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The map label for this gene is purK

Identifier: 59713145

GI number: 59713145

Start: 2858728

End: 2859840

Strand: Direct

Name: purK

Synonym: VF_2538

Alternate gene names: 59713145

Gene position: 2858728-2859840 (Clockwise)

Preceding gene: 59713144

Following gene: 59713150

Centisome position: 98.66

GC content: 37.2

Gene sequence:

>1113_bases
ATGAATGTTCTTATTTTAGGTTCAGGTCAATTAGCACGAATGATGTCTTTAGCTAGTGCTCCATTAAATATTGATGTGAT
CGCTTATGATGTTAATAGCCAACAAATTGTACATCCATTAACTCAACAACCGATTGATTTATCATTAGAACAAGCCATTA
AACAAGTCGATGTTATTACGGCTGAGTTTGAACATATTCCTCATCATATTCTAGATATTTGTAGTGTAAGTGGAAAATTT
CAACCAACACCTGATGCAATAAAAGCAGGTGGTGATCGCCGTCTTGAAAAAAAGTTATTAGATAACGCGCATGTTGCAAA
TGCCAAATACCATAATATCAATACTAAACAGGACTTTTTGAATGCCATCGATACCGTTGGGATCCCTATGGTATTGAAGA
GTGCGTTAGGCGGTTATGATGGAAAAGGTCAATGGCGCTTAAAAGACGTATCACAAGTTGATTCTATTTGGGAAGAAATG
GATGAGTTAATTAAAAGCTCTCCAAATCAAGCAATAGTTGCAGAACAATTTATTCCATTTAACCGTGAAGTTTCACTCAT
TGGAGCTCGTAATAAAAACGGTCAAATGGTGACTTACCCACTGACTGAAAATGTTCATGTCGATGGTGTTCTTGCTTTAT
CAACCGCTTTAGAAGGGACAAGTAATTTACAACAACAAGCCGATGAAATGTTTACAGCTATCGCTGATGAACTGAATTAT
ATCGGAGTGCTTGCTATTGAGTTTTTCGATGTAGACGGAACGTTACTTGTAAATGAAATTGCCCCTCGTGTGCATAATTC
AGGGCACTGGACTCAACAAGGGACCGATATTTGCCAATTTGAAATGCACATTCGAGCGGTATGCAACTTACCATTAACGG
GTAATCAATTACTTCGCTCAACTGCGATGATTAATATTTTAGGTGAAGATAGCTTACCAGATGAAATATTAACAATGCCG
AATTGCCATATTCATTGGTATGGAAAAGAGAAGCGAGCGGGTAGAAAAATGGGACATATTAACGTGTGTGCCGACTCCCC
TGAAATGCTACAAAAAGAGCTTTCTAAACTTGCAAATATTTTAGATAAACAAGCCTTTAAAGCTCTTTCTTAA

Upstream 100 bases:

>100_bases
TTGGAACATCAAACGAAGAAGTAATGGCTGCGGTTGAAGCTTTCCGTAAAGAACAAACTGAAATGGTGTTAGAGAACCCA
GATCCATCAGAGGATTAATC

Downstream 100 bases:

>100_bases
ATCTCAAGGTATGAGTAACTTAATACTTGTGATTAAGTTACTCTGATTGGCTCTGAACTTCTGCACATTTTTTATCTGCA
CAGATTAAACGAGTTCCTGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNVLILGSGQLARMMSLASAPLNIDVIAYDVNSQQIVHPLTQQPIDLSLEQAIKQVDVITAEFEHIPHHILDICSVSGKF
QPTPDAIKAGGDRRLEKKLLDNAHVANAKYHNINTKQDFLNAIDTVGIPMVLKSALGGYDGKGQWRLKDVSQVDSIWEEM
DELIKSSPNQAIVAEQFIPFNREVSLIGARNKNGQMVTYPLTENVHVDGVLALSTALEGTSNLQQQADEMFTAIADELNY
IGVLAIEFFDVDGTLLVNEIAPRVHNSGHWTQQGTDICQFEMHIRAVCNLPLTGNQLLRSTAMINILGEDSLPDEILTMP
NCHIHWYGKEKRAGRKMGHINVCADSPEMLQKELSKLANILDKQAFKALS

Sequences:

>Translated_370_residues
MNVLILGSGQLARMMSLASAPLNIDVIAYDVNSQQIVHPLTQQPIDLSLEQAIKQVDVITAEFEHIPHHILDICSVSGKF
QPTPDAIKAGGDRRLEKKLLDNAHVANAKYHNINTKQDFLNAIDTVGIPMVLKSALGGYDGKGQWRLKDVSQVDSIWEEM
DELIKSSPNQAIVAEQFIPFNREVSLIGARNKNGQMVTYPLTENVHVDGVLALSTALEGTSNLQQQADEMFTAIADELNY
IGVLAIEFFDVDGTLLVNEIAPRVHNSGHWTQQGTDICQFEMHIRAVCNLPLTGNQLLRSTAMINILGEDSLPDEILTMP
NCHIHWYGKEKRAGRKMGHINVCADSPEMLQKELSKLANILDKQAFKALS
>Mature_370_residues
MNVLILGSGQLARMMSLASAPLNIDVIAYDVNSQQIVHPLTQQPIDLSLEQAIKQVDVITAEFEHIPHHILDICSVSGKF
QPTPDAIKAGGDRRLEKKLLDNAHVANAKYHNINTKQDFLNAIDTVGIPMVLKSALGGYDGKGQWRLKDVSQVDSIWEEM
DELIKSSPNQAIVAEQFIPFNREVSLIGARNKNGQMVTYPLTENVHVDGVLALSTALEGTSNLQQQADEMFTAIADELNY
IGVLAIEFFDVDGTLLVNEIAPRVHNSGHWTQQGTDICQFEMHIRAVCNLPLTGNQLLRSTAMINILGEDSLPDEILTMP
NCHIHWYGKEKRAGRKMGHINVCADSPEMLQKELSKLANILDKQAFKALS

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=363, Percent_Identity=38.0165289256198, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788155, Length=367, Percent_Identity=23.1607629427793, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=389, Percent_Identity=30.8483290488432, Blast_Score=168, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_VIBF1 (Q5E1R3)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205921.1
- ProteinModelPortal:   Q5E1R3
- SMR:   Q5E1R3
- STRING:   Q5E1R3
- GeneID:   3279691
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2538
- NMPDR:   fig|312309.3.peg.2538
- eggNOG:   COG0026
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- PhylomeDB:   Q5E1R3
- ProtClustDB:   PRK06019
- BioCyc:   VFIS312309:VF2538-MONOMER
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 40933; Mature: 40933

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 93-93 BINDING 133-133 BINDING 144-144 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLILGSGQLARMMSLASAPLNIDVIAYDVNSQQIVHPLTQQPIDLSLEQAIKQVDVIT
CEEEEECCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
AEFEHIPHHILDICSVSGKFQPTPDAIKAGGDRRLEKKLLDNAHVANAKYHNINTKQDFL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCHHHHH
NAIDTVGIPMVLKSALGGYDGKGQWRLKDVSQVDSIWEEMDELIKSSPNQAIVAEQFIPF
HHHHHCCHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCC
NREVSLIGARNKNGQMVTYPLTENVHVDGVLALSTALEGTSNLQQQADEMFTAIADELNY
CCCEEEEEECCCCCCEEEEECCCCCEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
IGVLAIEFFDVDGTLLVNEIAPRVHNSGHWTQQGTDICQFEMHIRAVCNLPLTGNQLLRS
EEEEEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHEEHHHCCCCCCHHHHHHH
TAMINILGEDSLPDEILTMPNCHIHWYGKEKRAGRKMGHINVCADSPEMLQKELSKLANI
HHEEEEECCCCCCHHHHCCCCCEEEEECCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
LDKQAFKALS
HHHHHHHHCC
>Mature Secondary Structure
MNVLILGSGQLARMMSLASAPLNIDVIAYDVNSQQIVHPLTQQPIDLSLEQAIKQVDVIT
CEEEEECCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
AEFEHIPHHILDICSVSGKFQPTPDAIKAGGDRRLEKKLLDNAHVANAKYHNINTKQDFL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCHHHHH
NAIDTVGIPMVLKSALGGYDGKGQWRLKDVSQVDSIWEEMDELIKSSPNQAIVAEQFIPF
HHHHHCCHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCC
NREVSLIGARNKNGQMVTYPLTENVHVDGVLALSTALEGTSNLQQQADEMFTAIADELNY
CCCEEEEEECCCCCCEEEEECCCCCEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
IGVLAIEFFDVDGTLLVNEIAPRVHNSGHWTQQGTDICQFEMHIRAVCNLPLTGNQLLRS
EEEEEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHEEHHHCCCCCCHHHHHHH
TAMINILGEDSLPDEILTMPNCHIHWYGKEKRAGRKMGHINVCADSPEMLQKELSKLANI
HHEEEEECCCCCCHHHHCCCCCEEEEECCHHHCCCCCCEEEEECCCHHHHHHHHHHHHHH
LDKQAFKALS
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA