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The map label for this gene is gcvA [H]

Identifier: 66043343

GI number: 66043343

Start: 75159

End: 76079

Strand: Reverse

Name: gcvA [H]

Synonym: Psyr_0072

Alternate gene names: 66043343

Gene position: 76079-75159 (Counterclockwise)

Preceding gene: 66043345

Following gene: 66043342

Centisome position: 1.25

GC content: 61.13

Gene sequence:

>921_bases
ATGCCTCGCAGTCTCCCTCCTCTTTACGCACTAAGGGCTTTTGAAGCAGCTGCGCGGCATACCTCGTTTACCCGAGCGGC
CGAGGAACTTTCGATCACCCAAAGTGCAGTAAGCCGGCATATTCGTACCCTGGAAGAGCACTTTGCCTGCCGCTTGTTTC
AGCGCAACGGGCGTAACTTGCAGCTCACCGAGTCGGCACGCGCGCTGTTGCCCGGCGTGCGCGACGGGTTCCTGGCACTG
GAACGCGCTTGCAGCACCTTGCAGACCGAAGAAGGTATTCTGCGCATGAAAGCCCCGTCGACCCTGACCATGCGCTGGCT
GCTCGCGCGGCTCAGCCGTTTTCGTCATCTGCAGGTGGGCAACGAGGTGCAACTGACCAGCGCGTGGATGGAAATCGATG
CGGTGGACTTTACCCAGGAGCCTTTCGATTGCGCGATTCTACTCAGCCAGGGGCATTTCCCGTCGGACTGGGAAGCCACA
CTGCTGTTTCCCGAATTGCTGATTCCGGTCGGTGCGCCCACCTTGTTGCAGGACCCGCCATGGGACGTGGAACGGCTGGC
AAAGGCTGAATTGCTGCATCCCACCCCTGATCGCCGTGACTGGCGCACCTGGTTGAACCGCATGGGTCTGGCCGATCAGG
TGTCGCTCAAAGGCGGGCAGGTGTTCGACACCCTGGAGCTGGGAATGATTGCCGCAGCACGCGGCTACGGTGTGTCGATG
GGCGACCTGCTGATGGTCGCCGAGGACGTGGCGCAGGCGCGTCTGAGCCTGCCGTGGCGCACGGCGGTGTTCAGTGGCGA
AAACTATTATCTGGTCTGGCCGCGTACGAGGCCCGGCGCAGAGCGGTTGCGACGCCTCAGCGATTTTCTTCAGGCGGAGG
TGGGTGCCATGAAGTTGCCGTTAGTTGAAGTACTCTATTAA

Upstream 100 bases:

>100_bases
GCTAAACACAAAACGATAAATCCGATGCTCAGCATGACTAAAACTCATCTGAAGCCATTTGCAGTAAAGTGGCTGCTCAT
CGTCCGCAGGAAGCGCCCTC

Downstream 100 bases:

>100_bases
AATTTAGTTTAATATTGAACTGCCATCATCTGTAAATGTCTCGCTCCGCTCTCAAGTAACGACCTTGATGGCCGAAGCAT
TGCTTGAAGATTGCTCGTTC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPRSLPPLYALRAFEAAARHTSFTRAAEELSITQSAVSRHIRTLEEHFACRLFQRNGRNLQLTESARALLPGVRDGFLAL
ERACSTLQTEEGILRMKAPSTLTMRWLLARLSRFRHLQVGNEVQLTSAWMEIDAVDFTQEPFDCAILLSQGHFPSDWEAT
LLFPELLIPVGAPTLLQDPPWDVERLAKAELLHPTPDRRDWRTWLNRMGLADQVSLKGGQVFDTLELGMIAAARGYGVSM
GDLLMVAEDVAQARLSLPWRTAVFSGENYYLVWPRTRPGAERLRRLSDFLQAEVGAMKLPLVEVLY

Sequences:

>Translated_306_residues
MPRSLPPLYALRAFEAAARHTSFTRAAEELSITQSAVSRHIRTLEEHFACRLFQRNGRNLQLTESARALLPGVRDGFLAL
ERACSTLQTEEGILRMKAPSTLTMRWLLARLSRFRHLQVGNEVQLTSAWMEIDAVDFTQEPFDCAILLSQGHFPSDWEAT
LLFPELLIPVGAPTLLQDPPWDVERLAKAELLHPTPDRRDWRTWLNRMGLADQVSLKGGQVFDTLELGMIAAARGYGVSM
GDLLMVAEDVAQARLSLPWRTAVFSGENYYLVWPRTRPGAERLRRLSDFLQAEVGAMKLPLVEVLY
>Mature_305_residues
PRSLPPLYALRAFEAAARHTSFTRAAEELSITQSAVSRHIRTLEEHFACRLFQRNGRNLQLTESARALLPGVRDGFLALE
RACSTLQTEEGILRMKAPSTLTMRWLLARLSRFRHLQVGNEVQLTSAWMEIDAVDFTQEPFDCAILLSQGHFPSDWEATL
LFPELLIPVGAPTLLQDPPWDVERLAKAELLHPTPDRRDWRTWLNRMGLADQVSLKGGQVFDTLELGMIAAARGYGVSMG
DLLMVAEDVAQARLSLPWRTAVFSGENYYLVWPRTRPGAERLRRLSDFLQAEVGAMKLPLVEVLY

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=276, Percent_Identity=35.5072463768116, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786448, Length=280, Percent_Identity=30.7142857142857, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=28.5223367697594, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI145693193, Length=153, Percent_Identity=30.718954248366, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=278, Percent_Identity=26.6187050359712, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787128, Length=140, Percent_Identity=32.1428571428571, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34596; Mature: 34465

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSLPPLYALRAFEAAARHTSFTRAAEELSITQSAVSRHIRTLEEHFACRLFQRNGRNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QLTESARALLPGVRDGFLALERACSTLQTEEGILRMKAPSTLTMRWLLARLSRFRHLQVG
EEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
NEVQLTSAWMEIDAVDFTQEPFDCAILLSQGHFPSDWEATLLFPELLIPVGAPTLLQDPP
CEEEEEHHHHEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCHHCCCCC
WDVERLAKAELLHPTPDRRDWRTWLNRMGLADQVSLKGGQVFDTLELGMIAAARGYGVSM
CCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHEECCCCCCHHHHHHHHHHHHCCCCCCH
GDLLMVAEDVAQARLSLPWRTAVFSGENYYLVWPRTRPGAERLRRLSDFLQAEVGAMKLP
HHHHHHHHHHHHHHHCCCHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHCC
LVEVLY
HHHHCC
>Mature Secondary Structure 
PRSLPPLYALRAFEAAARHTSFTRAAEELSITQSAVSRHIRTLEEHFACRLFQRNGRNL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QLTESARALLPGVRDGFLALERACSTLQTEEGILRMKAPSTLTMRWLLARLSRFRHLQVG
EEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
NEVQLTSAWMEIDAVDFTQEPFDCAILLSQGHFPSDWEATLLFPELLIPVGAPTLLQDPP
CEEEEEHHHHEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCHHCCCCC
WDVERLAKAELLHPTPDRRDWRTWLNRMGLADQVSLKGGQVFDTLELGMIAAARGYGVSM
CCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHEECCCCCCHHHHHHHHHHHHCCCCCCH
GDLLMVAEDVAQARLSLPWRTAVFSGENYYLVWPRTRPGAERLRRLSDFLQAEVGAMKLP
HHHHHHHHHHHHHHHCCCHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHCC
LVEVLY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]