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The map label for this gene is bioD

Identifier: 59713928

GI number: 59713928

Start: 841432

End: 842112

Strand: Reverse

Name: bioD

Synonym: VF_A0745

Alternate gene names: 59713928

Gene position: 842112-841432 (Counterclockwise)

Preceding gene: 59713929

Following gene: 59713926

Centisome position: 63.3

GC content: 39.06

Gene sequence:

>681_bases
ATGATTGATGCATTTTTTATTGCAGGAACAGATACAGATGTGGGTAAAACCGTTGCTTCAAAAGCGATTTTAGATGCGTT
AAACATGAAAGGTTTACGAACTGCTGCTTATAAACCAGTAGCAGCAGGCTCTGAAGACAAAGGCGAAGGCGTTCAAAACT
CTGATGCGATCCATTTACGTGCTGTAGCCAATGTTGAATTACGCTACGAAGAAGTAAACCCGTATGCATTATTAATGCCT
GCATCACCACACATTGCAGCAGAAGCAGAAAATGTGGTTATTGATTACTCCGTATTATCTGAAGGTTTAGCTTCATTAAA
AGAGAAATCAGATGTTGTTTTAGTTGAAGGTGCCGGTGGATGGCGTGTTCCTGTATCTAAAGATGATTGTTTATCAACAT
GGGTACAGCAAGAAAAATTGCCAGTGGTATTGGTTGTTGGGATTAAACTCGGTTGTTTAAGTCATGCAATGCTAACTGCT
GAAGCGATTCAACATGATGGTTTAGAGATTATTGGTTGGGTAGCTAACCGAGTAAACCCAGGTACAGAAAACTACGCTGA
AATCATTAAGATGCTAGAAGATAAAATGCCTGCGCCAAAATTAGGTGAAATTCCATATATGCCAAGTGTGAAACGCAAAA
ATATGGGTAAATACATTCATCTTGAAGCCCTAGATAACTAA

Upstream 100 bases:

>100_bases
AAAGAAAAGCTATTAGCAGTGGAAGAAGCGTATCAACTATTTAGAAGTGAATTAAACCACTTACCTGCTACATATCAGGT
AGGCTACGGAGTTATAAGTA

Downstream 100 bases:

>100_bases
TTATTTAGTTTACTGTTTAAGTAAAAAGAATAAAGCCAATATCATTTGATGATATTGGCTTTATATTTAGGGCTCACTTG
GGCTAGTTGAGTAGTTATAA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIDAFFIAGTDTDVGKTVASKAILDALNMKGLRTAAYKPVAAGSEDKGEGVQNSDAIHLRAVANVELRYEEVNPYALLMP
ASPHIAAEAENVVIDYSVLSEGLASLKEKSDVVLVEGAGGWRVPVSKDDCLSTWVQQEKLPVVLVVGIKLGCLSHAMLTA
EAIQHDGLEIIGWVANRVNPGTENYAEIIKMLEDKMPAPKLGEIPYMPSVKRKNMGKYIHLEALDN

Sequences:

>Translated_226_residues
MIDAFFIAGTDTDVGKTVASKAILDALNMKGLRTAAYKPVAAGSEDKGEGVQNSDAIHLRAVANVELRYEEVNPYALLMP
ASPHIAAEAENVVIDYSVLSEGLASLKEKSDVVLVEGAGGWRVPVSKDDCLSTWVQQEKLPVVLVVGIKLGCLSHAMLTA
EAIQHDGLEIIGWVANRVNPGTENYAEIIKMLEDKMPAPKLGEIPYMPSVKRKNMGKYIHLEALDN
>Mature_226_residues
MIDAFFIAGTDTDVGKTVASKAILDALNMKGLRTAAYKPVAAGSEDKGEGVQNSDAIHLRAVANVELRYEEVNPYALLMP
ASPHIAAEAENVVIDYSVLSEGLASLKEKSDVVLVEGAGGWRVPVSKDDCLSTWVQQEKLPVVLVVGIKLGCLSHAMLTA
EAIQHDGLEIIGWVANRVNPGTENYAEIIKMLEDKMPAPKLGEIPYMPSVKRKNMGKYIHLEALDN

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI87081944, Length=224, Percent_Identity=52.6785714285714, Blast_Score=239, Evalue=9e-65,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=51.1111111111111, Blast_Score=231, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_VIBF1 (Q5DZI1)

Other databases:

- EMBL:   CP000021
- RefSeq:   YP_206703.1
- HSSP:   P13000
- ProteinModelPortal:   Q5DZI1
- SMR:   Q5DZI1
- STRING:   Q5DZI1
- GeneID:   3280456
- GenomeReviews:   CP000021_GR
- KEGG:   vfi:VF_A0745
- NMPDR:   fig|312309.3.peg.3601
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   IDTIVAC
- PhylomeDB:   Q5DZI1
- ProtClustDB:   PRK00090
- BioCyc:   VFIS312309:VFA0745-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 24409; Mature: 24409

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDAFFIAGTDTDVGKTVASKAILDALNMKGLRTAAYKPVAAGSEDKGEGVQNSDAIHLR
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEE
AVANVELRYEEVNPYALLMPASPHIAAEAENVVIDYSVLSEGLASLKEKSDVVLVEGAGG
EEEEEEEEEEECCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCC
WRVPVSKDDCLSTWVQQEKLPVVLVVGIKLGCLSHAMLTAEAIQHDGLEIIGWVANRVNP
EECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHEEEEHHHCCCC
GTENYAEIIKMLEDKMPAPKLGEIPYMPSVKRKNMGKYIHLEALDN
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MIDAFFIAGTDTDVGKTVASKAILDALNMKGLRTAAYKPVAAGSEDKGEGVQNSDAIHLR
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEE
AVANVELRYEEVNPYALLMPASPHIAAEAENVVIDYSVLSEGLASLKEKSDVVLVEGAGG
EEEEEEEEEEECCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCC
WRVPVSKDDCLSTWVQQEKLPVVLVVGIKLGCLSHAMLTAEAIQHDGLEIIGWVANRVNP
EECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHEEEEHHHCCCC
GTENYAEIIKMLEDKMPAPKLGEIPYMPSVKRKNMGKYIHLEALDN
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA