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The map label for this gene is gyrB [H]

Identifier: 66043275

GI number: 66043275

Start: 3809

End: 6226

Strand: Direct

Name: gyrB [H]

Synonym: Psyr_0004

Alternate gene names: 66043275

Gene position: 3809-6226 (Clockwise)

Preceding gene: 66043274

Following gene: 66043279

Centisome position: 0.06

GC content: 56.53

Gene sequence:

>2418_bases
ATGAGCGAAAACCAAACGTACGACTCGTCCAGCATTAAAGTGCTGAAAGGACTTGATGCCGTACGCAAGCGTCCCGGAAT
GTACATCGGTGATACAGACGATGGCAGCGGTCTGCACCACATGGTGTTCGAGGTGGTTGATAACTCGATCGACGAAGCAT
TGGCAGGCCATTGCGACGACATCAGCATCATCATCCACCCAGACGAATCTATCACCGTGCGCGACAACGGCCGCGGTATT
CCGGTAGACGTGCATAAAGAAGAAGGCGTGTCGGCAGCCGAGGTCATCATGACCGTGCTCCACGCTGGCGGTAAGTTCGA
TGACAACTCCTACAAAGTATCCGGTGGTTTGCACGGCGTAGGTGTCTCTGTAGTTAACGCCCTTTCAGAGCTTCTGTTGC
TGACGGTTCGTCGTAGCGGCAAGATCTGGGAACAGACCTATGTTCATGGTGTTCCACAGGAACCGATGAAGATCGTCGGT
GAAAGTGACAGCACAGGCACTCAGATCCACTTCAAGCCTTCAGCTGAAACGTTCAAGAACATCCACTTCAGCTGGGACAT
TCTGGCCAAGCGGATTCGTGAACTGTCTTTCCTGAACTCCGGTGTCGGCATTGTCCTCAAGGATGAGCGCAGCGGTAAGG
AAGAATTGTTCAAATACGAAGGCGGTCTGCGCGCATTCGTTGAGTACCTGAACACCAATAAAACGCCGGTCAACGAAGTG
TTCCACTTCAACATCCAGCGTGATGACGGTATCGGTGTCGAGATTGCTCTGCAGTGGAACGATAGCTTCAACGAGAACCT
GCTGTGCTTCACCAACAACATTCCGCAGCGTGATGGCGGTACTCACCTGGTGGGCTTCCGCTCTGCGCTGACGCGTAACC
TGAACAACTACATCGAGCAGGAAGGTCTGGCCAAGAAGCACAAGGTCGCGACCACCGGCGATGACGCGCGTGAAGGTCTG
ACTGCGATCATTTCGGTCAAGGTGCCTGATCCTAAGTTCAGCTCGCAGACCAAGGACAAGCTGGTTTCGTCCGAAGTCAA
AACGGCTGTCGAACAGGAAATGGGCAAGTACTTCTCCGACTTCCTGTTGGAAAACCCGAACGAAGCCAAAGCGGTTGTCG
GCAAGATGATCGACGCAGCCCGTGCCCGCGAAGCTGCGCGCAAGGCGCGCGAGATGACCCGTCGTAAAGGCGCGCTGGAT
ATCGCTGGCCTGCCGGGCAAACTGGCGGACTGCCAGGAAAAAGACCCTGCCCTCTCCGAACTGTACCTGGTGGAAGGGGA
CTCTGCTGGCGGATCAGCCAAGCAGGGGCGTAATCGTAGAACTCAGGCCATCCTGCCGCTCAAGGGCAAGATCCTGAACG
TTGAAAAAGCGCGTTTCGACAAGATGATCTCTTCGCAGGAAGTGGGCACCTTGATCACTGCGCTGGGCTGCGGTATTGGC
CGTGAAGAGTACAACATCGACAAGTTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTCGACGGCTCGCACAT
CCGTACCCTGCTGCTGACCTTCTTCTTCCGTCAGTTGCCCGAGCTGATCGAGCGTGGCTACATCTACATCGCTCAGCCGC
CGCTGTACAAGGTCAAGAAAGGCAAGCAGGAGCAGTACATCAAGGACGACGAGGCCATGGAAGAATACATGACCCAGTCG
GCCCTTGAAGACGCCAGCCTGCACCTCAGCGAATCGGCGCCGGGTATCTCCGGTACCGCACTGGAGAAGCTGGTCAACGA
CTTCCGCATGGTCATGAAGACGCTCAAGCGCCTGTCGCGTCTGTACCCACAGGAACTGACCGAGCACTTCGTCTACCTGC
CGCCGATCACGCTCGAACAACTGTCGGATCACGAGGGCATGCAAGCCTGGCTGGCACTGTTCGACGCGCGTCTGCGCACC
GGCGAGAAGTCAGGTCTGGTCTACAAGGCCAGCCTGCGTGAAGACCGTGAACGTAACGTCTGGCTGCCAGAGGTCGAACT
GATCTCCCACGGCCTGTCGAACTACGTCACCTTCAACCGCGACTTCTTCGGCAGCAACGACTACAAGACCGTTACCGCTC
TGGGCGCGCAGATCAGCACGCTGCTGGAAGAAGGTGCCTACGTACAACGTGGCGAGCGCAAGAAGCCCGTCAACGAGTTC
AAGGAAGCCCTCGCCTGGTTGATGGCCGAAAGCACCAAGCGCCACACCATCCAGCGCTACAAAGGTCTGGGTGAGATGAA
CCCGGACCAGCTGTGGGAAACCACCATGGACCCGAGTGTGCGCCGCATGCTGAAAGTCACCATCGAAGATGCCATCGGCG
CTGACCAGATCTTCAACACCCTCATGGGTGATGCGGTCGAGCCTCGCCGTGACTTCATCGAGAGCAATGCGCTGGCCGTG
TCCAACCTGGACTTCTGA

