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The map label for this gene is sapA [H]

Identifier: 59713492

GI number: 59713492

Start: 343234

End: 344859

Strand: Reverse

Name: sapA [H]

Synonym: VF_A0309

Alternate gene names: 59713492

Gene position: 344859-343234 (Counterclockwise)

Preceding gene: 59713493

Following gene: 59713491

Centisome position: 25.92

GC content: 38.13

Gene sequence:

>1626_bases
ATGAAGATTATTGCGCGCCTACTCATAGCTGCTACTGGATTATTTAATCTAACAGCTTGTTATGATGCGGGGATCAAACA
AACGATTCATGACACAGGATTCATTTATTGTGAATCTGGAAATGTGGATTTTTTCAACCCACAGATTTCTGACGGCAACA
GCATTCTGGAGGCCATAAGCGCTCAAGTTTATGACCGTTTGCTTATCATAGCTCCTGTTACGCAAAAACCTTCAGCTAAC
CTCGCGACCTCTTGGCAGGTTGATGATACGGGTACAGTTTATACATTTGATTTAAAAGAAAATGTTCAATTTCATACAAC
CGAATGGTTTACTCCATCCCGAGCTTTAACAGCTAGTGATGTAGCATTCAGTTTTAATCGAATCTTAGATAAAAATAACG
CATTTCATAACGTTAATTCAGGAGACTATCCTTGGTTTAACAGCATAAACTTTTCTCAAATGGTTACATCTGTTGAAGCA
ATCACCCCTCATAAAGTGCGATTTACTTTATCAACACCAGATAACACTTTCTTATCAGTTCTCGCTGCAAGCTATGCGGT
TATTCATTCAGAGGAGTACGCACAACAATTAGCAGCAATAGACGAAAAAACATTAATTGATGTTAAGCCAGTAGGTACTG
GTCCATTTAAGTTAGCTCAATTCCAAAGAAGTGAATTAGTTCGCTTGAAGCGTCATGAACAATACTGGCAAGGTCCTGCA
AACATGTCTCAAGTTGTCTTTGATATCTCTAGCAGAGGGACAGGCTCTTTAGCAAAATTACTGCGCCAAGAATGCGATGT
TGTTGCCAATCCAATATCAAGCCAACTACCAATCATTATTGCTAATGATGCATTGACTTTATACTCACAAACGGCAATGA
ATGTAGCTTATATCGCAATTAATATGCGAGAACCGATATTACAAGATCAACGAGTTCGAAAGGCACTCAGTTTTGCTATC
GACCGTTCTAGCCTAATTAATTCGGTTTATTACAATTCAGGAATTGAAGCAAAATCCATTTTACCTCCCGCATCATGGGC
TTATGGTAATGACAGCTCACACATTCGCTATGATCTCAATTACGCCAAAGGCTTGTTACGAGAAGCCGGCGTTAAAGATG
GGTTAGAACTCACCATGTGGGTCCCGCTGGATAATAACTCATACAATCCAAGTCCGAAAAAATCTGCAGAGATTATTCAA
AGTGACTTTGCAAAATTAGGCATAGATTTAAAAATACTCACCTCTGATATTTTAAATCCGAATGAATTAAAAGATGCTGA
TGTCGATTTGGTGCTTACAGGCTGGAATGCAAACAGTTCAGATCCTGATTCATTACTTAGACCGTTACTTTCGTGTGATG
CTAAACAAGCGGGTTTTGGTGTTGCAAACTGGTGTAACGAGGAGTTTGATCTGTTATTAGATTTAGCTAAAGAAACAAAT
CAACCAAGATATCGTATTAATATTTATCGCCAAATCCAAAATGTATTAAGTGAAGAATTACCCGTTATCCCTCTGGCTCA
CGGTGTTAAATATCAAGCAAATCAATCTTCTTTAACAGGCTTCACATTAAGCCCTTTTAATACTTACTCTTTCCATAACG
TTGTTAGAGTTAAGGAGTTGAACTAA

Upstream 100 bases:

>100_bases
CCACAATCAAGGGATATTCTTAGATATTTGAGTGCAGAACCAGTCACTTGGGTATAAAATGGCAGGTTCATTAATTTAAA
TCGATAAAACTACGGTCATT

Downstream 100 bases:

>100_bases
TGTGGATTTATACCATTAGACGCTTTAATTTATTTGTTATTACCTTACTCATTTTAACTCTGATTGGTTTTAATATTTTA
CGCCTAGATGATACATCTGC

Product: peptide transport protein SapA

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKIIARLLIAATGLFNLTACYDAGIKQTIHDTGFIYCESGNVDFFNPQISDGNSILEAISAQVYDRLLIIAPVTQKPSAN
LATSWQVDDTGTVYTFDLKENVQFHTTEWFTPSRALTASDVAFSFNRILDKNNAFHNVNSGDYPWFNSINFSQMVTSVEA
ITPHKVRFTLSTPDNTFLSVLAASYAVIHSEEYAQQLAAIDEKTLIDVKPVGTGPFKLAQFQRSELVRLKRHEQYWQGPA
NMSQVVFDISSRGTGSLAKLLRQECDVVANPISSQLPIIIANDALTLYSQTAMNVAYIAINMREPILQDQRVRKALSFAI
DRSSLINSVYYNSGIEAKSILPPASWAYGNDSSHIRYDLNYAKGLLREAGVKDGLELTMWVPLDNNSYNPSPKKSAEIIQ
SDFAKLGIDLKILTSDILNPNELKDADVDLVLTGWNANSSDPDSLLRPLLSCDAKQAGFGVANWCNEEFDLLLDLAKETN
QPRYRINIYRQIQNVLSEELPVIPLAHGVKYQANQSSLTGFTLSPFNTYSFHNVVRVKELN

Sequences:

>Translated_541_residues
MKIIARLLIAATGLFNLTACYDAGIKQTIHDTGFIYCESGNVDFFNPQISDGNSILEAISAQVYDRLLIIAPVTQKPSAN
LATSWQVDDTGTVYTFDLKENVQFHTTEWFTPSRALTASDVAFSFNRILDKNNAFHNVNSGDYPWFNSINFSQMVTSVEA
ITPHKVRFTLSTPDNTFLSVLAASYAVIHSEEYAQQLAAIDEKTLIDVKPVGTGPFKLAQFQRSELVRLKRHEQYWQGPA
NMSQVVFDISSRGTGSLAKLLRQECDVVANPISSQLPIIIANDALTLYSQTAMNVAYIAINMREPILQDQRVRKALSFAI
DRSSLINSVYYNSGIEAKSILPPASWAYGNDSSHIRYDLNYAKGLLREAGVKDGLELTMWVPLDNNSYNPSPKKSAEIIQ
SDFAKLGIDLKILTSDILNPNELKDADVDLVLTGWNANSSDPDSLLRPLLSCDAKQAGFGVANWCNEEFDLLLDLAKETN
QPRYRINIYRQIQNVLSEELPVIPLAHGVKYQANQSSLTGFTLSPFNTYSFHNVVRVKELN
>Mature_541_residues
MKIIARLLIAATGLFNLTACYDAGIKQTIHDTGFIYCESGNVDFFNPQISDGNSILEAISAQVYDRLLIIAPVTQKPSAN
LATSWQVDDTGTVYTFDLKENVQFHTTEWFTPSRALTASDVAFSFNRILDKNNAFHNVNSGDYPWFNSINFSQMVTSVEA
ITPHKVRFTLSTPDNTFLSVLAASYAVIHSEEYAQQLAAIDEKTLIDVKPVGTGPFKLAQFQRSELVRLKRHEQYWQGPA
NMSQVVFDISSRGTGSLAKLLRQECDVVANPISSQLPIIIANDALTLYSQTAMNVAYIAINMREPILQDQRVRKALSFAI
DRSSLINSVYYNSGIEAKSILPPASWAYGNDSSHIRYDLNYAKGLLREAGVKDGLELTMWVPLDNNSYNPSPKKSAEIIQ
SDFAKLGIDLKILTSDILNPNELKDADVDLVLTGWNANSSDPDSLLRPLLSCDAKQAGFGVANWCNEEFDLLLDLAKETN
QPRYRINIYRQIQNVLSEELPVIPLAHGVKYQANQSSLTGFTLSPFNTYSFHNVVRVKELN

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=512, Percent_Identity=42.7734375, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789966, Length=531, Percent_Identity=30.8851224105461, Blast_Score=346, Evalue=2e-96,
Organism=Escherichia coli, GI1787052, Length=468, Percent_Identity=27.1367521367521, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1787762, Length=449, Percent_Identity=23.3853006681514, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789887, Length=560, Percent_Identity=21.9642857142857, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1787495, Length=559, Percent_Identity=23.0769230769231, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081878, Length=450, Percent_Identity=23.1111111111111, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789397, Length=523, Percent_Identity=22.1797323135755, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60276; Mature: 60276

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIARLLIAATGLFNLTACYDAGIKQTIHDTGFIYCESGNVDFFNPQISDGNSILEAIS
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEECCCEEEECCCCCCCHHHHHHHH
AQVYDRLLIIAPVTQKPSANLATSWQVDDTGTVYTFDLKENVQFHTTEWFTPSRALTASD
HHHHCEEEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCHHH
VAFSFNRILDKNNAFHNVNSGDYPWFNSINFSQMVTSVEAITPHKVRFTLSTPDNTFLSV
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHH
LAASYAVIHSEEYAQQLAAIDEKTLIDVKPVGTGPFKLAQFQRSELVRLKRHEQYWQGPA
HHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NMSQVVFDISSRGTGSLAKLLRQECDVVANPISSQLPIIIANDALTLYSQTAMNVAYIAI
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEHHHHHCEEEEEEE
NMREPILQDQRVRKALSFAIDRSSLINSVYYNSGIEAKSILPPASWAYGNDSSHIRYDLN
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEEEEECH
YAKGLLREAGVKDGLELTMWVPLDNNSYNPSPKKSAEIIQSDFAKLGIDLKILTSDILNP
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHCCCC
NELKDADVDLVLTGWNANSSDPDSLLRPLLSCDAKQAGFGVANWCNEEFDLLLDLAKETN
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHCC
QPRYRINIYRQIQNVLSEELPVIPLAHGVKYQANQSSLTGFTLSPFNTYSFHNVVRVKEL
CCEEEEHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCEEEECCCCCCCCCCEEEEEEC
N
C
>Mature Secondary Structure
MKIIARLLIAATGLFNLTACYDAGIKQTIHDTGFIYCESGNVDFFNPQISDGNSILEAIS
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEECCCEEEECCCCCCCHHHHHHHH
AQVYDRLLIIAPVTQKPSANLATSWQVDDTGTVYTFDLKENVQFHTTEWFTPSRALTASD
HHHHCEEEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCHHH
VAFSFNRILDKNNAFHNVNSGDYPWFNSINFSQMVTSVEAITPHKVRFTLSTPDNTFLSV
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHH
LAASYAVIHSEEYAQQLAAIDEKTLIDVKPVGTGPFKLAQFQRSELVRLKRHEQYWQGPA
HHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
NMSQVVFDISSRGTGSLAKLLRQECDVVANPISSQLPIIIANDALTLYSQTAMNVAYIAI
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEHHHHHCEEEEEEE
NMREPILQDQRVRKALSFAIDRSSLINSVYYNSGIEAKSILPPASWAYGNDSSHIRYDLN
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCEEEEECH
YAKGLLREAGVKDGLELTMWVPLDNNSYNPSPKKSAEIIQSDFAKLGIDLKILTSDILNP
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHCCCC
NELKDADVDLVLTGWNANSSDPDSLLRPLLSCDAKQAGFGVANWCNEEFDLLLDLAKETN
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHCC
QPRYRINIYRQIQNVLSEELPVIPLAHGVKYQANQSSLTGFTLSPFNTYSFHNVVRVKEL
CCEEEEHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCEEEECCCCCCCCCCEEEEEEC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]