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The map label for this gene is cusS [H]

Identifier: 59713618

GI number: 59713618

Start: 498318

End: 499667

Strand: Reverse

Name: cusS [H]

Synonym: VF_A0435

Alternate gene names: 59713618

Gene position: 499667-498318 (Counterclockwise)

Preceding gene: 59713619

Following gene: 59713611

Centisome position: 37.56

GC content: 37.93

Gene sequence:

>1350_bases
ATGTTCTTAGAACAACGTGATGAGCTTTACCGTTCCTCCATTTTAAAATTATTAATATGGTTTGCTATCGGGTTATTTTT
AGTTATGGGGTTGATTATTTACCAACTCTATTCAACTTCTGTTCAACTGTATAACCACAACCTTGATGAATGGCTTAGAG
CTGAAACGGCGCAACTACAAGCGATTGTCGATACAGAGGGTGTCGATGCCATTCAACAAAATATTAATAATACTCAAGGT
GATAGCCGATACATTTATCAATTCGTTCATCATGCATCGCCAACACCAACGAATACTGCAGCAACGGCTTATCCTGTTAT
TGCACAAATAAAACAATCTTCTACAAATACTGACCTTCCTGATATGCGGGCAACTAAAATAAATCTACCTGATGGTTCGG
AACTTGTCGTTGGTATTGACCAAAATCGCTATCAGAGTTTTAAGAAGCAATTAGATTCATCGGTAATTTGGGGAACGTTC
TTTCCTTTTATTGCCCTGTGTTTAATCGCAATTCTCATTGCTGTCTACATTCTAAAGCGCCTTAACCTCGTTAATGCAAC
AATGAATCGAGTGATGCTTGGTGAGCGTAATGTACGGTTAAAAGTCGGTCCTGATATGAACGAATTCGATTTATTGGCTC
TTCATTTAAATTCGATGCTTGAACAAATGGAGCAAAGTGAGTCTAAGTTAAAATCATTAACTGTCGATATTGCTCATGAT
CTCAGAACCCCTATGGGGCGAATTAAATTAAGAATAGAAGATCTGCTCTATGACGAGTCTCTTTCTCAACAACACACCGA
ACAATTAGATAATATACAGTCTGACTTTTCTTTATTACTTGAAACCTTCAATGGCATGATGGAGCTATATCATCTTGAAA
ATGGAGGCACTCCTATCAATAAAGAAATGTGCGATTTAAGTAAAATTGTACGTGATGCCATTGAATTTGCAGAGCCTACA
GTAGCGGATAAAAAACAAACTCTTCATTTCACAGAAGAAATGCCTTGTTTACTTTACGCTAACCCTAGCCTTATTTTCCG
TGCCGTATTCAACATTATTGATAACGCCATTAAGTACACTCATGAAGGTGGCGTTATCGAAGTTATTGTCGATTGTTTTG
GTGTGGTGGTTGCTGATAATGGTAGAGGCATAAAACCGGAAGATAGAGAGCGCGCCTTAGGTCAATTAGTTAGATTAGAC
CCGAGTCGCACTCAACATGGTTTTGGTTTAGGACTCGCATTAGTTAATACGGTCATGAAACTACACGATGGACAGATCAG
TTTAAGTGATAATTATCCAGGATTAAGAGCTCGATTGCTGTTCCAAGAAAAATTAGAACCTGAGTTATAA

Upstream 100 bases:

>100_bases
CCAAAAACCAATGTTATTGATGTTCATATTGCTAAGTTACGCAAAAAATTAGATAACTCTGGAGCTGTGGATCTCATCGA
AACCGTACGTGGAGCTGGTT

Downstream 100 bases:

>100_bases
CAACCTCTATCTCAATATATTCCACGCTTCACACATTATTCTAAAAGTATTAGCATCACCGGTTTCTCTGTCTGGGTGGT
GCTTCATTCCTAGTTTTCGC

Product: two component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MFLEQRDELYRSSILKLLIWFAIGLFLVMGLIIYQLYSTSVQLYNHNLDEWLRAETAQLQAIVDTEGVDAIQQNINNTQG
DSRYIYQFVHHASPTPTNTAATAYPVIAQIKQSSTNTDLPDMRATKINLPDGSELVVGIDQNRYQSFKKQLDSSVIWGTF
FPFIALCLIAILIAVYILKRLNLVNATMNRVMLGERNVRLKVGPDMNEFDLLALHLNSMLEQMEQSESKLKSLTVDIAHD
LRTPMGRIKLRIEDLLYDESLSQQHTEQLDNIQSDFSLLLETFNGMMELYHLENGGTPINKEMCDLSKIVRDAIEFAEPT
VADKKQTLHFTEEMPCLLYANPSLIFRAVFNIIDNAIKYTHEGGVIEVIVDCFGVVVADNGRGIKPEDRERALGQLVRLD
PSRTQHGFGLGLALVNTVMKLHDGQISLSDNYPGLRARLLFQEKLEPEL

Sequences:

>Translated_449_residues
MFLEQRDELYRSSILKLLIWFAIGLFLVMGLIIYQLYSTSVQLYNHNLDEWLRAETAQLQAIVDTEGVDAIQQNINNTQG
DSRYIYQFVHHASPTPTNTAATAYPVIAQIKQSSTNTDLPDMRATKINLPDGSELVVGIDQNRYQSFKKQLDSSVIWGTF
FPFIALCLIAILIAVYILKRLNLVNATMNRVMLGERNVRLKVGPDMNEFDLLALHLNSMLEQMEQSESKLKSLTVDIAHD
LRTPMGRIKLRIEDLLYDESLSQQHTEQLDNIQSDFSLLLETFNGMMELYHLENGGTPINKEMCDLSKIVRDAIEFAEPT
VADKKQTLHFTEEMPCLLYANPSLIFRAVFNIIDNAIKYTHEGGVIEVIVDCFGVVVADNGRGIKPEDRERALGQLVRLD
PSRTQHGFGLGLALVNTVMKLHDGQISLSDNYPGLRARLLFQEKLEPEL
>Mature_449_residues
MFLEQRDELYRSSILKLLIWFAIGLFLVMGLIIYQLYSTSVQLYNHNLDEWLRAETAQLQAIVDTEGVDAIQQNINNTQG
DSRYIYQFVHHASPTPTNTAATAYPVIAQIKQSSTNTDLPDMRATKINLPDGSELVVGIDQNRYQSFKKQLDSSVIWGTF
FPFIALCLIAILIAVYILKRLNLVNATMNRVMLGERNVRLKVGPDMNEFDLLALHLNSMLEQMEQSESKLKSLTVDIAHD
LRTPMGRIKLRIEDLLYDESLSQQHTEQLDNIQSDFSLLLETFNGMMELYHLENGGTPINKEMCDLSKIVRDAIEFAEPT
VADKKQTLHFTEEMPCLLYANPSLIFRAVFNIIDNAIKYTHEGGVIEVIVDCFGVVVADNGRGIKPEDRERALGQLVRLD
PSRTQHGFGLGLALVNTVMKLHDGQISLSDNYPGLRARLLFQEKLEPEL

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=287, Percent_Identity=26.1324041811847, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790346, Length=244, Percent_Identity=29.0983606557377, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789808, Length=296, Percent_Identity=27.3648648648649, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=25.296442687747, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=284, Percent_Identity=26.056338028169, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790861, Length=314, Percent_Identity=25.4777070063694, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=25.8620689655172, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789403, Length=314, Percent_Identity=22.9299363057325, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=20.353982300885, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=24.7967479674797, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1788713, Length=210, Percent_Identity=24.7619047619048, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50907; Mature: 50907

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEQRDELYRSSILKLLIWFAIGLFLVMGLIIYQLYSTSVQLYNHNLDEWLRAETAQLQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIVDTEGVDAIQQNINNTQGDSRYIYQFVHHASPTPTNTAATAYPVIAQIKQSSTNTDLP
HHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DMRATKINLPDGSELVVGIDQNRYQSFKKQLDSSVIWGTFFPFIALCLIAILIAVYILKR
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLVNATMNRVMLGERNVRLKVGPDMNEFDLLALHLNSMLEQMEQSESKLKSLTVDIAHD
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRTPMGRIKLRIEDLLYDESLSQQHTEQLDNIQSDFSLLLETFNGMMELYHLENGGTPIN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
KEMCDLSKIVRDAIEFAEPTVADKKQTLHFTEEMPCLLYANPSLIFRAVFNIIDNAIKYT
HHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHCCCCEEEECCHHHHHHHHHHHHHHHHHHC
HEGGVIEVIVDCFGVVVADNGRGIKPEDRERALGQLVRLDPSRTQHGFGLGLALVNTVMK
CCCCHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LHDGQISLSDNYPGLRARLLFQEKLEPEL
HCCCEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFLEQRDELYRSSILKLLIWFAIGLFLVMGLIIYQLYSTSVQLYNHNLDEWLRAETAQLQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIVDTEGVDAIQQNINNTQGDSRYIYQFVHHASPTPTNTAATAYPVIAQIKQSSTNTDLP
HHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DMRATKINLPDGSELVVGIDQNRYQSFKKQLDSSVIWGTFFPFIALCLIAILIAVYILKR
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLVNATMNRVMLGERNVRLKVGPDMNEFDLLALHLNSMLEQMEQSESKLKSLTVDIAHD
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRTPMGRIKLRIEDLLYDESLSQQHTEQLDNIQSDFSLLLETFNGMMELYHLENGGTPIN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
KEMCDLSKIVRDAIEFAEPTVADKKQTLHFTEEMPCLLYANPSLIFRAVFNIIDNAIKYT
HHHHHHHHHHHHHHHHCCCCCCCCHHHEEHHCCCCEEEECCHHHHHHHHHHHHHHHHHHC
HEGGVIEVIVDCFGVVVADNGRGIKPEDRERALGQLVRLDPSRTQHGFGLGLALVNTVMK
CCCCHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LHDGQISLSDNYPGLRARLLFQEKLEPEL
HCCCEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]