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The map label for this gene is dut [H]

Identifier: 66043490

GI number: 66043490

Start: 234646

End: 235101

Strand: Reverse

Name: dut [H]

Synonym: Psyr_0220

Alternate gene names: 66043490

Gene position: 235101-234646 (Counterclockwise)

Preceding gene: 66043491

Following gene: 66043489

Centisome position: 3.86

GC content: 62.5

Gene sequence:

>456_bases
ATGCACGCTCTACAAGCCAAGATCCTCGACCCTCGCATTGGCAACGAATTTCCGCTGCCGGCTTACGCCACTACCGGCTC
GGCTGGCCTGGACCTGCGCGCCATGCTCAAGGAAGACACGCTCCTTGAGCCGGGCCAGACCCTGCTGATCCCTACCGGCC
TGTCGATTTACATTGGCGATCCGGGTCTTGCCGCGCTGATCCTGCCGCGCTCCGGCCTGGGCCACAAACACGGCATCGTG
CTGGGCAATCTGGTAGGCCTGATCGACTCCGATTACCAGGGCGAACTGATGGTCTCCTGCTGGAACCGCGGCCACACGGC
TTTCAACATCGCCGTCGGCGAGCGCATCGCCCAATTGGTCCTGGTGCCGGTCGTACAGGCGCACTTCGAGCTGGTTGAAG
CGTTCGACGAAAGTCAGCGTGGCGCTGGCGGCTTCGGTCATTCAGGCAGTCATTGA

Upstream 100 bases:

>100_bases
AGGGTTGCACGCAACGCTTTTCGCCCAGACCAGCAAGGCCAAGATCGCTCGCCAGCTGGTCACTTTTATCGCCGACCGCA
TGAATCAGGTTTAACTACGA

Downstream 100 bases:

>100_bases
TCGCCATTCGGCACATAATCCGGGTTCGGAACAAGGCTCAGGGAGAGGTCGGTGAAAGGTAGTCAGCACAGCGCTTCACA
GCACGCCATACCCACCGACA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MHALQAKILDPRIGNEFPLPAYATTGSAGLDLRAMLKEDTLLEPGQTLLIPTGLSIYIGDPGLAALILPRSGLGHKHGIV
LGNLVGLIDSDYQGELMVSCWNRGHTAFNIAVGERIAQLVLVPVVQAHFELVEAFDESQRGAGGFGHSGSH

Sequences:

>Translated_151_residues
MHALQAKILDPRIGNEFPLPAYATTGSAGLDLRAMLKEDTLLEPGQTLLIPTGLSIYIGDPGLAALILPRSGLGHKHGIV
LGNLVGLIDSDYQGELMVSCWNRGHTAFNIAVGERIAQLVLVPVVQAHFELVEAFDESQRGAGGFGHSGSH
>Mature_151_residues
MHALQAKILDPRIGNEFPLPAYATTGSAGLDLRAMLKEDTLLEPGQTLLIPTGLSIYIGDPGLAALILPRSGLGHKHGIV
LGNLVGLIDSDYQGELMVSCWNRGHTAFNIAVGERIAQLVLVPVVQAHFELVEAFDESQRGAGGFGHSGSH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=33.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=33.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=33.5714285714286, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=69.5364238410596, Blast_Score=223, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI71988561, Length=130, Percent_Identity=40, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=153, Percent_Identity=35.9477124183007, Blast_Score=77, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=30, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583610, Length=130, Percent_Identity=30, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16002; Mature: 16002

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHALQAKILDPRIGNEFPLPAYATTGSAGLDLRAMLKEDTLLEPGQTLLIPTGLSIYIGD
CCCCCCEECCCCCCCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCEEEEECCCEEEECC
PGLAALILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSCWNRGHTAFNIAVGERIAQLV
CCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHH
LVPVVQAHFELVEAFDESQRGAGGFGHSGSH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHALQAKILDPRIGNEFPLPAYATTGSAGLDLRAMLKEDTLLEPGQTLLIPTGLSIYIGD
CCCCCCEECCCCCCCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCEEEEECCCEEEECC
PGLAALILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSCWNRGHTAFNIAVGERIAQLV
CCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHH
LVPVVQAHFELVEAFDESQRGAGGFGHSGSH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA