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The map label for this gene is lolB [H]

Identifier: 66044199

GI number: 66044199

Start: 1081051

End: 1081668

Strand: Reverse

Name: lolB [H]

Synonym: Psyr_0946

Alternate gene names: 66044199

Gene position: 1081668-1081051 (Counterclockwise)

Preceding gene: 66044200

Following gene: 66044198

Centisome position: 17.75

GC content: 62.78

Gene sequence:

>618_bases
ATGTTTTTGCGCCACGTAATCGTATTCAGCCTCATCGCCCTGCTCACCGGCTGCGCCGGCCTCACCTCCCGCGAAGCCGT
TCAGGGCAAGGGTGACCCCGCGCAATGGCGCGAGCACAAACAGCAACTGAGCAGCCTCGATGGCTGGCAGATCAACGGCA
AGGTCGGTATCCGCGCGCCGAAAGACTCCGGCAGCGGCACCCTGTTCTGGCTGCAGCGCCAGGATTACTACGACATCCGC
CTGTCCGGCCCGCTAGGTCGTGGTGCTGCACGCCTGACCGGCAGGCCCGGCGCCGTGGTGCTCGAAGTTGCCAATCAGGG
CCGCTACGAAGCCACCTCGCCAGAATCACTGCTGCAGGATCAACTGGGCTGGAAACTGCCCGTGTCGCACCTTGTATGGT
GGGTACGCGGTCTACCGGCACCGGACAGCAAAAGCAACGTGACACTCGATGGCGACAGCCGACTGGCCAGCCTTGAACAG
GATGGCTGGCAGGTGGAATACCTCAGCTACGTCGAGCAGAACGGCTATTGGCTGCCAGAGCGCGTCAAGCTGCACGGGCA
GGATCTGGATGTCACGCTGGTCATCAAGGACTGGCAGCCTCGCAAACTGGGGCAATGA

Upstream 100 bases:

>100_bases
GCCAAAAAAGTCTGGGCCAAGGCCCTTGAACAACAACCCGACAGCACCGTCCTGCGCAGCACATTGCGACGCTTGACCGG
ATCAGAGACCCTTTGACGTT

Downstream 100 bases:

>100_bases
GCATGAGCACGCCACAACTGGTTTTGCCTGCCCCGGCAAAACTCAACCTCATGCTGCACATTCTCGGACGTCGTCCTGAC
GGTTATCACGAACTGCAGAC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MFLRHVIVFSLIALLTGCAGLTSREAVQGKGDPAQWREHKQQLSSLDGWQINGKVGIRAPKDSGSGTLFWLQRQDYYDIR
LSGPLGRGAARLTGRPGAVVLEVANQGRYEATSPESLLQDQLGWKLPVSHLVWWVRGLPAPDSKSNVTLDGDSRLASLEQ
DGWQVEYLSYVEQNGYWLPERVKLHGQDLDVTLVIKDWQPRKLGQ

Sequences:

>Translated_205_residues
MFLRHVIVFSLIALLTGCAGLTSREAVQGKGDPAQWREHKQQLSSLDGWQINGKVGIRAPKDSGSGTLFWLQRQDYYDIR
LSGPLGRGAARLTGRPGAVVLEVANQGRYEATSPESLLQDQLGWKLPVSHLVWWVRGLPAPDSKSNVTLDGDSRLASLEQ
DGWQVEYLSYVEQNGYWLPERVKLHGQDLDVTLVIKDWQPRKLGQ
>Mature_205_residues
MFLRHVIVFSLIALLTGCAGLTSREAVQGKGDPAQWREHKQQLSSLDGWQINGKVGIRAPKDSGSGTLFWLQRQDYYDIR
LSGPLGRGAARLTGRPGAVVLEVANQGRYEATSPESLLQDQLGWKLPVSHLVWWVRGLPAPDSKSNVTLDGDSRLASLEQ
DGWQVEYLSYVEQNGYWLPERVKLHGQDLDVTLVIKDWQPRKLGQ

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=202, Percent_Identity=28.2178217821782, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 22929; Mature: 22929

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRHVIVFSLIALLTGCAGLTSREAVQGKGDPAQWREHKQQLSSLDGWQINGKVGIRAP
CHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEECCEEEEECC
KDSGSGTLFWLQRQDYYDIRLSGPLGRGAARLTGRPGAVVLEVANQGRYEATSPESLLQD
CCCCCEEEEEEECCCEEEEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHH
QLGWKLPVSHLVWWVRGLPAPDSKSNVTLDGDSRLASLEQDGWQVEYLSYVEQNGYWLPE
HCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCCEEHHHHHHHCCCCCCCH
RVKLHGQDLDVTLVIKDWQPRKLGQ
HEEECCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MFLRHVIVFSLIALLTGCAGLTSREAVQGKGDPAQWREHKQQLSSLDGWQINGKVGIRAP
CHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEEECCEEEEECC
KDSGSGTLFWLQRQDYYDIRLSGPLGRGAARLTGRPGAVVLEVANQGRYEATSPESLLQD
CCCCCEEEEEEECCCEEEEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHH
QLGWKLPVSHLVWWVRGLPAPDSKSNVTLDGDSRLASLEQDGWQVEYLSYVEQNGYWLPE
HCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCCEEHHHHHHHCCCCCCCH
RVKLHGQDLDVTLVIKDWQPRKLGQ
HEEECCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA