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The map label for this gene is amiB [H]

Identifier: 59712933

GI number: 59712933

Start: 2613696

End: 2615426

Strand: Reverse

Name: amiB [H]

Synonym: VF_2326

Alternate gene names: 59712933

Gene position: 2615426-2613696 (Counterclockwise)

Preceding gene: 59712934

Following gene: 59712932

Centisome position: 90.26

GC content: 37.03

Gene sequence:

>1731_bases
GTGTTAATCGGTAAATATTTTCGAATTATTTGGTATACGCTATTTGTTGTTCTTTCAACAAGTAGCTTTTTTGTATCTGC
AAACACGTTAGAGGGAATACGAGTTTGGCCTTCGCCTGATGAAACTCGTATTGTTATTGACTTAAATTCAGAAGCAAATT
ACAGCTACTTTACATTAAGTAGTCCATACCGTTTGGTTGTTGATCTTAAGAATACGAATGTAAAAGCCAAGTTGCCTTTA
AATGTTAAAGACAGCAAGGTACTGACTAAGATAAGAAAGAGTTCACCACCATCAAAAAACACTACACGTTTGGTTTTTGA
ATTAAAGCACTCTGTTATGGCTGAGGTGTTTAAGTTAGCACCAACTCCAGGTGGGCAGTATGGGCACCGTCTTGTTATGG
ATTTACCTCATTCAAAAACAAGCAGTTCAACTCCAGCACCAAGTAAACCATCTAAACCCGTAATAAATTCAAGTAGTAAT
CATATTGTTGGTAATGGCGAAATTATTATTGCTATTGATGCTGGGCATGGTGGTGAAGATCCAGGCTCGATTGGACCAAC
AAGAAAGTATGAAAAACACGCTACATTAGCTATTTCAAAGAAAGTCGTTGCTCAAATTAATGCTCAACCAGGTATGCGAG
CGGTAATGACTCGTAGTGGTGATTATTTTGTTAATTTAAACAAGCGTACTGAGATTGCTAGGAAAAATAAGTCGCATTTA
TTAGTATCTATTCACGCTGATAGTTTCCGTTCACCACAGCCAAGAGGGGGTTCTGTTTTTGTATTGAATACTCGCAGAGC
GAATACTGAGATTGGTCGCTGGGTTGAAAAACATGAGAAACAATCTGAATTATTGGGTGGTGCTGGAGATGTTCTCGCTA
AAAATAGTAACGACAGAAATGTAAGCCAAACGTTATTAGATTTGCAATTTAGTCACTCTCAAAAAGAAGGCTATAAATTA
GCGAAGAATGTGTTATCTGAAATGGGAAAAGTGATTCGACTACATAAAAAGGAGCCAGTAAACGCAAGTTTGGCTGTATT
AAAATCACCAGATATCCCATCTATTTTGATTGAGACCGGCTTTATTTCTAATCCAACGGAAGAAAAGCAATTATTCCAGC
GAGCTCATCAAGATAAAATTGCACGTTCTATTTCAAAAGCAATCATTCGTTATCTTCATGATAATCCGCCGCAAGGTACG
CTACTTGCTGCACAAGGTGGTCAAACAAAACATAAAGTACGTTCAGGTGAGTCTTTGTCTTTAATTGCAAAGAAATACGG
AACGACGATGGCGAAAATTAAAAGTGAAAATAATCTTAAAAGCACGAGCTTAAAGATTGGACAAGTTTTAGTTATCCCAA
GTAATAAATCTGTGTATGTTGCACCGAAGAATACGGCTTCTTCTGTGAATACTTCATCAAGTCAGGCTCGTACTATTATT
ACTCATAAAGTTCGTTCTGGTGAGTATTTAGGAAAGATTGCATCGAAATATAAAGTAACAACAAGCAGTATCCGATCATT
GAATAAATTGAAGTCTGATACCTTGTTTGTTGGACAAAAATTGAAAGTGAGTATTGTTGCTCCTGTTAAGAAACACACGG
TGAAACGTGGTGAATTCTTAGGTAAAATTGCAAGTAAATATGGCGTATCTGTTTCCAGCTTAAGAAAAGCAAACAAGTTA
CGTTCAGATGAGCTTGCTATAGGGCAGGTGCTCATTATTCCAACCAGTTAA

Upstream 100 bases:

>100_bases
CAGATCTTGATATTGATATTCGCTACGATGGTGAAGCTCGTCATGTTGTGATCACAGGTAATAATGAGTATGGATGTAGT
TTAGTACAGCGGTTGGATTT

Downstream 100 bases:

>100_bases
GGAACAAGGTTATGCCAATTCAAATCTTACCAGCACGATTAGCCAACCAAATTGCGGCGGGTGAGGTTGTGGAGCGTCCG
GCTTCTGTTGTTAAAGAATT

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MLIGKYFRIIWYTLFVVLSTSSFFVSANTLEGIRVWPSPDETRIVIDLNSEANYSYFTLSSPYRLVVDLKNTNVKAKLPL
NVKDSKVLTKIRKSSPPSKNTTRLVFELKHSVMAEVFKLAPTPGGQYGHRLVMDLPHSKTSSSTPAPSKPSKPVINSSSN
HIVGNGEIIIAIDAGHGGEDPGSIGPTRKYEKHATLAISKKVVAQINAQPGMRAVMTRSGDYFVNLNKRTEIARKNKSHL
LVSIHADSFRSPQPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRNVSQTLLDLQFSHSQKEGYKL
AKNVLSEMGKVIRLHKKEPVNASLAVLKSPDIPSILIETGFISNPTEEKQLFQRAHQDKIARSISKAIIRYLHDNPPQGT
LLAAQGGQTKHKVRSGESLSLIAKKYGTTMAKIKSENNLKSTSLKIGQVLVIPSNKSVYVAPKNTASSVNTSSSQARTII
THKVRSGEYLGKIASKYKVTTSSIRSLNKLKSDTLFVGQKLKVSIVAPVKKHTVKRGEFLGKIASKYGVSVSSLRKANKL
RSDELAIGQVLIIPTS

Sequences:

>Translated_576_residues
MLIGKYFRIIWYTLFVVLSTSSFFVSANTLEGIRVWPSPDETRIVIDLNSEANYSYFTLSSPYRLVVDLKNTNVKAKLPL
NVKDSKVLTKIRKSSPPSKNTTRLVFELKHSVMAEVFKLAPTPGGQYGHRLVMDLPHSKTSSSTPAPSKPSKPVINSSSN
HIVGNGEIIIAIDAGHGGEDPGSIGPTRKYEKHATLAISKKVVAQINAQPGMRAVMTRSGDYFVNLNKRTEIARKNKSHL
LVSIHADSFRSPQPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRNVSQTLLDLQFSHSQKEGYKL
AKNVLSEMGKVIRLHKKEPVNASLAVLKSPDIPSILIETGFISNPTEEKQLFQRAHQDKIARSISKAIIRYLHDNPPQGT
LLAAQGGQTKHKVRSGESLSLIAKKYGTTMAKIKSENNLKSTSLKIGQVLVIPSNKSVYVAPKNTASSVNTSSSQARTII
THKVRSGEYLGKIASKYKVTTSSIRSLNKLKSDTLFVGQKLKVSIVAPVKKHTVKRGEFLGKIASKYGVSVSSLRKANKL
RSDELAIGQVLIIPTS
>Mature_576_residues
MLIGKYFRIIWYTLFVVLSTSSFFVSANTLEGIRVWPSPDETRIVIDLNSEANYSYFTLSSPYRLVVDLKNTNVKAKLPL
NVKDSKVLTKIRKSSPPSKNTTRLVFELKHSVMAEVFKLAPTPGGQYGHRLVMDLPHSKTSSSTPAPSKPSKPVINSSSN
HIVGNGEIIIAIDAGHGGEDPGSIGPTRKYEKHATLAISKKVVAQINAQPGMRAVMTRSGDYFVNLNKRTEIARKNKSHL
LVSIHADSFRSPQPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRNVSQTLLDLQFSHSQKEGYKL
AKNVLSEMGKVIRLHKKEPVNASLAVLKSPDIPSILIETGFISNPTEEKQLFQRAHQDKIARSISKAIIRYLHDNPPQGT
LLAAQGGQTKHKVRSGESLSLIAKKYGTTMAKIKSENNLKSTSLKIGQVLVIPSNKSVYVAPKNTASSVNTSSSQARTII
THKVRSGEYLGKIASKYKVTTSSIRSLNKLKSDTLFVGQKLKVSIVAPVKKHTVKRGEFLGKIASKYGVSVSSLRKANKL
RSDELAIGQVLIIPTS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=411, Percent_Identity=38.4428223844282, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI87082163, Length=417, Percent_Identity=35.0119904076739, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788776, Length=230, Percent_Identity=38.2608695652174, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 63185; Mature: 63185

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGKYFRIIWYTLFVVLSTSSFFVSANTLEGIRVWPSPDETRIVIDLNSEANYSYFTLS
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCEEEEEEC
SPYRLVVDLKNTNVKAKLPLNVKDSKVLTKIRKSSPPSKNTTRLVFELKHSVMAEVFKLA
CCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHC
PTPGGQYGHRLVMDLPHSKTSSSTPAPSKPSKPVINSSSNHIVGNGEIIIAIDAGHGGED
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCC
PGSIGPTRKYEKHATLAISKKVVAQINAQPGMRAVMTRSGDYFVNLNKRTEIARKNKSHL
CCCCCCCCCHHCHHHHHHHHHHHHEECCCCCCEEEEECCCCEEEEECCHHHHHHCCCCEE
LVSIHADSFRSPQPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRN
EEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCH
VSQTLLDLQFSHSQKEGYKLAKNVLSEMGKVIRLHKKEPVNASLAVLKSPDIPSILIETG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCEEEEECC
FISNPTEEKQLFQRAHQDKIARSISKAIIRYLHDNPPQGTLLAAQGGQTKHKVRSGESLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCHH
LIAKKYGTTMAKIKSENNLKSTSLKIGQVLVIPSNKSVYVAPKNTASSVNTSSSQARTII
HHHHHHCCCEEEECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCHHEEEE
THKVRSGEYLGKIASKYKVTTSSIRSLNKLKSDTLFVGQKLKVSIVAPVKKHTVKRGEFL
EEECCCCHHHHHHHHHHEECHHHHHHHHHHCCCEEEECCEEEEEEECCHHHHHHHHHHHH
GKIASKYGVSVSSLRKANKLRSDELAIGQVLIIPTS
HHHHHHHCCCHHHHHHHHHCCCCCEEECEEEEEECC
>Mature Secondary Structure
MLIGKYFRIIWYTLFVVLSTSSFFVSANTLEGIRVWPSPDETRIVIDLNSEANYSYFTLS
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCEEEEEEC
SPYRLVVDLKNTNVKAKLPLNVKDSKVLTKIRKSSPPSKNTTRLVFELKHSVMAEVFKLA
CCEEEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHC
PTPGGQYGHRLVMDLPHSKTSSSTPAPSKPSKPVINSSSNHIVGNGEIIIAIDAGHGGED
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCC
PGSIGPTRKYEKHATLAISKKVVAQINAQPGMRAVMTRSGDYFVNLNKRTEIARKNKSHL
CCCCCCCCCHHCHHHHHHHHHHHHEECCCCCCEEEEECCCCEEEEECCHHHHHHCCCCEE
LVSIHADSFRSPQPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRN
EEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCH
VSQTLLDLQFSHSQKEGYKLAKNVLSEMGKVIRLHKKEPVNASLAVLKSPDIPSILIETG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCEEEEECC
FISNPTEEKQLFQRAHQDKIARSISKAIIRYLHDNPPQGTLLAAQGGQTKHKVRSGESLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCHH
LIAKKYGTTMAKIKSENNLKSTSLKIGQVLVIPSNKSVYVAPKNTASSVNTSSSQARTII
HHHHHHCCCEEEECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCHHEEEE
THKVRSGEYLGKIASKYKVTTSSIRSLNKLKSDTLFVGQKLKVSIVAPVKKHTVKRGEFL
EEECCCCHHHHHHHHHHEECHHHHHHHHHHCCCEEEECCEEEEEEECCHHHHHHHHHHHH
GKIASKYGVSVSSLRKANKLRSDELAIGQVLIIPTS
HHHHHHHCCCHHHHHHHHHCCCCCEEECEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]