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The map label for this gene is rsmB [H]

Identifier: 59713152

GI number: 59713152

Start: 2864822

End: 2866102

Strand: Direct

Name: rsmB [H]

Synonym: VF_2545

Alternate gene names: 59713152

Gene position: 2864822-2866102 (Clockwise)

Preceding gene: 59713151

Following gene: 59713153

Centisome position: 98.87

GC content: 42.23

Gene sequence:

>1281_bases
ATGAACGTTCGTGCTGCGGCAGCCAAAGTTATTTATCAAGTTGTCGATCAGGGTCATTCTCTCTCTTCAGCGCTTCCAAA
AGCTCAATCTGAGATCAAACCTCGTGACCAAGCTTTACTGCAAGAGATCTGCTATGGCGTACTGCGCTTTCTTCCTCGTT
TAGAGTCTGTTGCTCAATCATTAATGGATAAACCATTAAAGGGAAAACAACGAGTATTCCATCATCTAATTCTTGTGGGT
ATTTACCAACTGAGTTTCATGCGTATTCCTGCACACGCAGCAGTAGCGGAAACCGTTGAAGGTACAAAGAATTTAAAAGG
TCCAAAACTACGCGGACTGATCAACGCGGTATTACGTAATTATCAACGTAGCCAAGAAGAGTTGGATGCGAAATCAGTAA
GCCATGACTCAGGAAAATATGGTCATCCGGGCTGGTTATTGAAAATGCTACAACAAGCTTACCCAGAACAATGGGAAAAT
ATTGTTGAAGCCAATAACAGTAAAGCACCAATGTGGTTACGTGTTAATAGCCAACACCACACTCGCGATGAATATATAGC
TCTACTTGAAAAAGAAGAGATCAGTGTTACGCCACACAGCCAAGCGACGGATGCTCTGTTATTAGATAAAGCGCGTGATG
TATATACACTTCCTGGCTTTGAGCAAGGTTGGGTATCAGTACAAGATGCTGCTGCTCAATTATCAGCAGAGTACCTACAA
CCTCAAAATGGCGAATTAATTCTTGATTGCTGTGCTGCTCCTGGTGGTAAAACAGCGCACATTCTAGAACGCACACAAGA
TACAAGTGTGGTTGCCATTGATTGTGATGAAACACGTTTAAAACGAGTTTATGAAAACTTAGAACGCTTAAACCTTAAAG
CACAAGTTATTTGTGGTGATGCTCGCCATCCAGAAGAATGGTGGAAAGGCGAACAATTTGACCGCATTTTATTGGACGCA
CCTTGTTCTGCAACAGGGGTTATTCGTCGTCACCCAGACATCAAGTGGTTACGTCGTTCAGAAGACATTAAAGCACTGGC
TGAACTGCAAAGTGAAATCTTTGATGCTATGTGGTTACAGTTAAAGTCTGGCGGCACCATGGTTTATGCGACTTGTTCAA
TTACGCCTTTAGAAAACTGCGATCAGGTAAAAGCGTTTTTAGCACGAACAAGTGATGCAACATTAATTGATAATACAGAT
CCTGAAAAACCAGGACGTCAAATTCTTCCAGGTGAAGAGAATATGGACGGCTTCTACTACGCTGTTCTACAAAAAGCCTA
A

Upstream 100 bases:

>100_bases
TCAAAAATATAAAAAGGTGGCATAATTGCCACCTTCGTTTTTAAATGGCAAATATCGTATTTGCCTTTTTATTTTTGCCA
ACCATATTTTAGGTGATGAC

Downstream 100 bases:

>100_bases
TCGGAATACGAGCGAGCAAACGGTATGAAAATCATCATCTTAGGTGCGGGTCAAGTGGGCGGCACATTAGCTGAGAACTT
GGTGGGCGAAAACAACGATA

Product: 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQSLMDKPLKGKQRVFHHLILVG
IYQLSFMRIPAHAAVAETVEGTKNLKGPKLRGLINAVLRNYQRSQEELDAKSVSHDSGKYGHPGWLLKMLQQAYPEQWEN
IVEANNSKAPMWLRVNSQHHTRDEYIALLEKEEISVTPHSQATDALLLDKARDVYTLPGFEQGWVSVQDAAAQLSAEYLQ
PQNGELILDCCAAPGGKTAHILERTQDTSVVAIDCDETRLKRVYENLERLNLKAQVICGDARHPEEWWKGEQFDRILLDA
PCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQLKSGGTMVYATCSITPLENCDQVKAFLARTSDATLIDNTD
PEKPGRQILPGEENMDGFYYAVLQKA

Sequences:

>Translated_426_residues
MNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQSLMDKPLKGKQRVFHHLILVG
IYQLSFMRIPAHAAVAETVEGTKNLKGPKLRGLINAVLRNYQRSQEELDAKSVSHDSGKYGHPGWLLKMLQQAYPEQWEN
IVEANNSKAPMWLRVNSQHHTRDEYIALLEKEEISVTPHSQATDALLLDKARDVYTLPGFEQGWVSVQDAAAQLSAEYLQ
PQNGELILDCCAAPGGKTAHILERTQDTSVVAIDCDETRLKRVYENLERLNLKAQVICGDARHPEEWWKGEQFDRILLDA
PCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQLKSGGTMVYATCSITPLENCDQVKAFLARTSDATLIDNTD
PEKPGRQILPGEENMDGFYYAVLQKA
>Mature_426_residues
MNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQSLMDKPLKGKQRVFHHLILVG
IYQLSFMRIPAHAAVAETVEGTKNLKGPKLRGLINAVLRNYQRSQEELDAKSVSHDSGKYGHPGWLLKMLQQAYPEQWEN
IVEANNSKAPMWLRVNSQHHTRDEYIALLEKEEISVTPHSQATDALLLDKARDVYTLPGFEQGWVSVQDAAAQLSAEYLQ
PQNGELILDCCAAPGGKTAHILERTQDTSVVAIDCDETRLKRVYENLERLNLKAQVICGDARHPEEWWKGEQFDRILLDA
PCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQLKSGGTMVYATCSITPLENCDQVKAFLARTSDATLIDNTD
PEKPGRQILPGEENMDGFYYAVLQKA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=343, Percent_Identity=31.1953352769679, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI76150623, Length=343, Percent_Identity=31.1953352769679, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI32698918, Length=186, Percent_Identity=34.9462365591398, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI301336155, Length=319, Percent_Identity=26.3322884012539, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI39995082, Length=345, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI8922322, Length=301, Percent_Identity=25.9136212624585, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI270288818, Length=301, Percent_Identity=25.9136212624585, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI270288816, Length=301, Percent_Identity=25.9136212624585, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI23199998, Length=301, Percent_Identity=25.9136212624585, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316918, Length=162, Percent_Identity=32.0987654320988, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11545785, Length=189, Percent_Identity=29.1005291005291, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=424, Percent_Identity=59.1981132075472, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI87081985, Length=310, Percent_Identity=24.5161290322581, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=312, Percent_Identity=29.8076923076923, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25143471, Length=189, Percent_Identity=35.4497354497354, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71998419, Length=300, Percent_Identity=26, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=264, Percent_Identity=26.5151515151515, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=370, Percent_Identity=28.3783783783784, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319447, Length=199, Percent_Identity=32.6633165829146, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI22024126, Length=311, Percent_Identity=28.2958199356913, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19920494, Length=165, Percent_Identity=32.7272727272727, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=28.3653846153846, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47892; Mature: 47892

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQS
CCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMDKPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVAETVEGTKNLKGPKLRGLINAVLRN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YQRSQEELDAKSVSHDSGKYGHPGWLLKMLQQAYPEQWENIVEANNSKAPMWLRVNSQHH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCCCC
TRDEYIALLEKEEISVTPHSQATDALLLDKARDVYTLPGFEQGWVSVQDAAAQLSAEYLQ
CHHHHHHHHHHCCCEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHC
PQNGELILDCCAAPGGKTAHILERTQDTSVVAIDCDETRLKRVYENLERLNLKAQVICGD
CCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEECC
ARHPEEWWKGEQFDRILLDAPCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQ
CCCCHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSGGTMVYATCSITPLENCDQVKAFLARTSDATLIDNTDPEKPGRQILPGEENMDGFYY
EECCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCEEE
AVLQKA
EEEECC
>Mature Secondary Structure
MNVRAAAAKVIYQVVDQGHSLSSALPKAQSEIKPRDQALLQEICYGVLRFLPRLESVAQS
CCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMDKPLKGKQRVFHHLILVGIYQLSFMRIPAHAAVAETVEGTKNLKGPKLRGLINAVLRN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
YQRSQEELDAKSVSHDSGKYGHPGWLLKMLQQAYPEQWENIVEANNSKAPMWLRVNSQHH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCCCC
TRDEYIALLEKEEISVTPHSQATDALLLDKARDVYTLPGFEQGWVSVQDAAAQLSAEYLQ
CHHHHHHHHHHCCCEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHC
PQNGELILDCCAAPGGKTAHILERTQDTSVVAIDCDETRLKRVYENLERLNLKAQVICGD
CCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEECC
ARHPEEWWKGEQFDRILLDAPCSATGVIRRHPDIKWLRRSEDIKALAELQSEIFDAMWLQ
CCCCHHHHCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKSGGTMVYATCSITPLENCDQVKAFLARTSDATLIDNTDPEKPGRQILPGEENMDGFYY
EECCCEEEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCEEE
AVLQKA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]