Upstream 100 bases:

>100_bases
GAATTTTTGAGGGAAGGCTGGCAGACGGAAACGCCAGTCGCTTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCA
CGACCATCGGGAGTGATTGC

Downstream 100 bases:

>100_bases
TGAGCGTATGATCAAAACATCAACCCCGGCGAAAGCCGGGGTTTTTGTTTGTGCTCTAAAACAGTTTCGCCAGTCGCAGA
TTCAAGCGGGACTTTTCCCG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSENQTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDDISIIIHPDESITVRDNGRGI
PVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSELLLLTVRRSGKIWEQTYVHGVPQEPMKIVG
ESDSTGTQIHFKPSAETFKNIHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTPVNEV
FHFNIQRDDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQEGLAKKHKVATTGDDAREGL
TAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKAVVGKMIDAARAREAARKAREMTRRKGALD
IAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRRTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDEAMEEYMTQS
ALEDASLHLSESAPGISGTALEKLVNDFRMVMKTLKRLSRLYPQELTEHFVYLPPITLEQLSDHEGMQAWLALFDARLRT
GEKSGLVYKASLREDRERNVWLPEVELISHGLSNYVTFNRDFFGSNDYKTVTALGAQISTLLEEGAYVQRGERKKPVNEF
KEALAWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFIESNALAV
SNLDF

Sequences:

>Translated_805_residues
MSENQTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDDISIIIHPDESITVRDNGRGI
PVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSELLLLTVRRSGKIWEQTYVHGVPQEPMKIVG
ESDSTGTQIHFKPSAETFKNIHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTPVNEV
FHFNIQRDDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQEGLAKKHKVATTGDDAREGL
TAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKAVVGKMIDAARAREAARKAREMTRRKGALD
IAGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRRTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDEAMEEYMTQS
ALEDASLHLSESAPGISGTALEKLVNDFRMVMKTLKRLSRLYPQELTEHFVYLPPITLEQLSDHEGMQAWLALFDARLRT
GEKSGLVYKASLREDRERNVWLPEVELISHGLSNYVTFNRDFFGSNDYKTVTALGAQISTLLEEGAYVQRGERKKPVNEF
KEALAWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFIESNALAV
SNLDF
>Mature_804_residues
SENQTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDDISIIIHPDESITVRDNGRGIP
VDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSELLLLTVRRSGKIWEQTYVHGVPQEPMKIVGE
SDSTGTQIHFKPSAETFKNIHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTPVNEVF
HFNIQRDDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQEGLAKKHKVATTGDDAREGLT
AIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSDFLLENPNEAKAVVGKMIDAARAREAARKAREMTRRKGALDI
AGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRNRRTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIGR
EEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKKGKQEQYIKDDEAMEEYMTQSA
LEDASLHLSESAPGISGTALEKLVNDFRMVMKTLKRLSRLYPQELTEHFVYLPPITLEQLSDHEGMQAWLALFDARLRTG
EKSGLVYKASLREDRERNVWLPEVELISHGLSNYVTFNRDFFGSNDYKTVTALGAQISTLLEEGAYVQRGERKKPVNEFK
EALAWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNTLMGDAVEPRRDFIESNALAVS
NLDF

