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The map label for this gene is hemF [H]

Identifier: 66043295

GI number: 66043295

Start: 27721

End: 28635

Strand: Direct

Name: hemF [H]

Synonym: Psyr_0024

Alternate gene names: 66043295

Gene position: 27721-28635 (Clockwise)

Preceding gene: 66043293

Following gene: 66043296

Centisome position: 0.45

GC content: 61.31

Gene sequence:

>915_bases
ATGAGTACCCGTACCGAGGCCGTAAAAGCCTACCTGCTCGACCTGCAAGACCGTATCTGCACTGCGCTTGAACAGGAAGA
TGGCAGCGCACACTTCATGGAAGATGCCTGGACGCGTCCGGCGGGCGGCGGTGGTCGCACGCGGGTGATCGAGAATGGCA
CGGTCATCGAGAAAGGCGGTGTCAATTTTTCCCATGTTTTCGGCAGCAATCTGCCGCCGTCTGCCAGCGCTCATCGGCCT
GAACTGGCCGGGCGTGGTTTCGAGGCGCTGGGCGTTTCGCTGGTGATCCATCCGCACAACCCGCATGTGCCGACGTCCCA
CGCCAACGTGCGCTTCTTCATTGCCGAGAAAGAAGGTGAAGAGGCCGTCTGGTGGTTCGGTGGCGGTTTCGACCTGACGC
CCTATTACGGCGTCGAGGAAGACTGCGTGCACTGGCACCGGGTTGCCGAGCGCGCCTGTGCGCCCTTCGGTGAGGATGTC
TACCCGCGCTACAAGGCCTGGTGCGACAGTTACTTCCACCTCAAGCATCGCGATGAACCGCGCGGCATCGGTGGTCTGTT
TTTCGATGACGTGAACCAGTGGGATTTCGACACCAGCTTCGCCTTCATCCGCGCCATCGGTGATGCGTTCATCAATGCCT
ATCTGCCGATCGTGCGGCGGCGCAAGGCGGCGGCCTATACCGTGCAGCAGCGTGAGTTTCAGGAATTCCGTCGTGGTCGC
TACGTCGAGTTCAACCTGGTGTATGACCGCGGCACGTTGTTCGGTCTGCAATCGGGTGGGCGTACCGAATCGATCCTCAT
GTCGCTGCCGCCGCAAGTGCGCTGGGGTTATGACTGGAAGGCCGCGCCGGGCAGCGAAGAAGCGCGTCTGACCGAATACT
TCCTGACCGACCGCGACTGGCTGGCGGAAAACTGA

Upstream 100 bases:

>100_bases
CTGCCAGCCCGCGCCGCCGATGGTATGCTACGCGCCAATTTGCCTGCGCCTCTTTTGCTGCGGGGGCTCAGCCGTTTGAC
GTCAGCCGTAAGGGATCCTC

Downstream 100 bases:

>100_bases
TGGACCGGTACGTCGTCTTCGGTAATCCCATCGGCCACAGCAAGTCGCCGTTGATTCATCGCCTGTTTGCCGAGCAAACC
GGGCAAACGCTGGATTACCA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSTRTEAVKAYLLDLQDRICTALEQEDGSAHFMEDAWTRPAGGGGRTRVIENGTVIEKGGVNFSHVFGSNLPPSASAHRP
ELAGRGFEALGVSLVIHPHNPHVPTSHANVRFFIAEKEGEEAVWWFGGGFDLTPYYGVEEDCVHWHRVAERACAPFGEDV
YPRYKAWCDSYFHLKHRDEPRGIGGLFFDDVNQWDFDTSFAFIRAIGDAFINAYLPIVRRRKAAAYTVQQREFQEFRRGR
YVEFNLVYDRGTLFGLQSGGRTESILMSLPPQVRWGYDWKAAPGSEEARLTEYFLTDRDWLAEN

Sequences:

>Translated_304_residues
MSTRTEAVKAYLLDLQDRICTALEQEDGSAHFMEDAWTRPAGGGGRTRVIENGTVIEKGGVNFSHVFGSNLPPSASAHRP
ELAGRGFEALGVSLVIHPHNPHVPTSHANVRFFIAEKEGEEAVWWFGGGFDLTPYYGVEEDCVHWHRVAERACAPFGEDV
YPRYKAWCDSYFHLKHRDEPRGIGGLFFDDVNQWDFDTSFAFIRAIGDAFINAYLPIVRRRKAAAYTVQQREFQEFRRGR
YVEFNLVYDRGTLFGLQSGGRTESILMSLPPQVRWGYDWKAAPGSEEARLTEYFLTDRDWLAEN
>Mature_303_residues
STRTEAVKAYLLDLQDRICTALEQEDGSAHFMEDAWTRPAGGGGRTRVIENGTVIEKGGVNFSHVFGSNLPPSASAHRPE
LAGRGFEALGVSLVIHPHNPHVPTSHANVRFFIAEKEGEEAVWWFGGGFDLTPYYGVEEDCVHWHRVAERACAPFGEDVY
PRYKAWCDSYFHLKHRDEPRGIGGLFFDDVNQWDFDTSFAFIRAIGDAFINAYLPIVRRRKAAAYTVQQREFQEFRRGRY
VEFNLVYDRGTLFGLQSGGRTESILMSLPPQVRWGYDWKAAPGSEEARLTEYFLTDRDWLAEN

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=313, Percent_Identity=45.3674121405751, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1788777, Length=301, Percent_Identity=68.1063122923588, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320249, Length=315, Percent_Identity=45.0793650793651, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17864388, Length=303, Percent_Identity=47.5247524752475, Blast_Score=278, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 34594; Mature: 34463

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRTEAVKAYLLDLQDRICTALEQEDGSAHFMEDAWTRPAGGGGRTRVIENGTVIEKGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCCEEEEECCEEEECCC
VNFSHVFGSNLPPSASAHRPELAGRGFEALGVSLVIHPHNPHVPTSHANVRFFIAEKEGE
CCCHHHCCCCCCCCCCCCCCHHHCCCCCEECEEEEEECCCCCCCCCCCCEEEEEEECCCC
EAVWWFGGGFDLTPYYGVEEDCVHWHRVAERACAPFGEDVYPRYKAWCDSYFHLKHRDEP
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHEEECCCCCC
RGIGGLFFDDVNQWDFDTSFAFIRAIGDAFINAYLPIVRRRKAAAYTVQQREFQEFRRGR
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YVEFNLVYDRGTLFGLQSGGRTESILMSLPPQVRWGYDWKAAPGSEEARLTEYFLTDRDW
EEEEEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHH
LAEN
HCCC
>Mature Secondary Structure 
STRTEAVKAYLLDLQDRICTALEQEDGSAHFMEDAWTRPAGGGGRTRVIENGTVIEKGG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCCEEEEECCEEEECCC
VNFSHVFGSNLPPSASAHRPELAGRGFEALGVSLVIHPHNPHVPTSHANVRFFIAEKEGE
CCCHHHCCCCCCCCCCCCCCHHHCCCCCEECEEEEEECCCCCCCCCCCCEEEEEEECCCC
EAVWWFGGGFDLTPYYGVEEDCVHWHRVAERACAPFGEDVYPRYKAWCDSYFHLKHRDEP
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHEEECCCCCC
RGIGGLFFDDVNQWDFDTSFAFIRAIGDAFINAYLPIVRRRKAAAYTVQQREFQEFRRGR
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YVEFNLVYDRGTLFGLQSGGRTESILMSLPPQVRWGYDWKAAPGSEEARLTEYFLTDRDW
EEEEEEEEECCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHH
LAEN
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA