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The map label for this gene is rutR [H]

Identifier: 66044253

GI number: 66044253

Start: 1133764

End: 1134492

Strand: Direct

Name: rutR [H]

Synonym: Psyr_1000

Alternate gene names: 66044253

Gene position: 1133764-1134492 (Clockwise)

Preceding gene: 66044246

Following gene: 66044254

Centisome position: 18.61

GC content: 62.14

Gene sequence:

>729_bases
ATGTGCGTGAACAAGAAACCTCCCCTGGCTGACCCGGCGAACAAACGCGTGCGCAAGGCCAAGCCAGAGACCGTGGTCAA
GACGACCCGCGAACCCAGTGCGACGTCCCTGAAGCGGCGCATGCGCCTGATGGAAAGCAAGCGCACGCTGATTCTCGATG
CGGCGCTGGAGATATTTTCCCGCTATGGCGTGCATGGCAGCAGCCTGGATCAGGTCGCGAGCCTGGCGGATGTCTCCAAG
ACCAACCTGCTGTATTACTTCAGCAGCAAGGACGACCTGTACCTGAACGTGCTGCGCCAGCTGCTCGAGGTATGGCTGAG
CCCGCTGGTGCACTTCACCGCCGACAAGGAGCCGGTGCAGGCGATCAGCGCCTACATCAAGGCCAAGCTGGAGATGTCCC
GCGATCACCCGGCCGAATCCCGGCTGTTCTGCATGGAAGTGATGCAGGGCGCACCGCTGATTCAGGGCGAGTTGCAGCAC
CCGTTGCGTGACACGGTGCAGGCCAAGGTGGCGGTGATTCAGCACTGGATCGACAGCGGTCAGCTGGCCCCCATCAATCC
GCACCACCTGATTTTTACGCTGTGGGCGACCACCCAGCATTACGCCGACTTCCGTACTCAGGTCGAGGCCGTGACCGGCA
AGACCCTGGATGATCCGGCGTTTTTCGACGAGGTGCTGACGAGCCTGCGCAGCATGGTTCTGGACGGGATCCTGCCGCGC
AAGGCGTGA

Upstream 100 bases:

>100_bases
GGCGCACGCAGATGGGGCAGGGCGGACGGCGCAGGCAATCCGTATCGACAAAACAGCTGCGCCCGTTAAGATGCCCGCTC
ATTTCGGCATCACTGGATGG

Downstream 100 bases:

>100_bases
ATGGACCTCGCCGGACCTGACCCGCGCACCCCGCTCGCGCAGTGCGCGGGCGTCCTGCACAAACCAAGTGCTCCGATTCA
GACCATTTGATCTGCTTTCT

Product: regulatory protein, TetR

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MCVNKKPPLADPANKRVRKAKPETVVKTTREPSATSLKRRMRLMESKRTLILDAALEIFSRYGVHGSSLDQVASLADVSK
TNLLYYFSSKDDLYLNVLRQLLEVWLSPLVHFTADKEPVQAISAYIKAKLEMSRDHPAESRLFCMEVMQGAPLIQGELQH
PLRDTVQAKVAVIQHWIDSGQLAPINPHHLIFTLWATTQHYADFRTQVEAVTGKTLDDPAFFDEVLTSLRSMVLDGILPR
KA

Sequences:

>Translated_242_residues
MCVNKKPPLADPANKRVRKAKPETVVKTTREPSATSLKRRMRLMESKRTLILDAALEIFSRYGVHGSSLDQVASLADVSK
TNLLYYFSSKDDLYLNVLRQLLEVWLSPLVHFTADKEPVQAISAYIKAKLEMSRDHPAESRLFCMEVMQGAPLIQGELQH
PLRDTVQAKVAVIQHWIDSGQLAPINPHHLIFTLWATTQHYADFRTQVEAVTGKTLDDPAFFDEVLTSLRSMVLDGILPR
KA
>Mature_242_residues
MCVNKKPPLADPANKRVRKAKPETVVKTTREPSATSLKRRMRLMESKRTLILDAALEIFSRYGVHGSSLDQVASLADVSK
TNLLYYFSSKDDLYLNVLRQLLEVWLSPLVHFTADKEPVQAISAYIKAKLEMSRDHPAESRLFCMEVMQGAPLIQGELQH
PLRDTVQAKVAVIQHWIDSGQLAPINPHHLIFTLWATTQHYADFRTQVEAVTGKTLDDPAFFDEVLTSLRSMVLDGILPR
KA

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=202, Percent_Identity=53.960396039604, Blast_Score=239, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 27334; Mature: 27334

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVNKKPPLADPANKRVRKAKPETVVKTTREPSATSLKRRMRLMESKRTLILDAALEIFS
CCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGVHGSSLDQVASLADVSKTNLLYYFSSKDDLYLNVLRQLLEVWLSPLVHFTADKEPVQ
HHCCCCCCHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AISAYIKAKLEMSRDHPAESRLFCMEVMQGAPLIQGELQHPLRDTVQAKVAVIQHWIDSG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
QLAPINPHHLIFTLWATTQHYADFRTQVEAVTGKTLDDPAFFDEVLTSLRSMVLDGILPR
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KA
CC
>Mature Secondary Structure
MCVNKKPPLADPANKRVRKAKPETVVKTTREPSATSLKRRMRLMESKRTLILDAALEIFS
CCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGVHGSSLDQVASLADVSKTNLLYYFSSKDDLYLNVLRQLLEVWLSPLVHFTADKEPVQ
HHCCCCCCHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AISAYIKAKLEMSRDHPAESRLFCMEVMQGAPLIQGELQHPLRDTVQAKVAVIQHWIDSG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
QLAPINPHHLIFTLWATTQHYADFRTQVEAVTGKTLDDPAFFDEVLTSLRSMVLDGILPR
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]