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The map label for this gene is aspA [H]

Identifier: 59712960

GI number: 59712960

Start: 2640236

End: 2641687

Strand: Direct

Name: aspA [H]

Synonym: VF_2353

Alternate gene names: 59712960

Gene position: 2640236-2641687 (Clockwise)

Preceding gene: 59712958

Following gene: 59712961

Centisome position: 91.12

GC content: 41.25

Gene sequence:

>1452_bases
ATGACTCAGATGCTTGATTTAGATACAAAGAATACTGCAGCAACTCGTATTGAAGAAGATTTGCTAGGTGAACGTCACGT
TCCTGCTGAAGCTTACTACGGTATTCACACTCTTCGTGCTATCGAAAACTTCAATATTTCTAATGTAACAATTTCTGATG
TTCCAGAATTTGTACGCGGCATGATCTACACTAAAAAAGCGGCGGCACTAGCGAACAAAGAATTAGGCGCTATTCCTTCA
CAAGTTGGTGAATACATCATTAAAGCATGTGACCTTATCCTTGAAACAGGTAAGTGTATGGATCAATTCCCATCTGACGT
TTTCCAAGGTGGTGCTGGTACTTCTGTTAACATGAACGCTAACGAAGTTATCGCTAACGTTGCTCTTGAATTAATGGGCA
AAGAAAAAGGCGAATACGACATCGTTAACCCTAACGATCATGTAAACAAATCTCAATCAACTAACTGTGCATACCCTACA
GGTTTCCGTATTGCGGTATACAACAGCATGATGAAACTGATCGAAGCGATTGAATACCTAAAAGGTGCATTCGATCTTAA
AGATCAAGAATTCCGTAACATTCTGAAAATGGGTCGTACTCAACTTCAAGATGCGGTTCCAATGACTGTTGGTCAAGAAT
TCCACGCTTGGGGTGTATTACTTCAAGAGGAAGTGAAAAACCTTCACTACACAGCACAACTTCTATTAGAAGTGAACATT
GGTGCGACAGCAATCGGTACTGGCTTAAACGCTGCGACTGGTTACCAAGAAGCAGCAGTTAAACATCTATCTGAAGTAAC
AGGTCTACCATGTGTTGCTGCTGAAGACTTAATCGAAGCTACATCTGACTGTGGTGCTTACGTAATGATCCACGGTGCGC
TAAAACGTACTGCGGTTAAGATGTCTAAGATCTGTAATGACTTACGCCTTCTTTCTTCTGGTCCTCGTGCTGGTCTAAAT
GAATTGAACCTTCCAGAGCTTCAAGCTGGTTCTTCAATCATGCCTGCAAAAGTTAACCCAGTAATCCCAGAAGTAGTTAA
CCAAGTGTGCTTCAAAGTAATCGGTAACGATACTACTGTTACTTTTGCTGCTGAAGCGGGTCAATTACAACTAAACGTAA
TGGAACCTGTTATTGGTCAAGCTCTGTTTGAATCAATCGACATCCTACGTAATGCATGTGTGAACCTACGCGATAAGTGT
ATTGATGGTATCACTGTAAACAAAGAAGTATGTGAAGCTCACGTATTTAACTCTATCGGTATCGTAACTTACCTAAACCC
ATTCATTGGCCACCACGAAGGTGACATTGTTGGTAAAATCTGTGCTGAAACAGGTAAGAGTGTTCGTGAAGTAGTTCTAG
AACGCGGTCTACTGACTGAAGAACAGCTGGACGACATCTTCTCTGTTGAAAACCTGATGCACCCAGAATACAAAGCAAAA
CGTTACGATTAA

Upstream 100 bases:

>100_bases
CAGGTACGATATTGATCAACTCTGACGAACGGACTCTACTCGCAGATATGATCACTTGGAAAGAATCCAATAATTAAATA
TAGAACCTTAAAGGCTTACT

Downstream 100 bases:

>100_bases
TTCTTAACGTTCAATAGCGAGCTCTCAGGGGCTCGCTTTTTTTATTTGTAATTTCTTATTTATAAAATACTCAAAGTAAT
TATTCATATTTTTCCCAATT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTQMLDLDTKNTAATRIEEDLLGERHVPAEAYYGIHTLRAIENFNISNVTISDVPEFVRGMIYTKKAAALANKELGAIPS
QVGEYIIKACDLILETGKCMDQFPSDVFQGGAGTSVNMNANEVIANVALELMGKEKGEYDIVNPNDHVNKSQSTNCAYPT
GFRIAVYNSMMKLIEAIEYLKGAFDLKDQEFRNILKMGRTQLQDAVPMTVGQEFHAWGVLLQEEVKNLHYTAQLLLEVNI
GATAIGTGLNAATGYQEAAVKHLSEVTGLPCVAAEDLIEATSDCGAYVMIHGALKRTAVKMSKICNDLRLLSSGPRAGLN
ELNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQALFESIDILRNACVNLRDKC
IDGITVNKEVCEAHVFNSIGIVTYLNPFIGHHEGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSVENLMHPEYKAK
RYD

Sequences:

>Translated_483_residues
MTQMLDLDTKNTAATRIEEDLLGERHVPAEAYYGIHTLRAIENFNISNVTISDVPEFVRGMIYTKKAAALANKELGAIPS
QVGEYIIKACDLILETGKCMDQFPSDVFQGGAGTSVNMNANEVIANVALELMGKEKGEYDIVNPNDHVNKSQSTNCAYPT
GFRIAVYNSMMKLIEAIEYLKGAFDLKDQEFRNILKMGRTQLQDAVPMTVGQEFHAWGVLLQEEVKNLHYTAQLLLEVNI
GATAIGTGLNAATGYQEAAVKHLSEVTGLPCVAAEDLIEATSDCGAYVMIHGALKRTAVKMSKICNDLRLLSSGPRAGLN
ELNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQALFESIDILRNACVNLRDKC
IDGITVNKEVCEAHVFNSIGIVTYLNPFIGHHEGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSVENLMHPEYKAK
RYD
>Mature_482_residues
TQMLDLDTKNTAATRIEEDLLGERHVPAEAYYGIHTLRAIENFNISNVTISDVPEFVRGMIYTKKAAALANKELGAIPSQ
VGEYIIKACDLILETGKCMDQFPSDVFQGGAGTSVNMNANEVIANVALELMGKEKGEYDIVNPNDHVNKSQSTNCAYPTG
FRIAVYNSMMKLIEAIEYLKGAFDLKDQEFRNILKMGRTQLQDAVPMTVGQEFHAWGVLLQEEVKNLHYTAQLLLEVNIG
ATAIGTGLNAATGYQEAAVKHLSEVTGLPCVAAEDLIEATSDCGAYVMIHGALKRTAVKMSKICNDLRLLSSGPRAGLNE
LNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQALFESIDILRNACVNLRDKCI
DGITVNKEVCEAHVFNSIGIVTYLNPFIGHHEGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSVENLMHPEYKAKR
YD

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=39.6103896103896, Blast_Score=328, Evalue=1e-89,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=79.2291220556745, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=36.6013071895425, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=40.4301075268817, Blast_Score=341, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.0611620795107, Blast_Score=247, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=39.1397849462366, Blast_Score=326, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=40.301724137931, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=40.301724137931, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=39.0086206896552, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=39.0086206896552, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24583245, Length=453, Percent_Identity=34.4370860927152, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52799; Mature: 52668

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMLDLDTKNTAATRIEEDLLGERHVPAEAYYGIHTLRAIENFNISNVTISDVPEFVRG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MIYTKKAAALANKELGAIPSQVGEYIIKACDLILETGKCMDQFPSDVFQGGAGTSVNMNA
HHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCH
NEVIANVALELMGKEKGEYDIVNPNDHVNKSQSTNCAYPTGFRIAVYNSMMKLIEAIEYL
HHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGAFDLKDQEFRNILKMGRTQLQDAVPMTVGQEFHAWGVLLQEEVKNLHYTAQLLLEVNI
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
GATAIGTGLNAATGYQEAAVKHLSEVTGLPCVAAEDLIEATSDCGAYVMIHGALKRTAVK
CCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
MSKICNDLRLLSSGPRAGLNELNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
TFAAEAGQLQLNVMEPVIGQALFESIDILRNACVNLRDKCIDGITVNKEVCEAHVFNSIG
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
IVTYLNPFIGHHEGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSVENLMHPEYKAK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCC
RYD
CCC
>Mature Secondary Structure 
TQMLDLDTKNTAATRIEEDLLGERHVPAEAYYGIHTLRAIENFNISNVTISDVPEFVRG
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MIYTKKAAALANKELGAIPSQVGEYIIKACDLILETGKCMDQFPSDVFQGGAGTSVNMNA
HHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCH
NEVIANVALELMGKEKGEYDIVNPNDHVNKSQSTNCAYPTGFRIAVYNSMMKLIEAIEYL
HHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGAFDLKDQEFRNILKMGRTQLQDAVPMTVGQEFHAWGVLLQEEVKNLHYTAQLLLEVNI
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
GATAIGTGLNAATGYQEAAVKHLSEVTGLPCVAAEDLIEATSDCGAYVMIHGALKRTAVK
CCCHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
MSKICNDLRLLSSGPRAGLNELNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
TFAAEAGQLQLNVMEPVIGQALFESIDILRNACVNLRDKCIDGITVNKEVCEAHVFNSIG
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
IVTYLNPFIGHHEGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSVENLMHPEYKAK
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCC
RYD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]