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The map label for this gene is recF [H]

Identifier: 66043274

GI number: 66043274

Start: 2701

End: 3804

Strand: Direct

Name: recF [H]

Synonym: Psyr_0003

Alternate gene names: 66043274

Gene position: 2701-3804 (Clockwise)

Preceding gene: 66043273

Following gene: 66043275

Centisome position: 0.04

GC content: 57.07

Gene sequence:

>1104_bases
ATGTCTCTTAGTCGCGTCTCGGTCACCGGGGTGCGCAATCTGCACCCGGTGACCCTCTCCCCCTCCCCGCGTATCAATAT
CCTCTACGGCGCAAACGGCAGTGGCAAAACCAGCGTGCTGGAAGCTATCCATCTGCTGGGCATTGCTCGCTCGTTCCGCA
GCAGTCGCCTGCTGCCCGTCATTCAGTACGAGCAGCCTTCCTGCACCGTCTTCGGTCAGGTCGATCTTGCACAAGGTGGA
CACAGCAATCTGGGTGTTTCACGCGACCGCCAAGGCGAGTTCCAGATTCGTATCGATGGGCAGAACGCCAGAAGCGCTGC
GCAGCTTGCAGAAATTCTGCCGCTGCAGCTCATCAACCCCGACAGCTTCCGATTGCTTGAAGGTGCGCCGAAGATACGCA
GGCAGTTTCTGGATTGGGGAGTGTTCCACGTGGAACCTCGCTTTATGGCCACGTGGCAGCGCCTGCAGAAGGCCCTCAAG
CAGCGAAACTCGTGGCTTCGGCATGGTACACTTGACGCCGCTTCACAGGCGGCATGGGACCGTGAGCTGTGCTCGGCAAG
TGACGAGATCGACGAGTTCCGCCGGGCTTATATCAAGGCATTGAAGCCAGTCTTTGAACAGACTTTGAGCGAACTTGTTG
AGCTTGAAGGTTTGACCCTGAGCTACTACCGAGGATGGGACAAGGAAAAAGAGCTAAGTACAGTGCTCGCCTCTTCCATC
CACCGCGATCAGCAAATGGGCCACACCCAGGCCGGACCACAGCGAGCTGATCTGCGCCTTAGATTAGGCGCGCATAATGC
GGCAGATATTCTGTCTCGCGGTCAGCAAAAGCTGGTGGTCTGCGCACTGCGTATTGCTCAGGGACATCTGGTCAGCCAGG
TCCGTCGCGGCCAGTGTATTTACCTGGTAGATGATTTGCCCTCCGAACTGGATGACAACCATCGACGCGCGCTTTGCCGC
TTGCTGGAAGAATTACGCTGCCAGGTGTTTATCACCTGTGTAGATCAAGAATTTTTGAGGGAAGGCTGGCAGACGGAAAC
GCCAGTCGCTTTGTTCCACGTGGAACAAGGCCGTATCACCCAGACCCACGACCATCGGGAGTGA

Upstream 100 bases:

>100_bases
GACTCCAATAGCAGCGCCCTGGTGCAAGAGTCCGATAACGACGACTCCGCCTACGTTGTCATGCCGATGCGTCTGTAACT
TCATGTCCAGCGCAACCTAG

Downstream 100 bases:

>100_bases
TTGCATGAGCGAAAACCAAACGTACGACTCGTCCAGCATTAAAGTGCTGAAAGGACTTGATGCCGTACGCAAGCGTCCCG
GAATGTACATCGGTGATACA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSLSRVSVTGVRNLHPVTLSPSPRINILYGANGSGKTSVLEAIHLLGIARSFRSSRLLPVIQYEQPSCTVFGQVDLAQGG
HSNLGVSRDRQGEFQIRIDGQNARSAAQLAEILPLQLINPDSFRLLEGAPKIRRQFLDWGVFHVEPRFMATWQRLQKALK
QRNSWLRHGTLDAASQAAWDRELCSASDEIDEFRRAYIKALKPVFEQTLSELVELEGLTLSYYRGWDKEKELSTVLASSI
HRDQQMGHTQAGPQRADLRLRLGAHNAADILSRGQQKLVVCALRIAQGHLVSQVRRGQCIYLVDDLPSELDDNHRRALCR
LLEELRCQVFITCVDQEFLREGWQTETPVALFHVEQGRITQTHDHRE

Sequences:

>Translated_367_residues
MSLSRVSVTGVRNLHPVTLSPSPRINILYGANGSGKTSVLEAIHLLGIARSFRSSRLLPVIQYEQPSCTVFGQVDLAQGG
HSNLGVSRDRQGEFQIRIDGQNARSAAQLAEILPLQLINPDSFRLLEGAPKIRRQFLDWGVFHVEPRFMATWQRLQKALK
QRNSWLRHGTLDAASQAAWDRELCSASDEIDEFRRAYIKALKPVFEQTLSELVELEGLTLSYYRGWDKEKELSTVLASSI
HRDQQMGHTQAGPQRADLRLRLGAHNAADILSRGQQKLVVCALRIAQGHLVSQVRRGQCIYLVDDLPSELDDNHRRALCR
LLEELRCQVFITCVDQEFLREGWQTETPVALFHVEQGRITQTHDHRE
>Mature_366_residues
SLSRVSVTGVRNLHPVTLSPSPRINILYGANGSGKTSVLEAIHLLGIARSFRSSRLLPVIQYEQPSCTVFGQVDLAQGGH
SNLGVSRDRQGEFQIRIDGQNARSAAQLAEILPLQLINPDSFRLLEGAPKIRRQFLDWGVFHVEPRFMATWQRLQKALKQ
RNSWLRHGTLDAASQAAWDRELCSASDEIDEFRRAYIKALKPVFEQTLSELVELEGLTLSYYRGWDKEKELSTVLASSIH
RDQQMGHTQAGPQRADLRLRLGAHNAADILSRGQQKLVVCALRIAQGHLVSQVRRGQCIYLVDDLPSELDDNHRRALCRL
LEELRCQVFITCVDQEFLREGWQTETPVALFHVEQGRITQTHDHRE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=360, Percent_Identity=41.1111111111111, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 41580; Mature: 41449

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSRVSVTGVRNLHPVTLSPSPRINILYGANGSGKTSVLEAIHLLGIARSFRSSRLLPV
CCCCEEEECCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
IQYEQPSCTVFGQVDLAQGGHSNLGVSRDRQGEFQIRIDGQNARSAAQLAEILPLQLINP
EEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEECC
DSFRLLEGAPKIRRQFLDWGVFHVEPRFMATWQRLQKALKQRNSWLRHGTLDAASQAAWD
CCHHHHCCCHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RELCSASDEIDEFRRAYIKALKPVFEQTLSELVELEGLTLSYYRGWDKEKELSTVLASSI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHH
HRDQQMGHTQAGPQRADLRLRLGAHNAADILSRGQQKLVVCALRIAQGHLVSQVRRGQCI
HHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEE
YLVDDLPSELDDNHRRALCRLLEELRCQVFITCVDQEFLREGWQTETPVALFHVEQGRIT
EEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCCCEEEEEECCCCEE
QTHDHRE
CCCCCCC
>Mature Secondary Structure 
SLSRVSVTGVRNLHPVTLSPSPRINILYGANGSGKTSVLEAIHLLGIARSFRSSRLLPV
CCCEEEECCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
IQYEQPSCTVFGQVDLAQGGHSNLGVSRDRQGEFQIRIDGQNARSAAQLAEILPLQLINP
EEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEECC
DSFRLLEGAPKIRRQFLDWGVFHVEPRFMATWQRLQKALKQRNSWLRHGTLDAASQAAWD
CCHHHHCCCHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RELCSASDEIDEFRRAYIKALKPVFEQTLSELVELEGLTLSYYRGWDKEKELSTVLASSI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHH
HRDQQMGHTQAGPQRADLRLRLGAHNAADILSRGQQKLVVCALRIAQGHLVSQVRRGQCI
HHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEE
YLVDDLPSELDDNHRRALCRLLEELRCQVFITCVDQEFLREGWQTETPVALFHVEQGRIT
EEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCCCEEEEEECCCCEE
QTHDHRE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA