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The map label for this gene is fmt

Identifier: 59713151

GI number: 59713151

Start: 2863770

End: 2864717

Strand: Direct

Name: fmt

Synonym: VF_2544

Alternate gene names: 59713151

Gene position: 2863770-2864717 (Clockwise)

Preceding gene: 59713150

Following gene: 59713152

Centisome position: 98.83

GC content: 43.14

Gene sequence:

>948_bases
TTGAGCAAACCATTACGCATTATTTTTGCGGGCACACCCGATTTCGCTGCTCGCCATTTATCAGCACTTATCGACTCTCA
CCATGAAGTGATTGGCGTATACACTCAACCTGATCGCCCAGCAGGTCGTGGTAAAAAGCTAACAGCAAGCCCAGTAAAAG
AGCTGGCGCTTGAGCACAATATTCCTGTATTCCAGCCTGAAAACTTTAAGAGTGATGAAGCAAAGCAAGAGCTTGTTGAC
CAAAATGCTGATTTAATGGTTGTGGTTGCTTATGGCTTACTGCTACCACAAGCGGTGTTAGATACACCAAAATTGGGTTG
CATTAACGTTCATGGCTCGATTCTTCCTCGTTGGCGTGGTGCTGCACCTATTCAACGCTCTATTTGGGCAGGTGACGCCG
AGACTGGCGTAACTATCATGCAAATGGATATTGGTTTAGATACTGGCGATATGCTGAAGATTGCAACTTTGCCTATCGAA
GCAACCGATACAAGCGCCTCAATGTACGACAAATTAGCTGAACTAGGCCCAGTCGCTTTAGTTGATTGTTTATCTGATAT
TGCTGATGGCTCTGCTATTGCACAAAAACAAGATGATGAACTGGCTAATTACGCGAAAAAACTCAGCAAAGAAGAAGCAA
AGATTGATTGGACAATGGATGCAATTGCTATTGAACGCTGTGTTCGTGCTTTTAATCCATGGCCAATGAGTCACTTCTCT
GTTGAAGATAAAGCCATTAAAGTATGGCAATCTCGTGTTGAAAGCTATACCGGAGATGCAACACCAGGCACCATTATTCA
AGCGGATAAAACGGGCATTTATGTTGCGACAGGTTCAGATGCCATTGTCTTTGAACAACTGCAAGTACCTGGAAAAAAAG
CAATGGGTGTTCAAGATATTTTGAATTCTCGCAAAGAATGGTTTGAAGTGGGTAATACCCTTAACTAA

Upstream 100 bases:

>100_bases
TTGATTACTTATCGCCTCTAAAGCGTAAGCGTATCAAAGAAAAACTAGAGAAAATCAAAAAATTCAACGCTAAAAATCAG
TAATTGATACCGAGGTTAAT

Downstream 100 bases:

>100_bases
TTCTTCAAAAATATAAAAAGGTGGCATAATTGCCACCTTCGTTTTTAAATGGCAAATATCGTATTTGCCTTTTTATTTTT
GCCAACCATATTTTAGGTGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSKPLRIIFAGTPDFAARHLSALIDSHHEVIGVYTQPDRPAGRGKKLTASPVKELALEHNIPVFQPENFKSDEAKQELVD
QNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIE
ATDTSASMYDKLAELGPVALVDCLSDIADGSAIAQKQDDELANYAKKLSKEEAKIDWTMDAIAIERCVRAFNPWPMSHFS
VEDKAIKVWQSRVESYTGDATPGTIIQADKTGIYVATGSDAIVFEQLQVPGKKAMGVQDILNSRKEWFEVGNTLN

Sequences:

>Translated_315_residues
MSKPLRIIFAGTPDFAARHLSALIDSHHEVIGVYTQPDRPAGRGKKLTASPVKELALEHNIPVFQPENFKSDEAKQELVD
QNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIE
ATDTSASMYDKLAELGPVALVDCLSDIADGSAIAQKQDDELANYAKKLSKEEAKIDWTMDAIAIERCVRAFNPWPMSHFS
VEDKAIKVWQSRVESYTGDATPGTIIQADKTGIYVATGSDAIVFEQLQVPGKKAMGVQDILNSRKEWFEVGNTLN
>Mature_314_residues
SKPLRIIFAGTPDFAARHLSALIDSHHEVIGVYTQPDRPAGRGKKLTASPVKELALEHNIPVFQPENFKSDEAKQELVDQ
NADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRGAAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIEA
TDTSASMYDKLAELGPVALVDCLSDIADGSAIAQKQDDELANYAKKLSKEEAKIDWTMDAIAIERCVRAFNPWPMSHFSV
EDKAIKVWQSRVESYTGDATPGTIIQADKTGIYVATGSDAIVFEQLQVPGKKAMGVQDILNSRKEWFEVGNTLN

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=222, Percent_Identity=33.7837837837838, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI238814322, Length=281, Percent_Identity=28.8256227758007, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI21614513, Length=284, Percent_Identity=28.8732394366197, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=63.3757961783439, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=27.4021352313167, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI133930964, Length=291, Percent_Identity=25.7731958762887, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550868, Length=304, Percent_Identity=30.2631578947368, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=32.5892857142857, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585660, Length=243, Percent_Identity=32.5102880658436, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_VIBF1 (Q5E1Q7)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205927.1
- ProteinModelPortal:   Q5E1Q7
- SMR:   Q5E1Q7
- STRING:   Q5E1Q7
- GeneID:   3279694
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2544
- NMPDR:   fig|312309.3.peg.2544
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q5E1Q7
- ProtClustDB:   PRK00005
- BioCyc:   VFIS312309:VF2544-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34371; Mature: 34239

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPLRIIFAGTPDFAARHLSALIDSHHEVIGVYTQPDRPAGRGKKLTASPVKELALEHN
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
IPVFQPENFKSDEAKQELVDQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRG
CCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIEATDTSASMYDKLAELGPVAL
CCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCHHHH
VDCLSDIADGSAIAQKQDDELANYAKKLSKEEAKIDWTMDAIAIERCVRAFNPWPMSHFS
HHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCCC
VEDKAIKVWQSRVESYTGDATPGTIIQADKTGIYVATGSDAIVFEQLQVPGKKAMGVQDI
CHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCEEEEHHHCCCCHHCCCHHHH
LNSRKEWFEVGNTLN
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKPLRIIFAGTPDFAARHLSALIDSHHEVIGVYTQPDRPAGRGKKLTASPVKELALEHN
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
IPVFQPENFKSDEAKQELVDQNADLMVVVAYGLLLPQAVLDTPKLGCINVHGSILPRWRG
CCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
AAPIQRSIWAGDAETGVTIMQMDIGLDTGDMLKIATLPIEATDTSASMYDKLAELGPVAL
CCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCHHHH
VDCLSDIADGSAIAQKQDDELANYAKKLSKEEAKIDWTMDAIAIERCVRAFNPWPMSHFS
HHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCCC
VEDKAIKVWQSRVESYTGDATPGTIIQADKTGIYVATGSDAIVFEQLQVPGKKAMGVQDI
CHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCEEEEHHHCCCCHHCCCHHHH
LNSRKEWFEVGNTLN
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA