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The map label for this gene is ampR [H]

Identifier: 66043301

GI number: 66043301

Start: 34350

End: 35300

Strand: Direct

Name: ampR [H]

Synonym: Psyr_0030

Alternate gene names: 66043301

Gene position: 34350-35300 (Clockwise)

Preceding gene: 66043296

Following gene: 66043302

Centisome position: 0.56

GC content: 62.99

Gene sequence:

>951_bases
ATGTTTGAATCTTTCGGCGATGTGTCACTCGACCTGCTGCGTGCCTTTGAGGTCGCGGCGCGCTTACGCAGCTTCACCGC
GGCAGCCCTCGAACTGGGCACGACACAGCCCGCTGTCAGCCAGCAGATCAAGCGCCTCGAAGAGCAACTCGCTACCCGTC
TGTTTGACCGCATCTACCGTGGTATCGAATTGACCGACGCCGGTGAAGTGTTGTTCAGCCATGTGCAGGCCGGGCTGCAA
TCCATCGACAGCGGAGTGATAGCCATCACCGAACACCACCAGCACGAAGTGCTTCAAGTGGCGACCGACTTCGCCTTCGC
CGCTTACTGGCTGATGCCGCGTCTGCACCGCTTCCACAAGGTCAACCCGGACGTGGACGTCAGCCTGGTCACCAGCGAGC
GTACCCACAGCATGTTACGCGCCGATATCGACGTTGCCGTGCTGTTCGGTGACGGGCGCTTCAAACAGGGAGAAAGCCAC
TGGCTGTTCAGCGAAGAAGTCTTCCCGGTGTGCAGCCCGCAACTGCTCGCCGGGCGCCAGACGCCGCTGCCCACCGATGC
GCTGCGCGACTTTCCACTCCTGCACCTGCGTGGCGAAAGCATCAACAACTGGTTCGACTGGGCGGGTGTATTCCGCGCGC
TGGACATCCCCCAGGCCCCCGCGCCCGGTCAACTGCGCTTCGACAACTACACCCTGCTGATCCAGGCGGCCATCGGCGGA
CAAGGCATCGCCATCGGCTGGAAGCACCTGGTCGACGACCTCCTCGATCAGGGCCTGCTCTGTCGCCCTATCGCCGGCGC
GGCGATTTCCGGTCAGGGCTATTACGTGGTCCTGCCCCAGCGCAAACGCCGCGTGCAGATCGTTCAGCAGTTCGTGGACT
GGCTGGCCAGCGAGCAGGCGTTGAGCGGGGTTTCGCTGACGGGCAGGCCATTGCCTTCGATTGCGGTGTAA

Upstream 100 bases:

>100_bases
CCATTGTGAAAAGTTATGCGAAAGTGCCGGGTATGAGGATGGAGTCCATAAGTTTCAGGGTAAAGCCCATACACAGCAAT
GCCTAGGATAAGCCCAGCTA

Downstream 100 bases:

>100_bases
TATTGCCGCGCTGAAAAGTGACCACTTGGTCCGTCGCAGCGCACACATCAACAAAATGGTCACGTATACGCTTCAACCTC
ATGAGCTGTCTGCCATGGGC

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MFESFGDVSLDLLRAFEVAARLRSFTAAALELGTTQPAVSQQIKRLEEQLATRLFDRIYRGIELTDAGEVLFSHVQAGLQ
SIDSGVIAITEHHQHEVLQVATDFAFAAYWLMPRLHRFHKVNPDVDVSLVTSERTHSMLRADIDVAVLFGDGRFKQGESH
WLFSEEVFPVCSPQLLAGRQTPLPTDALRDFPLLHLRGESINNWFDWAGVFRALDIPQAPAPGQLRFDNYTLLIQAAIGG
QGIAIGWKHLVDDLLDQGLLCRPIAGAAISGQGYYVVLPQRKRRVQIVQQFVDWLASEQALSGVSLTGRPLPSIAV

