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The map label for this gene is nemA [C]

Identifier: 66044264

GI number: 66044264

Start: 1145082

End: 1146185

Strand: Direct

Name: nemA [C]

Synonym: Psyr_1011

Alternate gene names: 66044264

Gene position: 1145082-1146185 (Clockwise)

Preceding gene: 66044263

Following gene: 66044269

Centisome position: 18.79

GC content: 62.68

Gene sequence:

>1104_bases
ATGTCGGCAGAAGCCTTGTTCACGCCTTTCCGTCTGGGCAGCCTGGAGCTGTCCTCGCGTGTGGTCATGGCCCCCATGAC
CCGCTCGTTCTCGCCAGGGCACGTGCCCAACTCGAAGGTCGTCGAGTACTACCGGCGGCGCGCTGCTGCAGGCGTCGGGC
TGATTATCACCGAAGGTACGACGGTCAATCACAAGGCCTCCAACGGTTATCCGAACGTCCCGCAGTTTTTCGGCGAAGCG
CCGCTGGCCGGCTGGCGCAAGGTGGTCGAGGCGGTGCATGCGGAGGGCGGCAAGATTGTTCCGCAACTCTGGCACGTCGG
CGCAGTTCGCCGTCCTGGCACCGAGCCGGACGGCAGCGTGCCTGCCTACGGGCCGATGGAGAAGGTCAAGGACGGTCAGG
TGCTGGTGCATGGCATGAGCAAGCAGGACATCGACGAGGTCGTAGCGGCCTTTGCCCAGGCTGCCGCCGATGCCAGGGCG
ATTGGCATGGACGGTGTCGAAATTCATGGGGCTCACGGTTACCTGGTCGATCAGTTCTTTTGGGAGGGCAGCAATCAGCG
TAGCGACGAGTACGGCGGCAGCCTCGCCAACCGCTCACGGTTTGCCCTCGAACTGATCAAGGCCGTGCGCGGCGCAGTAG
GCCCGGACTACCCGATCATTTTCCGTTTCTCACAATGGAAACAGCAGGATTACACCGCGCGCCTGGTGCAAACGCCCGAG
GCGCTGGGCGAGTTCCTGCAGCCTCTGGCTGATGCAGGTGTGGATATCTTTCATTGCTCTACCCGGCGTTTCTGGGAGCC
TGAGTTTGAAGGCTCCGACCTGAACCTGGCGGGCTGGACGCGCAAGCTGACCGGCAAACCGACCATTACTGTCGGCAGCG
TCGGACTGGACGGCGAGTTCCTGCAATTCATGGTCAACACCGACAAGGTCGCTCAACCGGCCAGCCTGGAAAATCTGCTG
AAGCGACTGGGCAACGACGAGTTCGATCTGGTGGCGGTGGGGCGCGCGCTGCTGGTCGATCCTGATTGGGCACTGAAAGT
GCGCGAGGGGCGCGAGCAGGATATCCTGCCCTTCAGTCGAGACGCACTGACGACACTGGTCTGA

Upstream 100 bases:

>100_bases
CTGAACTCAAATCAGCCAGATCAATAGTACTGGGCCGCCGCTGACGCCCGGCGATATTCTGCGCGCCTGATGGCAATCGA
CCATTTCACAGGAGTTGCGC

Downstream 100 bases:

>100_bases
TTTTCTTGCTGGCTACCGACCTGATCTCTTTGACGCTTGCAGCGTTCTGGCTCGGGCAGGCCTCGCGCAATTGACGCTCG
AACTGATCGATGACCGCTGT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSAEALFTPFRLGSLELSSRVVMAPMTRSFSPGHVPNSKVVEYYRRRAAAGVGLIITEGTTVNHKASNGYPNVPQFFGEA
PLAGWRKVVEAVHAEGGKIVPQLWHVGAVRRPGTEPDGSVPAYGPMEKVKDGQVLVHGMSKQDIDEVVAAFAQAAADARA
IGMDGVEIHGAHGYLVDQFFWEGSNQRSDEYGGSLANRSRFALELIKAVRGAVGPDYPIIFRFSQWKQQDYTARLVQTPE
ALGEFLQPLADAGVDIFHCSTRRFWEPEFEGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLENLL
KRLGNDEFDLVAVGRALLVDPDWALKVREGREQDILPFSRDALTTLV

Sequences:

>Translated_367_residues
MSAEALFTPFRLGSLELSSRVVMAPMTRSFSPGHVPNSKVVEYYRRRAAAGVGLIITEGTTVNHKASNGYPNVPQFFGEA
PLAGWRKVVEAVHAEGGKIVPQLWHVGAVRRPGTEPDGSVPAYGPMEKVKDGQVLVHGMSKQDIDEVVAAFAQAAADARA
IGMDGVEIHGAHGYLVDQFFWEGSNQRSDEYGGSLANRSRFALELIKAVRGAVGPDYPIIFRFSQWKQQDYTARLVQTPE
ALGEFLQPLADAGVDIFHCSTRRFWEPEFEGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLENLL
KRLGNDEFDLVAVGRALLVDPDWALKVREGREQDILPFSRDALTTLV
>Mature_366_residues
SAEALFTPFRLGSLELSSRVVMAPMTRSFSPGHVPNSKVVEYYRRRAAAGVGLIITEGTTVNHKASNGYPNVPQFFGEAP
LAGWRKVVEAVHAEGGKIVPQLWHVGAVRRPGTEPDGSVPAYGPMEKVKDGQVLVHGMSKQDIDEVVAAFAQAAADARAI
GMDGVEIHGAHGYLVDQFFWEGSNQRSDEYGGSLANRSRFALELIKAVRGAVGPDYPIIFRFSQWKQQDYTARLVQTPEA
LGEFLQPLADAGVDIFHCSTRRFWEPEFEGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLENLLK
RLGNDEFDLVAVGRALLVDPDWALKVREGREQDILPFSRDALTTLV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=377, Percent_Identity=31.5649867374005, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789463, Length=363, Percent_Identity=29.4765840220386, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17566914, Length=199, Percent_Identity=30.6532663316583, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565138, Length=209, Percent_Identity=30.1435406698565, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559804, Length=191, Percent_Identity=30.3664921465969, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=68, Percent_Identity=50, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=223, Percent_Identity=27.8026905829596, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17559802, Length=74, Percent_Identity=44.5945945945946, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540738, Length=212, Percent_Identity=27.3584905660377, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=224, Percent_Identity=39.7321428571429, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325086, Length=375, Percent_Identity=31.2, Blast_Score=145, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40195; Mature: 40064

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEALFTPFRLGSLELSSRVVMAPMTRSFSPGHVPNSKVVEYYRRRAAAGVGLIITEGT
CCCCCEECCCCCCCEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
TVNHKASNGYPNVPQFFGEAPLAGWRKVVEAVHAEGGKIVPQLWHVGAVRRPGTEPDGSV
EECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCC
PAYGPMEKVKDGQVLVHGMSKQDIDEVVAAFAQAAADARAIGMDGVEIHGAHGYLVDQFF
CCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
WEGSNQRSDEYGGSLANRSRFALELIKAVRGAVGPDYPIIFRFSQWKQQDYTARLVQTPE
HCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHHHHCCHH
ALGEFLQPLADAGVDIFHCSTRRFWEPEFEGSDLNLAGWTRKLTGKPTITVGSVGLDGEF
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEECCCEEECCCCCEEEEECCCCCHHH
LQFMVNTDKVAQPASLENLLKRLGNDEFDLVAVGRALLVDPDWALKVREGREQDILPFSR
HHHEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCEEEECCCCCCCCCCCCH
DALTTLV
HHHHHCC
>Mature Secondary Structure 
SAEALFTPFRLGSLELSSRVVMAPMTRSFSPGHVPNSKVVEYYRRRAAAGVGLIITEGT
CCCCEECCCCCCCEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
TVNHKASNGYPNVPQFFGEAPLAGWRKVVEAVHAEGGKIVPQLWHVGAVRRPGTEPDGSV
EECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCC
PAYGPMEKVKDGQVLVHGMSKQDIDEVVAAFAQAAADARAIGMDGVEIHGAHGYLVDQFF
CCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
WEGSNQRSDEYGGSLANRSRFALELIKAVRGAVGPDYPIIFRFSQWKQQDYTARLVQTPE
HCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHHHHCCHH
ALGEFLQPLADAGVDIFHCSTRRFWEPEFEGSDLNLAGWTRKLTGKPTITVGSVGLDGEF
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEECCCEEECCCCCEEEEECCCCCHHH
LQFMVNTDKVAQPASLENLLKRLGNDEFDLVAVGRALLVDPDWALKVREGREQDILPFSR
HHHEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCEEEECCCCCCCCCCCCH
DALTTLV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]