Click here to switch to the map view.

The map label for this gene is pspF [H]

Identifier: 59713493

GI number: 59713493

Start: 344937

End: 345956

Strand: Reverse

Name: pspF [H]

Synonym: VF_A0310

Alternate gene names: 59713493

Gene position: 345956-344937 (Counterclockwise)

Preceding gene: 59713506

Following gene: 59713492

Centisome position: 26.01

GC content: 37.84

Gene sequence:

>1020_bases
ATGAGAAAAGACAGTTTAGTTGGGGAGTCTGATGCATTCTTATCTGCGTTAGACCATGCTTCACAATTAGCGACAATTGA
TAGACCGATACTAATCCTTGGTGAAAGAGGAACAGGTAAAGAGTTAATTGCTCAGCGTTTGCATTTTTTATCTAGACGCT
GGAATGAGCCATTGATTAGCTTAAATTGTGCAGCTCTAAACTCCGGAACTCTCGATTCGGAACTTTTCGGCCACGATGCA
GGATCTTTTACGGGTGCAAAGCAACGTCATCAAGGTCGATTTGAACGTGCCGATGGCGGCTCTTTATTTTTAGATGAACT
CACTACTGCTCCTCTTACTGTACAAGAAAAGCTACTTAGAGTGATTGAGTATGGTGAATATGAACGAGTTGGTGGGTCAC
AAACATTAAAAGCCAATGTTCGTCTAATTTGTGCAACTAATCAAAACCCTCAAGAAATGGTTAATGAGAAAAAATTTCGA
GCAGATCTATTAGATAGACTCTCTTTTGATGTCATTCATCTACCACCACTGCGATACCGTGAAAACGATGTTTTATTACT
TGCAGAACATTTTGCAATCAAAATGTGTCAGGAAATGAACCTTACTTATTTCGCTGGTTTCTCGCACCAAGCTAAACATC
AAATTTTAGATTATGACTGGCCAGGTAATGTTCGCGAACTTCGTAATTGTATTGAACGAGCGGTATTTAAACATAACCAT
GAAGAAGCTCAGATAGAATCCATTCAAATAAACCCATTTATTGCACCTTGGGAAAATGCGCCAACAAAAAATTCTTCAAC
TATAATCAACAAGACGGTAACGCAAGATAGCAAACCATCTTTACCTTTGAATCTAAAGCAATTTCAATTAGATCAAGAAA
AAGAATTACTGCAACAAGCATTAGTTGAAACACAGTACCACCAACGAAAAGCTGCTGAAGAATTAGGCATTACTTACCAT
CAATTTCGTGGATTATTAAAAAAACATCAACTCTTGACCACAATCAAGGGATATTCTTAG

Upstream 100 bases:

>100_bases
TAAAAATCAATAACTTATTTAAAATTGACCACACAATAAAAATAAGTCATGATTAAAACCACTACTTTTTGGTCAAAATA
ACCATAAGAATGGAGATCTG

Downstream 100 bases:

>100_bases
ATATTTGAGTGCAGAACCAGTCACTTGGGTATAAAATGGCAGGTTCATTAATTTAAATCGATAAAACTACGGTCATTATG
AAGATTATTGCGCGCCTACT

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRKDSLVGESDAFLSALDHASQLATIDRPILILGERGTGKELIAQRLHFLSRRWNEPLISLNCAALNSGTLDSELFGHDA
GSFTGAKQRHQGRFERADGGSLFLDELTTAPLTVQEKLLRVIEYGEYERVGGSQTLKANVRLICATNQNPQEMVNEKKFR
ADLLDRLSFDVIHLPPLRYRENDVLLLAEHFAIKMCQEMNLTYFAGFSHQAKHQILDYDWPGNVRELRNCIERAVFKHNH
EEAQIESIQINPFIAPWENAPTKNSSTIINKTVTQDSKPSLPLNLKQFQLDQEKELLQQALVETQYHQRKAAEELGITYH
QFRGLLKKHQLLTTIKGYS

Sequences:

>Translated_339_residues
MRKDSLVGESDAFLSALDHASQLATIDRPILILGERGTGKELIAQRLHFLSRRWNEPLISLNCAALNSGTLDSELFGHDA
GSFTGAKQRHQGRFERADGGSLFLDELTTAPLTVQEKLLRVIEYGEYERVGGSQTLKANVRLICATNQNPQEMVNEKKFR
ADLLDRLSFDVIHLPPLRYRENDVLLLAEHFAIKMCQEMNLTYFAGFSHQAKHQILDYDWPGNVRELRNCIERAVFKHNH
EEAQIESIQINPFIAPWENAPTKNSSTIINKTVTQDSKPSLPLNLKQFQLDQEKELLQQALVETQYHQRKAAEELGITYH
QFRGLLKKHQLLTTIKGYS
>Mature_339_residues
MRKDSLVGESDAFLSALDHASQLATIDRPILILGERGTGKELIAQRLHFLSRRWNEPLISLNCAALNSGTLDSELFGHDA
GSFTGAKQRHQGRFERADGGSLFLDELTTAPLTVQEKLLRVIEYGEYERVGGSQTLKANVRLICATNQNPQEMVNEKKFR
ADLLDRLSFDVIHLPPLRYRENDVLLLAEHFAIKMCQEMNLTYFAGFSHQAKHQILDYDWPGNVRELRNCIERAVFKHNH
EEAQIESIQINPFIAPWENAPTKNSSTIINKTVTQDSKPSLPLNLKQFQLDQEKELLQQALVETQYHQRKAAEELGITYH
QFRGLLKKHQLLTTIKGYS

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=60.4863221884498, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=42.8571428571429, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=41.5335463258786, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=46.7532467532467, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1788550, Length=325, Percent_Identity=39.3846153846154, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1790299, Length=316, Percent_Identity=38.2911392405063, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=37.0253164556962, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1789233, Length=334, Percent_Identity=35.6287425149701, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1788905, Length=331, Percent_Identity=33.2326283987915, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786524, Length=216, Percent_Identity=43.9814814814815, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=31.9018404907975, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1789828, Length=215, Percent_Identity=32.093023255814, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081858, Length=220, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 38672; Mature: 38672

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDSLVGESDAFLSALDHASQLATIDRPILILGERGTGKELIAQRLHFLSRRWNEPLIS
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
LNCAALNSGTLDSELFGHDAGSFTGAKQRHQGRFERADGGSLFLDELTTAPLTVQEKLLR
EEEEECCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCEEHHHHCCCCCHHHHHHHH
VIEYGEYERVGGSQTLKANVRLICATNQNPQEMVNEKKFRADLLDRLSFDVIHLPPLRYR
HHHCCCHHHCCCCCEEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
ENDVLLLAEHFAIKMCQEMNLTYFAGFSHQAKHQILDYDWPGNVRELRNCIERAVFKHNH
CCCEEEEHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
EEAQIESIQINPFIAPWENAPTKNSSTIINKTVTQDSKPSLPLNLKQFQLDQEKELLQQA
CCCEEEEEEECCEEECCCCCCCCCCCEEEEHHHCCCCCCCCCCCHHHHCCCHHHHHHHHH
LVETQYHQRKAAEELGITYHQFRGLLKKHQLLTTIKGYS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKDSLVGESDAFLSALDHASQLATIDRPILILGERGTGKELIAQRLHFLSRRWNEPLIS
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
LNCAALNSGTLDSELFGHDAGSFTGAKQRHQGRFERADGGSLFLDELTTAPLTVQEKLLR
EEEEECCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCEEHHHHCCCCCHHHHHHHH
VIEYGEYERVGGSQTLKANVRLICATNQNPQEMVNEKKFRADLLDRLSFDVIHLPPLRYR
HHHCCCHHHCCCCCEEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
ENDVLLLAEHFAIKMCQEMNLTYFAGFSHQAKHQILDYDWPGNVRELRNCIERAVFKHNH
CCCEEEEHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
EEAQIESIQINPFIAPWENAPTKNSSTIINKTVTQDSKPSLPLNLKQFQLDQEKELLQQA
CCCEEEEEEECCEEECCCCCCCCCCCEEEEHHHCCCCCCCCCCCHHHHCCCHHHHHHHHH
LVETQYHQRKAAEELGITYHQFRGLLKKHQLLTTIKGYS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]