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The map label for this gene is rhtB [H]

Identifier: 66043321

GI number: 66043321

Start: 51165

End: 51797

Strand: Reverse

Name: rhtB [H]

Synonym: Psyr_0050

Alternate gene names: 66043321

Gene position: 51797-51165 (Counterclockwise)

Preceding gene: 66043322

Following gene: 66043320

Centisome position: 0.85

GC content: 61.61

Gene sequence:

>633_bases
ATGGCGCTTGAAACGTGGCTGGGCTTTTTTGCGGCCTGCTGGATTATCAGTCTGTCTCCTGGCGCGGGCGCCATCGCTTC
GATGTCCTGCGGCCTGCAATACGGTTTCTGGCGTGGCTACTGGAATGCCCTGGGCTTGCAGATTGCGTTGGTGGCACAGA
TCGCGATTGTCGCGGCAGGTCTGGGTGCCGTGCTGGCGGCCTCCGATATGGCGTTCACACTGATCAAGTGGTTCGGCGTC
GCATACCTGGTCTATCTCGGCATCAAGCAATGGCGCGCCCTGCCAGCCGACCTGGCCGACGAATCTGCCGTGCGCCCTGT
GGGCAAACCGATGACGCTGGTATTGCGTGGTTTTCTGGTGAATATCAGCAACCCCAAGGCGCTGATCTTCATTCTGGCGA
TCCTGCCACAGTTCATCGACCCGGCCGCTCCGCTGCTCATCCAGTACGCGATCATCGCGGCGACCATGGTGGTGGTCGAT
CTGATCGTGATGGCGGGCTACACCGGGCTGGCATCCAAGGTGTTGCGCCTGCTCAAGACGCCTCGCCAGCAACGTCGCAT
GAATCGCACCTTCGCCAGCCTGTTCGTGGGCGCGGCAGGTTTCCTCGCGACGTTGCATCGCGGTACGGCCTGA

Upstream 100 bases:

>100_bases
GTGGTCGCAAAGCAGCCGGACGAGCAGGATTAGAAATCGTTAGCTTTGCAAAGCAACCCGCATTAATTTACTGAGCATTG
GACTTTGCTTGAGGTGCACG

Downstream 100 bases:

>100_bases
AACGTGTCGGTCTCGAGCCCTGAGCAGACACCCGTTTTGCTCAGGGCGACTGAAGGCTGAACGTAAGATTCTGTAACAAA
AATAGACCTCGACTATTGAG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MALETWLGFFAACWIISLSPGAGAIASMSCGLQYGFWRGYWNALGLQIALVAQIAIVAAGLGAVLAASDMAFTLIKWFGV
AYLVYLGIKQWRALPADLADESAVRPVGKPMTLVLRGFLVNISNPKALIFILAILPQFIDPAAPLLIQYAIIAATMVVVD
LIVMAGYTGLASKVLRLLKTPRQQRRMNRTFASLFVGAAGFLATLHRGTA

Sequences:

>Translated_210_residues
MALETWLGFFAACWIISLSPGAGAIASMSCGLQYGFWRGYWNALGLQIALVAQIAIVAAGLGAVLAASDMAFTLIKWFGV
AYLVYLGIKQWRALPADLADESAVRPVGKPMTLVLRGFLVNISNPKALIFILAILPQFIDPAAPLLIQYAIIAATMVVVD
LIVMAGYTGLASKVLRLLKTPRQQRRMNRTFASLFVGAAGFLATLHRGTA
>Mature_209_residues
ALETWLGFFAACWIISLSPGAGAIASMSCGLQYGFWRGYWNALGLQIALVAQIAIVAAGLGAVLAASDMAFTLIKWFGVA
YLVYLGIKQWRALPADLADESAVRPVGKPMTLVLRGFLVNISNPKALIFILAILPQFIDPAAPLLIQYAIIAATMVVVDL
IVMAGYTGLASKVLRLLKTPRQQRRMNRTFASLFVGAAGFLATLHRGTA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=204, Percent_Identity=40.1960784313725, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788099, Length=208, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22529; Mature: 22398

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALETWLGFFAACWIISLSPGAGAIASMSCGLQYGFWRGYWNALGLQIALVAQIAIVAAG
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAVLAASDMAFTLIKWFGVAYLVYLGIKQWRALPADLADESAVRPVGKPMTLVLRGFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHH
NISNPKALIFILAILPQFIDPAAPLLIQYAIIAATMVVVDLIVMAGYTGLASKVLRLLKT
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
PRQQRRMNRTFASLFVGAAGFLATLHRGTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALETWLGFFAACWIISLSPGAGAIASMSCGLQYGFWRGYWNALGLQIALVAQIAIVAAG
CHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAVLAASDMAFTLIKWFGVAYLVYLGIKQWRALPADLADESAVRPVGKPMTLVLRGFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHH
NISNPKALIFILAILPQFIDPAAPLLIQYAIIAATMVVVDLIVMAGYTGLASKVLRLLKT
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
PRQQRRMNRTFASLFVGAAGFLATLHRGTA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]