAACGACGCACCACGCATTTACAAGAAAGCCTCTGGCAGCGATGCCAGAGGCTTTTTTGCATGCGGGATTAGCAAGCGCC
CATGAAAAAGCCGACCCGGG

Product: cold-shock protein, DNA-binding

Products: NA

Alternate protein names: E8.0 [H]

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MAERQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKGMQADEVQAEG

Sequences:

>Translated_70_residues
MAERQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKGMQADEVQAEG
>Mature_69_residues
AERQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKGMQADEVQAEG

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=69, Percent_Identity=56.5217391304348, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=63.4920634920635, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=56.7164179104478, Blast_Score=77, Evalue=1e-16,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=65.0793650793651, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787834, Length=69, Percent_Identity=56.5217391304348, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=53.7313432835821, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1787107, Length=63, Percent_Identity=52.3809523809524, Blast_Score=69, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7693; Mature: 7562

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKG
CCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
MQADEVQAEG
CCCCCEECCC
>Mature Secondary Structure 
AERQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKG
CCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
MQADEVQAEG
CCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9393697 [H]