Sequences:

>Translated_316_residues
MFESFGDVSLDLLRAFEVAARLRSFTAAALELGTTQPAVSQQIKRLEEQLATRLFDRIYRGIELTDAGEVLFSHVQAGLQ
SIDSGVIAITEHHQHEVLQVATDFAFAAYWLMPRLHRFHKVNPDVDVSLVTSERTHSMLRADIDVAVLFGDGRFKQGESH
WLFSEEVFPVCSPQLLAGRQTPLPTDALRDFPLLHLRGESINNWFDWAGVFRALDIPQAPAPGQLRFDNYTLLIQAAIGG
QGIAIGWKHLVDDLLDQGLLCRPIAGAAISGQGYYVVLPQRKRRVQIVQQFVDWLASEQALSGVSLTGRPLPSIAV
>Mature_316_residues
MFESFGDVSLDLLRAFEVAARLRSFTAAALELGTTQPAVSQQIKRLEEQLATRLFDRIYRGIELTDAGEVLFSHVQAGLQ
SIDSGVIAITEHHQHEVLQVATDFAFAAYWLMPRLHRFHKVNPDVDVSLVTSERTHSMLRADIDVAVLFGDGRFKQGESH
WLFSEEVFPVCSPQLLAGRQTPLPTDALRDFPLLHLRGESINNWFDWAGVFRALDIPQAPAPGQLRFDNYTLLIQAAIGG
QGIAIGWKHLVDDLLDQGLLCRPIAGAAISGQGYYVVLPQRKRRVQIVQQFVDWLASEQALSGVSLTGRPLPSIAV

Specific function: This protein is a positive regulator of gene expression of cephalosporinase (AmpC) [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=32.7814569536424, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=31.8493150684932, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788706, Length=291, Percent_Identity=30.5841924398625, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=26.5993265993266, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=26.7605633802817, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=252, Percent_Identity=29.7619047619048, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790262, Length=279, Percent_Identity=28.3154121863799, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787601, Length=128, Percent_Identity=33.59375, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367136, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788297, Length=167, Percent_Identity=29.940119760479, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35104; Mature: 35104

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFESFGDVSLDLLRAFEVAARLRSFTAAALELGTTQPAVSQQIKRLEEQLATRLFDRIYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GIELTDAGEVLFSHVQAGLQSIDSGVIAITEHHQHEVLQVATDFAFAAYWLMPRLHRFHK
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VNPDVDVSLVTSERTHSMLRADIDVAVLFGDGRFKQGESHWLFSEEVFPVCSPQLLAGRQ
CCCCCEEEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHCCCC
TPLPTDALRDFPLLHLRGESINNWFDWAGVFRALDIPQAPAPGQLRFDNYTLLIQAAIGG
CCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEEEEECC
QGIAIGWKHLVDDLLDQGLLCRPIAGAAISGQGYYVVLPQRKRRVQIVQQFVDWLASEQA
CEEEECHHHHHHHHHCCCCEEECCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
LSGVSLTGRPLPSIAV
HCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFESFGDVSLDLLRAFEVAARLRSFTAAALELGTTQPAVSQQIKRLEEQLATRLFDRIYR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GIELTDAGEVLFSHVQAGLQSIDSGVIAITEHHQHEVLQVATDFAFAAYWLMPRLHRFHK
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VNPDVDVSLVTSERTHSMLRADIDVAVLFGDGRFKQGESHWLFSEEVFPVCSPQLLAGRQ
CCCCCEEEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHCCCC
TPLPTDALRDFPLLHLRGESINNWFDWAGVFRALDIPQAPAPGQLRFDNYTLLIQAAIGG
CCCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEEEEECC
QGIAIGWKHLVDDLLDQGLLCRPIAGAAISGQGYYVVLPQRKRRVQIVQQFVDWLASEQA
CEEEECHHHHHHHHHCCCCEEECCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
LSGVSLTGRPLPSIAV
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2788410 [H]