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=568, Percent_Identity=25.3521126760563, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=549, Percent_Identity=25.3187613843352, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI48994957, Length=804, Percent_Identity=67.2885572139303, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=559, Percent_Identity=40.0715563506261, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535065, Length=538, Percent_Identity=27.1375464684015, Blast_Score=149, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI212645845, Length=533, Percent_Identity=26.266416510319, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=596, Percent_Identity=25.8389261744966, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17136538, Length=557, Percent_Identity=26.032315978456, Blast_Score=143, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90091; Mature: 89960

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENQTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDD
CCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ISIIIHPDESITVRDNGRGIPVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGV
EEEEEECCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSELLLLTVRRSGKIWEQTYVHGVPQEPMKIVGESDSTGTQIHFKPSAETFKN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCCCCCEEEECCCHHHHHH
IHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTPVNEV
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCE
FHFNIQRDDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQ
EEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHH
EGLAKKHKVATTGDDAREGLTAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSD
HCCHHHHCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLENPNEAKAVVGKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPALSE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCE
LYLVEGDSAGGSAKQGRNRRTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
EEEEECCCCCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKK
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDDEAMEEYMTQSALEDASLHLSESAPGISGTALEKLVNDFRMVMKTLKRLSR
CCHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYPQELTEHFVYLPPITLEQLSDHEGMQAWLALFDARLRTGEKSGLVYKASLREDRERNV
HCHHHHHHCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
WLPEVELISHGLSNYVTFNRDFFGSNDYKTVTALGAQISTLLEEGAYVQRGERKKPVNEF
CCCHHHHHHCCHHHCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHH
KEALAWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
LMGDAVEPRRDFIESNALAVSNLDF
HHCCCCCHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
SENQTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVVDNSIDEALAGHCDD
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
ISIIIHPDESITVRDNGRGIPVDVHKEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGV
EEEEEECCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSELLLLTVRRSGKIWEQTYVHGVPQEPMKIVGESDSTGTQIHFKPSAETFKN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCCCCCEEEECCCHHHHHH
IHFSWDILAKRIRELSFLNSGVGIVLKDERSGKEELFKYEGGLRAFVEYLNTNKTPVNEV
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCE
FHFNIQRDDGIGVEIALQWNDSFNENLLCFTNNIPQRDGGTHLVGFRSALTRNLNNYIEQ
EEEEEEECCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHH
EGLAKKHKVATTGDDAREGLTAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKYFSD
HCCHHHHCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLLENPNEAKAVVGKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPALSE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCE
LYLVEGDSAGGSAKQGRNRRTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIG
EEEEECCCCCCCCCCCCCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
REEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYIYIAQPPLYKVKK
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDDEAMEEYMTQSALEDASLHLSESAPGISGTALEKLVNDFRMVMKTLKRLSR
CCHHHHCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYPQELTEHFVYLPPITLEQLSDHEGMQAWLALFDARLRTGEKSGLVYKASLREDRERNV
HCHHHHHHCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
WLPEVELISHGLSNYVTFNRDFFGSNDYKTVTALGAQISTLLEEGAYVQRGERKKPVNEF
CCCHHHHHHCCHHHCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHH
KEALAWLMAESTKRHTIQRYKGLGEMNPDQLWETTMDPSVRRMLKVTIEDAIGADQIFNT
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
LMGDAVEPRRDFIESNALAVSNLDF
HHCCCCCHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170947; 7793912; 2540413 [H]