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The map label for this gene is shiA [H]

Identifier: 62391476

GI number: 62391476

Start: 2778419

End: 2779276

Strand: Reverse

Name: shiA [H]

Synonym: cg2920

Alternate gene names: 62391476

Gene position: 2779276-2778419 (Counterclockwise)

Preceding gene: 62391477

Following gene: 62391475

Centisome position: 84.66

GC content: 54.66

Gene sequence:

>858_bases
ATGGAACATTCTCCTGAAGGCAAGCGTGGATTCTTCACCTCATCGGTGATGGCGGGTTGCTCAGTTGGAAACGTCCTGGC
TGGCTTGGTATTTATCCCGTTCTTGATGCTGCCGGAAGAACACCTCATGTCATGGGGCTGGCGCGTACCTTTCCTGCTTT
CCGCACTGGTTTTAGTTGTCGCATACTTCGTGCGCACCCGACTGGAGGAAGCATCAACTGAGAAGGCCGAAGAGGACGCA
GGCGCTCCGGCTTTGGCTGTGCTGCGCACCCAGGGCATTGATGTCGCACGAGTTTTCCTGATCACCTTCTTCGCCGTTGT
TCAGACCACTTTCAACGTTTACGCACTGGCATACGCCGCCAACGAAATCGGCATCGATCGTTCCTTCATGGTGATGGTGA
ACACCATCGCGCTGGGGCTTTCCATCGGAACGATTCCTTTGGCCGCGTGGGTCTCTGACCGCATTGGCCGCAAGCCAGTC
TTGCTGTTCGGGGCCATCACCTGTGCAATCACCACCTACTTCTACTTCCAGGCAATCTCTGAAGCTGACCTTGTGCTGAT
CTTCGCACTGTGCTTGGTCAACCAAGGTTTGTTCTACTCCTGCTGGAACGGCGTGTGGACCATTTTCTTCCCAGAAATGT
TCGCATCTTCCGTGCGCTACACCGGCATGGCTATGGGCAACCAGCTCGGTCTGATCATCGTTGGTTTCGCACCAACCATC
GCCACCGCCCTGTACGCATGGAACGGTTGGGAAGCTGTTGCGGGATTCATCATCGGCGCAATCGCACTGTCTGCCGCAGT
TATTTTGACCACCAAGGAAACCGCCTTCACCAAGCTTGAAGATCTAGGGAAGAAATAA

Upstream 100 bases:

>100_bases
CGGACAGATCGGCATTTGGGCGACCGTGCTGTTGATGATCGCCCGCATCGCATAGGGATTCTCTGCAGTCGCAGAAGCTG
CAGGTGCATCCACACTGACC

Downstream 100 bases:

>100_bases
TGTCTGACAAGATCTGGAAAGTCGGCATCATCGGTTGCGGTGCAATCAGCCGAAACCATATCGAAGCAGTTCAGGCAATC
CCCGGCGCAGAAGTCAGCGC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEHSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEASTEKAEEDA
GAPALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYAANEIGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPV
LLFGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTI
ATALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK

Sequences:

>Translated_285_residues
MEHSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEASTEKAEEDA
GAPALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYAANEIGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPV
LLFGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTI
ATALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK
>Mature_285_residues
MEHSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEASTEKAEEDA
GAPALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYAANEIGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPV
LLFGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTI
ATALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=287, Percent_Identity=29.9651567944251, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790550, Length=298, Percent_Identity=25.8389261744966, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789941, Length=293, Percent_Identity=26.6211604095563, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788942, Length=234, Percent_Identity=26.0683760683761, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 30979; Mature: 30979

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVV
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
AYFVRTRLEEASTEKAEEDAGAPALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYAA
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPVLLFGAITCAITTYFYFQAIS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
EADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTI
CCHHHHHHHHHHHCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
ATALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK
HHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHCCC
>Mature Secondary Structure
MEHSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVV
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
AYFVRTRLEEASTEKAEEDAGAPALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYAA
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPVLLFGAITCAITTYFYFQAIS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
EADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTI
CCHHHHHHHHHHHCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
ATALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK
HHHHHHCCCHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]