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The map label for this gene is galR [H]

Identifier: 59713544

GI number: 59713544

Start: 406842

End: 407840

Strand: Reverse

Name: galR [H]

Synonym: VF_A0361

Alternate gene names: 59713544

Gene position: 407840-406842 (Counterclockwise)

Preceding gene: 59713554

Following gene: 59713541

Centisome position: 30.66

GC content: 37.54

Gene sequence:

>999_bases
ATGGCAACCATTAAAGATGTAGCAAAAGAAGCTGGTGTATCTGTTGCCACCGTTTCTCGTGTAGTAAATAAATCACCTAA
AGCCAGTAAAGCAGCCATAAAATCTGTTAATGCTGCAATGACAAAACTCGGCTATCGACCTAATGCTAATGCTCGTGCTT
TAGTCAGTAAAAGTACCAATACTATTGGGGTATTAGTTGCAGATGTTTCTGACCCATTTTTTGGATCTTTAATCAAAGCC
GTTGATGGTGTAGCTAGAGCCAACAACAAACATTTATTAATTGGTAATGGTTATCACAATGCTGAAGTTGAGAAAGAGGC
CATAGAGCTATTAATCCATAACCGTTGTGAATCTCTGATTATTCACTCTAAAGGATTAAGTGATGAGGAATTAATTCAAT
TTTCACAAGAAGTACCTGGATTGGTCATTATTAATCGTCATATCCCCCAAATAGCTCATAAATGCATCTATCTAGATAAC
CAACTTGGGTCAAGACTTGCCACAGAACACCTTATTGAAAGTGGTCACCAACATTTAGCCTACATCAACTCTAATCAACA
AATTGATGATTGTATTGATCGAAAGCACGGTTTTGCTCAAGCCTTAGAAAAACATGGATTAATCATTAATAGTTCTTTAT
TAAGTGAAGGCGAACCCACTGACGAAGGGGGGGAAAACGCGATGAATGAATTAATTAAAAATAAACAATCATTTACGGGT
GTTGTTTGTTATAACGATTACATGGCAGCGGGAGCTATTGCAGCCCTTGAAAAAGCAGGTATTTCAGTACCAGAGCAGGT
TTCCATTATTGGTTTCGATAACGGTCTAATTGCCAAATATCTACACCCTAAACTAACAACCATTGACTACCCTATTAGTG
AGATGGCCTCACAAGCAGCCCTGTTATCGCTATTGCTAGCAAAAGATGAAAAACCAGAAACAGAGAAAAAAGTATATACG
CCAACACTCGTTAAACGTAATTCAGTACATACTCTATAA

Upstream 100 bases:

>100_bases
AACGTTTACAAAATCAATCCTATTGCATTAAGATTGAAAACGAAATAAAAGTTTACTAAAACTTTAACTCGATCAAATTA
ATAATAACTAAGGCTCCATT

Downstream 100 bases:

>100_bases
ATAAAATGAGCGTATATATGAATCATTATTTATGTATACGCTTCCAATTTTTCTAGTATAAGCTCTTTTCTAAATGGTTT
TGCTACATAATCATCCATGC

Product: DNA-binding transcriptional repressor

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MATIKDVAKEAGVSVATVSRVVNKSPKASKAAIKSVNAAMTKLGYRPNANARALVSKSTNTIGVLVADVSDPFFGSLIKA
VDGVARANNKHLLIGNGYHNAEVEKEAIELLIHNRCESLIIHSKGLSDEELIQFSQEVPGLVIINRHIPQIAHKCIYLDN
QLGSRLATEHLIESGHQHLAYINSNQQIDDCIDRKHGFAQALEKHGLIINSSLLSEGEPTDEGGENAMNELIKNKQSFTG
VVCYNDYMAAGAIAALEKAGISVPEQVSIIGFDNGLIAKYLHPKLTTIDYPISEMASQAALLSLLLAKDEKPETEKKVYT
PTLVKRNSVHTL

Sequences:

>Translated_332_residues
MATIKDVAKEAGVSVATVSRVVNKSPKASKAAIKSVNAAMTKLGYRPNANARALVSKSTNTIGVLVADVSDPFFGSLIKA
VDGVARANNKHLLIGNGYHNAEVEKEAIELLIHNRCESLIIHSKGLSDEELIQFSQEVPGLVIINRHIPQIAHKCIYLDN
QLGSRLATEHLIESGHQHLAYINSNQQIDDCIDRKHGFAQALEKHGLIINSSLLSEGEPTDEGGENAMNELIKNKQSFTG
VVCYNDYMAAGAIAALEKAGISVPEQVSIIGFDNGLIAKYLHPKLTTIDYPISEMASQAALLSLLLAKDEKPETEKKVYT
PTLVKRNSVHTL
>Mature_331_residues
ATIKDVAKEAGVSVATVSRVVNKSPKASKAAIKSVNAAMTKLGYRPNANARALVSKSTNTIGVLVADVSDPFFGSLIKAV
DGVARANNKHLLIGNGYHNAEVEKEAIELLIHNRCESLIIHSKGLSDEELIQFSQEVPGLVIINRHIPQIAHKCIYLDNQ
LGSRLATEHLIESGHQHLAYINSNQQIDDCIDRKHGFAQALEKHGLIINSSLLSEGEPTDEGGENAMNELIKNKQSFTGV
VCYNDYMAAGAIAALEKAGISVPEQVSIIGFDNGLIAKYLHPKLTTIDYPISEMASQAALLSLLLAKDEKPETEKKVYTP
TLVKRNSVHTL

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=51.2048192771084, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=50.1510574018127, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1789068, Length=335, Percent_Identity=34.0298507462687, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=32.8313253012048, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=31.6715542521994, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=31.2312312312312, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787906, Length=316, Percent_Identity=29.746835443038, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI48994940, Length=302, Percent_Identity=25.8278145695364, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=29.6296296296296, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786540, Length=291, Percent_Identity=28.5223367697594, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790715, Length=313, Percent_Identity=23.6421725239617, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790689, Length=300, Percent_Identity=22.6666666666667, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35948; Mature: 35816

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKEAGVSVATVSRVVNKSPKASKAAIKSVNAAMTKLGYRPNANARALVSKSTN
CCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
TIGVLVADVSDPFFGSLIKAVDGVARANNKHLLIGNGYHNAEVEKEAIELLIHNRCESLI
EEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEE
IHSKGLSDEELIQFSQEVPGLVIINRHIPQIAHKCIYLDNQLGSRLATEHLIESGHQHLA
EECCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECHHHHHHHHHHHHHHCCCCEEE
YINSNQQIDDCIDRKHGFAQALEKHGLIINSSLLSEGEPTDEGGENAMNELIKNKQSFTG
EECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCHHHHHHHHHHCHHHCEE
VVCYNDYMAAGAIAALEKAGISVPEQVSIIGFDNGLIAKYLHPKLTTIDYPISEMASQAA
EEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCEEECCCHHHHHHHHH
LLSLLLAKDEKPETEKKVYTPTLVKRNSVHTL
HHHHHHHCCCCCCCHHHCCCCHHEECCCCCCC
>Mature Secondary Structure 
ATIKDVAKEAGVSVATVSRVVNKSPKASKAAIKSVNAAMTKLGYRPNANARALVSKSTN
CCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
TIGVLVADVSDPFFGSLIKAVDGVARANNKHLLIGNGYHNAEVEKEAIELLIHNRCESLI
EEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEE
IHSKGLSDEELIQFSQEVPGLVIINRHIPQIAHKCIYLDNQLGSRLATEHLIESGHQHLA
EECCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHEECHHHHHHHHHHHHHHCCCCEEE
YINSNQQIDDCIDRKHGFAQALEKHGLIINSSLLSEGEPTDEGGENAMNELIKNKQSFTG
EECCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCHHHHHHHHHHCHHHCEE
VVCYNDYMAAGAIAALEKAGISVPEQVSIIGFDNGLIAKYLHPKLTTIDYPISEMASQAA
EEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCEEECCCHHHHHHHHH
LLSLLLAKDEKPETEKKVYTPTLVKRNSVHTL
HHHHHHHCCCCCCCHHHCCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]