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The map label for this gene is putA [C]

Identifier: 59714013

GI number: 59714013

Start: 933040

End: 933747

Strand: Reverse

Name: putA [C]

Synonym: VF_A0830

Alternate gene names: 59714013

Gene position: 933747-933040 (Counterclockwise)

Preceding gene: 172087817

Following gene: 59714012

Centisome position: 70.19

GC content: 40.82

Gene sequence:

>708_bases
ATGACTAAAATGACACAAATCATTGATTCAGTGCTTTCTCAAGGATTGACTGAGTGGCAATCTGAGTCAGCATCATTTCG
AATTGAAAAATTACTTACTTGGGCCAATCAGTTAGCTGAGAATCCACATTTTGGTGTACTTGCGGCTAAAATGGTTCGAT
ATCAATGTCAGCGAGCGCAACAACTTATCACTGACGTTGAGTTAATGCCCGGACCTACAGGTGAAAGTAATGAACTTTAT
ACCGCTCCAAGAGGTGTTTTTTACATCACATCGGATAAAAAAGCATCGGTTGTTGCATTGATTGGTCAAATAACTGCAGC
ATTAGTAGCCGGAAACGCTATTATTGTCGCCCTTTCGGATGAAGACTCTGAAGTGACTTCTGATATTGTTAGTAGCTTGC
ATGAGGTGAATGTTCCTGAATCTACCGTTCAGCAAGTGGATAGAGAAGCAGTAGACATAAATGATGAGCGGTTAGCTGGT
GTCTGTTTTGTTGGAGATATTCAAAAAGCAATTGAATTGAATCAACAATTAGCACAAAGGGATGGATTATTAGCGCAATT
GATCGCAGAAACGGATCAAGAAGGTTTAACGACAATTACTGATCCTCATTTTGTTCTACGCTTTATTACTGAGCGCACTC
GAACAATTAATGTTACAGCGGTAGGTGGTAACGCTACGCTGCTGGAACTTGGAGGTGGTAACCACTGA

Upstream 100 bases:

>100_bases
AAGGGTTATCGGGGACTGGACCTAAAGCGGGTGGGCCTCGTTATTTAATTCGTTATACAAAAGCAAATACAACGACTTCA
AAATGAGAAAGGAGATCTCC

Downstream 100 bases:

>100_bases
TATTCAATAGGATGAATATTACACCGTCATTTTTGCGCCATAACTATAGAGCAAAGCTCAACATAACAAGAGTGACGTTA
ATTACTGCCAATAACACAAA

Product: delta-1-pyrroline-5-carboxylate dehydrogenase

Products: L-glutamate; NADH; H+

Alternate protein names: Delta 1-Pyrroline-5-Carboxylate Dehydrogenase; Proline Dehydrogenase; Proline Dehydrogenase PutA; Aldehyde Dehydrogenase Family Protein; Proline Dehydrogenase Family; Bifunctional Protein Puta; 1-Pyrroline-5-Carboxylate Dehydrogenase; Bifunctional PutA Protein; Delta 1-Pyrroline-5-Carboxylate Dehydrogenase-Like Protein

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTKMTQIIDSVLSQGLTEWQSESASFRIEKLLTWANQLAENPHFGVLAAKMVRYQCQRAQQLITDVELMPGPTGESNELY
TAPRGVFYITSDKKASVVALIGQITAALVAGNAIIVALSDEDSEVTSDIVSSLHEVNVPESTVQQVDREAVDINDERLAG
VCFVGDIQKAIELNQQLAQRDGLLAQLIAETDQEGLTTITDPHFVLRFITERTRTINVTAVGGNATLLELGGGNH

Sequences:

>Translated_235_residues
MTKMTQIIDSVLSQGLTEWQSESASFRIEKLLTWANQLAENPHFGVLAAKMVRYQCQRAQQLITDVELMPGPTGESNELY
TAPRGVFYITSDKKASVVALIGQITAALVAGNAIIVALSDEDSEVTSDIVSSLHEVNVPESTVQQVDREAVDINDERLAG
VCFVGDIQKAIELNQQLAQRDGLLAQLIAETDQEGLTTITDPHFVLRFITERTRTINVTAVGGNATLLELGGGNH
>Mature_234_residues
TKMTQIIDSVLSQGLTEWQSESASFRIEKLLTWANQLAENPHFGVLAAKMVRYQCQRAQQLITDVELMPGPTGESNELYT
APRGVFYITSDKKASVVALIGQITAALVAGNAIIVALSDEDSEVTSDIVSSLHEVNVPESTVQQVDREAVDINDERLAGV
CFVGDIQKAIELNQQLAQRDGLLAQLIAETDQEGLTTITDPHFVLRFITERTRTINVTAVGGNATLLELGGGNH

Specific function: Oxidizes Proline To Glutamate For Use As A Carbon And Nitrogen Source And Also Function As A Transcriptional Repressor Of The Put Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.1.12

Molecular weight: Translated: 25535; Mature: 25404

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMTQIIDSVLSQGLTEWQSESASFRIEKLLTWANQLAENPHFGVLAAKMVRYQCQRAQ
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QLITDVELMPGPTGESNELYTAPRGVFYITSDKKASVVALIGQITAALVAGNAIIVALSD
HHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECC
EDSEVTSDIVSSLHEVNVPESTVQQVDREAVDINDERLAGVCFVGDIQKAIELNQQLAQR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHEEEEEHHHHHHHHHHHHHHHHH
DGLLAQLIAETDQEGLTTITDPHFVLRFITERTRTINVTAVGGNATLLELGGGNH
CCHHHHHHHHCCCCCCCEECCHHHHHHHHHHCCEEEEEEEECCCEEEEECCCCCC
>Mature Secondary Structure 
TKMTQIIDSVLSQGLTEWQSESASFRIEKLLTWANQLAENPHFGVLAAKMVRYQCQRAQ
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QLITDVELMPGPTGESNELYTAPRGVFYITSDKKASVVALIGQITAALVAGNAIIVALSD
HHHHHHHHCCCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECC
EDSEVTSDIVSSLHEVNVPESTVQQVDREAVDINDERLAGVCFVGDIQKAIELNQQLAQR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHEEEEEHHHHHHHHHHHHHHHHH
DGLLAQLIAETDQEGLTTITDPHFVLRFITERTRTINVTAVGGNATLLELGGGNH
CCHHHHHHHHCCCCCCCEECCHHHHHHHHHHCCEEEEEEEECCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 1-pyrroline-5-carboxylate; NAD+; H2O

Specific reaction: 1-pyrroline-5-carboxylate + NAD+ + H2O = L-glutamate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA