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Identifier: 59713150

GI number: 59713150

Start: 2863240

End: 2863752

Strand: Direct

Name: def

Synonym: VF_2543

Alternate gene names: 59713150

Gene position: 2863240-2863752 (Clockwise)

Preceding gene: 59713145

Following gene: 59713151

Centisome position: 98.82

GC content: 39.96

Gene sequence:

>513_bases
ATGGCTTTATTAGAAGTATTAACGTTCCCGGATGACCGTCTACGTACCGTAGCAAAACCGGTAGAAGCAGTAACACCTGA
AATCCAAAAATTTGTCGATGACATGATTGAAACCATGTACGACGAAGAAGGTATTGGCCTTGCTGCTACACAAGTCGATT
TTCACCAACGTATCGTTGTGATTGATGTTTCTGAAACTCGTGACGAACCAATGGTTTTAATCAATCCTGTTATCACACAA
AAAAGTGGTGATGATGGTATTGAAGAAGGCTGTTTGTCTGTTCCTGGAGCAAAAGGACTTGTTCCTCGCTCAGCAGAGGT
ATCAGTTAGCGCTCTTGATCGCGATGGTAATGAATTTAGCTTTGATGCTGATGATTTATTAGCTATCTGTGTTCAGCACG
AACTGGATCACCTTGATGGCAAGTTATTCGTTGATTACTTATCGCCTCTAAAGCGTAAGCGTATCAAAGAAAAACTAGAG
AAAATCAAAAAATTCAACGCTAAAAATCAGTAA

Upstream 100 bases:

>100_bases
AGACATACCAACCGATGCTGTCATTTGCTCAATAAAATGTCTAGAATTGGAACTATTAGGTTTATGCCGTTTTCGGCGCA
GTTCAACATTTTGAGTATTT

Downstream 100 bases:

>100_bases
TTGATACCGAGGTTAATTTGAGCAAACCATTACGCATTATTTTTGCGGGCACACCCGATTTCGCTGCTCGCCATTTATCA
GCACTTATCGACTCTCACCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MALLEVLTFPDDRLRTVAKPVEAVTPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVVIDVSETRDEPMVLINPVITQ
KSGDDGIEEGCLSVPGAKGLVPRSAEVSVSALDRDGNEFSFDADDLLAICVQHELDHLDGKLFVDYLSPLKRKRIKEKLE
KIKKFNAKNQ

Sequences:

>Translated_170_residues
MALLEVLTFPDDRLRTVAKPVEAVTPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVVIDVSETRDEPMVLINPVITQ
KSGDDGIEEGCLSVPGAKGLVPRSAEVSVSALDRDGNEFSFDADDLLAICVQHELDHLDGKLFVDYLSPLKRKRIKEKLE
KIKKFNAKNQ
>Mature_169_residues
ALLEVLTFPDDRLRTVAKPVEAVTPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVVIDVSETRDEPMVLINPVITQK
SGDDGIEEGCLSVPGAKGLVPRSAEVSVSALDRDGNEFSFDADDLLAICVQHELDHLDGKLFVDYLSPLKRKRIKEKLEK
IKKFNAKNQ

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=31.2101910828025, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=66.0714285714286, Blast_Score=239, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24645726, Length=141, Percent_Identity=29.7872340425532, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_VIBF1 (Q5E1Q8)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205926.1
- HSSP:   P0A6K3
- ProteinModelPortal:   Q5E1Q8
- SMR:   Q5E1Q8
- STRING:   Q5E1Q8
- GeneID:   3279717
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2543
- NMPDR:   fig|312309.3.peg.2543
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- PhylomeDB:   Q5E1Q8
- ProtClustDB:   PRK00150
- BioCyc:   VFIS312309:VF2543-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 18997; Mature: 18866

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEVLTFPDDRLRTVAKPVEAVTPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVV
CCHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEE
IDVSETRDEPMVLINPVITQKSGDDGIEEGCLSVPGAKGLVPRSAEVSVSALDRDGNEFS
EECCCCCCCCEEEECCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
FDADDLLAICVQHELDHLDGKLFVDYLSPLKRKRIKEKLEKIKKFNAKNQ
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALLEVLTFPDDRLRTVAKPVEAVTPEIQKFVDDMIETMYDEEGIGLAATQVDFHQRIVV
CHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEE
IDVSETRDEPMVLINPVITQKSGDDGIEEGCLSVPGAKGLVPRSAEVSVSALDRDGNEFS
EECCCCCCCCEEEECCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCC
FDADDLLAICVQHELDHLDGKLFVDYLSPLKRKRIKEKLEKIKKFNAKNQ
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA