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The map label for this gene is dcuA [H]

Identifier: 59712961

GI number: 59712961

Start: 2641852

End: 2643153

Strand: Direct

Name: dcuA [H]

Synonym: VF_2354

Alternate gene names: 59712961

Gene position: 2641852-2643153 (Clockwise)

Preceding gene: 59712960

Following gene: 59712963

Centisome position: 91.18

GC content: 42.55

Gene sequence:

>1302_bases
ATGATTGCTGTACAACTTTTCGTAGTACTGCTGTTCATCTATTTAGGTGCGCGCATTGGGGGTATTGGTATTGGTTTTGC
GGGCGGCGCAGGTGTACTAGTTCTAACCATGATTTTAGGTCTAGACGCTGGTTCTATCCCTATCGATGTTATCCTAATTA
TCATGTCAGTTATTACTGCTATTGCAGCGATGCAAGTTGCTGGTGGTATGGATTGGCTAGTTGACCTAGCAGAAAAATTC
TTACGTAAAAATCCAAAACGCATTACTTTCTATGCACCTATCGTAACGTATTTCATGACAATGCTAGCTGGTACTGGCCA
CACGGCTTTCTCTACTCTTCCAGTTATTGCTGAAGTTGCAAAAGAACAAGGTGTTCGTCCATCTCGCCCACTTTCTATCG
CCGTTGTTGCATCTCAAATTGCAATCACAGCGTCTCCTATTTCAGCGGCGGTTGTTTTCTTCTCAGGTATCCTCGAGCCT
CTAGGTGTGGATTACTTAACGCTATTAGCTGTATGTATTCCTTCAACATTCCTAGCATGTATGGTTGGTGCTTTTGTTGC
TAACTTCCTAGGTTGTGAACTAAAAGATGATTCTGTTTATCAAGAGCGCCTTCAAAAAGGCCTAATTAAGATGAATGGCA
CAGGCAAGCGTGAAATTTTACCAACAGCAAAAGCATCAGTTTACATCTTCTGTGCGGCAATCATTGCTGTTGTGGCTTAC
GCTACAATGATCAGTGGTAGTGTGGGCCTAATTGAGCACCCAACCATTGGTCGTAACGAAGCTATCATGGCTCTAATGCT
AACGGCTGCTGCTGCAATTGTTACTTTCACTAAAATTGATGCGTCTAAAATTGCTAACGCAGCAACATTTAAATCAGGTA
TGAGTGCTTGTGTATGTGTGCTTGGTGTTGCTTGGTTAGGTGATACATTTGTATCTTCTCACATTGGTGAAATCAAAGAA
TTCTCTGCACACATTCTTGAACAATACCCTTGGATGCTAGCTGTTGTTTTATTCTTTGCTTCAATGCTTCTTTATTCTCA
AGGTGCAACAACAACAGCTCTAATGCCTGCTGCTCTTGCTATCGGTGTTGCACCAATCACAGCTGTTGCTTCTTTTGCTG
CGGTAAGTGCACTGTTCGTTCTTCCAACTTACCCAACACTACTTGCAGCGGTTGAGATGGATGACACAGGTTCAACTCGT
ATCGGTAACCTAGTATTTAACCACCCATTCTTTATCCCTGGTGTGGCAACTATCGCAACATCTGTTGCTCTAGGTTTTGC
ATTCGGTGGTATTATTCTTTAA

Upstream 100 bases:

>100_bases
AAATACTCAAAGTAATTATTCATATTTTTCCCAATTACTATATATAGCTACTCAGTTAAAATATGAATAATTATTTAATA
TTAAAAAAGTGGAGTTTCAT

Downstream 100 bases:

>100_bases
TAGGTGAATTGCAATAATATCCCTCAAAATGGCAGCTGATTAAGCTGCCATTTTTTTATGTCTTTACGAAGGGATTGGAA
CAACCAAAATATCGACAGGT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIAVQLFVVLLFIYLGARIGGIGIGFAGGAGVLVLTMILGLDAGSIPIDVILIIMSVITAIAAMQVAGGMDWLVDLAEKF
LRKNPKRITFYAPIVTYFMTMLAGTGHTAFSTLPVIAEVAKEQGVRPSRPLSIAVVASQIAITASPISAAVVFFSGILEP
LGVDYLTLLAVCIPSTFLACMVGAFVANFLGCELKDDSVYQERLQKGLIKMNGTGKREILPTAKASVYIFCAAIIAVVAY
ATMISGSVGLIEHPTIGRNEAIMALMLTAAAAIVTFTKIDASKIANAATFKSGMSACVCVLGVAWLGDTFVSSHIGEIKE
FSAHILEQYPWMLAVVLFFASMLLYSQGATTTALMPAALAIGVAPITAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTR
IGNLVFNHPFFIPGVATIATSVALGFAFGGIIL

Sequences:

>Translated_433_residues
MIAVQLFVVLLFIYLGARIGGIGIGFAGGAGVLVLTMILGLDAGSIPIDVILIIMSVITAIAAMQVAGGMDWLVDLAEKF
LRKNPKRITFYAPIVTYFMTMLAGTGHTAFSTLPVIAEVAKEQGVRPSRPLSIAVVASQIAITASPISAAVVFFSGILEP
LGVDYLTLLAVCIPSTFLACMVGAFVANFLGCELKDDSVYQERLQKGLIKMNGTGKREILPTAKASVYIFCAAIIAVVAY
ATMISGSVGLIEHPTIGRNEAIMALMLTAAAAIVTFTKIDASKIANAATFKSGMSACVCVLGVAWLGDTFVSSHIGEIKE
FSAHILEQYPWMLAVVLFFASMLLYSQGATTTALMPAALAIGVAPITAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTR
IGNLVFNHPFFIPGVATIATSVALGFAFGGIIL
>Mature_433_residues
MIAVQLFVVLLFIYLGARIGGIGIGFAGGAGVLVLTMILGLDAGSIPIDVILIIMSVITAIAAMQVAGGMDWLVDLAEKF
LRKNPKRITFYAPIVTYFMTMLAGTGHTAFSTLPVIAEVAKEQGVRPSRPLSIAVVASQIAITASPISAAVVFFSGILEP
LGVDYLTLLAVCIPSTFLACMVGAFVANFLGCELKDDSVYQERLQKGLIKMNGTGKREILPTAKASVYIFCAAIIAVVAY
ATMISGSVGLIEHPTIGRNEAIMALMLTAAAAIVTFTKIDASKIANAATFKSGMSACVCVLGVAWLGDTFVSSHIGEIKE
FSAHILEQYPWMLAVVLFFASMLLYSQGATTTALMPAALAIGVAPITAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTR
IGNLVFNHPFFIPGVATIATSVALGFAFGGIIL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=434, Percent_Identity=58.9861751152074, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1790565, Length=407, Percent_Identity=36.1179361179361, Blast_Score=235, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45251; Mature: 45251

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVQLFVVLLFIYLGARIGGIGIGFAGGAGVLVLTMILGLDAGSIPIDVILIIMSVITA
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IAAMQVAGGMDWLVDLAEKFLRKNPKRITFYAPIVTYFMTMLAGTGHTAFSTLPVIAEVA
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KEQGVRPSRPLSIAVVASQIAITASPISAAVVFFSGILEPLGVDYLTLLAVCIPSTFLAC
HHCCCCCCCCEEEEEEHHHHHEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
MVGAFVANFLGCELKDDSVYQERLQKGLIKMNGTGKREILPTAKASVYIFCAAIIAVVAY
HHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ATMISGSVGLIEHPTIGRNEAIMALMLTAAAAIVTFTKIDASKIANAATFKSGMSACVCV
HHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LGVAWLGDTFVSSHIGEIKEFSAHILEQYPWMLAVVLFFASMLLYSQGATTTALMPAALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IGVAPITAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGNLVFNHPFFIPGVATIAT
HHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCHHHCCCCCCCCCHHHHHH
SVALGFAFGGIIL
HHHHHHHHHHHCC
>Mature Secondary Structure
MIAVQLFVVLLFIYLGARIGGIGIGFAGGAGVLVLTMILGLDAGSIPIDVILIIMSVITA
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IAAMQVAGGMDWLVDLAEKFLRKNPKRITFYAPIVTYFMTMLAGTGHTAFSTLPVIAEVA
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KEQGVRPSRPLSIAVVASQIAITASPISAAVVFFSGILEPLGVDYLTLLAVCIPSTFLAC
HHCCCCCCCCEEEEEEHHHHHEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
MVGAFVANFLGCELKDDSVYQERLQKGLIKMNGTGKREILPTAKASVYIFCAAIIAVVAY
HHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ATMISGSVGLIEHPTIGRNEAIMALMLTAAAAIVTFTKIDASKIANAATFKSGMSACVCV
HHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LGVAWLGDTFVSSHIGEIKEFSAHILEQYPWMLAVVLFFASMLLYSQGATTTALMPAALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IGVAPITAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGNLVFNHPFFIPGVATIAT
HHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCHHHCCCCCCCCCHHHHHH
SVALGFAFGGIIL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]