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The map label for this gene is metB [H]

Identifier: 59712874

GI number: 59712874

Start: 2546806

End: 2547972

Strand: Reverse

Name: metB [H]

Synonym: VF_2267

Alternate gene names: 59712874

Gene position: 2547972-2546806 (Counterclockwise)

Preceding gene: 59712879

Following gene: 59712873

Centisome position: 87.94

GC content: 40.79

Gene sequence:

>1167_bases
ATGAGCGAAAGAAAACCTGCAACTATTGCTGTTCGTACCGGCATTGAAACAGACTCACAACATAACGCTGTTGTGCCACC
TATCTACTTATCAACTAACTATAGTTTCCCAGAGTTTGGGGATGTACCTAAGTATGATTATGCTCGTTCTGGAAATCCAA
CTCGAACTCAATTAGAAACCACACTTTCAGATTTAGAAGGTGGGGCTGGTGCAGTGGTCACTAATTGTGGAACATCCGCT
CTTAATTTAATGGTATCTGCATTACTTGGTCCTGACGATTTAATTGTTGCTCCTCATGATTGTTATGGAGGAACTTATCG
ATTGTTTAATACTCGAGCAAATAAAGGAGACTTCCAAACTTTATTTGTAGACCAATCTAATACTGAGGCTTTAGATGCTG
CAATTGCTAAAAAGCCAAAATTAATCCTACTTGAAACCCCTTCAAATCCATTAGTACGTGTGGTGGATATTGCTGAAATT
TGCCAAAAAGCGGCTCAAGTTGGTGCGTTAGTCGCAGTAGATAACACTTTCCTTTCTCCTGTATTACAGCAACCTCTTTC
TTTAGGTGCTGATTTTGTTATCCACTCTACAACCAAATACATTAATGGGCATTCTGATGTTGTTGGTGGAGTATTGATCG
CTAAAACAAAAGCGCATGCGGATGATCTTGCATGGTGGGGAAATTGCATTGGAGCGACGGGTACCGCATTCGATAGTTAT
TTAACACTGCGTGGTATTCGAACTCTTGGTGCTCGTATGCGTGTGCATGAAGAAAATGCAATAGAAGTACTCAAGTGCCT
TCAAGAGCAGCCATTAGTCGGTACGATTTATCACCCGAGTTTACCTGAGCATCCAGGTCATGAAATTGCAAAACGTCAAC
AAGTAGGCTTTGGTGCCATGCTCAGTTTTGAATTAAATGGCTCACTTGAGCAATTGAAAGTATTCGTTAAAGAATTGAAA
TATTTCTCTTTAGCTGAATCTCTAGGTGGTGTTGAAAGTTTGATTGCGCACCCAGGAACCATGACACATAGAGCGATGTC
TGATGAAGCTCAAGAAGAAGCGGGATTAGCTCCAACGTTATTGAGATTATCTGTTGGATTAGAAGATAGCCGAGATCTTA
TTGCTGATCTTCAACAAGCATTTGCGATTGCTGCAGAGGTGAAATAA

Upstream 100 bases:

>100_bases
AAATTTAAGACGTGAAGATAAAGACATTCCCTTGTAAGCCTGTTTAGATAGTTTATATCGTTGGTATTACATTACTGCAC
CGCAGTTTGGAAGGGAAATT

Downstream 100 bases:

>100_bases
TGACAATACCTTGTTCACGTCAATTACATAAATTTGGTGGTAGCAGCTTGGCTGATCCAGAGTGCTATCGCCGAGTTGCC
ACCATCTTAAAAGAATACTC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MSERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPKYDYARSGNPTRTQLETTLSDLEGGAGAVVTNCGTSA
LNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQTLFVDQSNTEALDAAIAKKPKLILLETPSNPLVRVVDIAEI
CQKAAQVGALVAVDNTFLSPVLQQPLSLGADFVIHSTTKYINGHSDVVGGVLIAKTKAHADDLAWWGNCIGATGTAFDSY
LTLRGIRTLGARMRVHEENAIEVLKCLQEQPLVGTIYHPSLPEHPGHEIAKRQQVGFGAMLSFELNGSLEQLKVFVKELK
YFSLAESLGGVESLIAHPGTMTHRAMSDEAQEEAGLAPTLLRLSVGLEDSRDLIADLQQAFAIAAEVK

Sequences:

>Translated_388_residues
MSERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPKYDYARSGNPTRTQLETTLSDLEGGAGAVVTNCGTSA
LNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQTLFVDQSNTEALDAAIAKKPKLILLETPSNPLVRVVDIAEI
CQKAAQVGALVAVDNTFLSPVLQQPLSLGADFVIHSTTKYINGHSDVVGGVLIAKTKAHADDLAWWGNCIGATGTAFDSY
LTLRGIRTLGARMRVHEENAIEVLKCLQEQPLVGTIYHPSLPEHPGHEIAKRQQVGFGAMLSFELNGSLEQLKVFVKELK
YFSLAESLGGVESLIAHPGTMTHRAMSDEAQEEAGLAPTLLRLSVGLEDSRDLIADLQQAFAIAAEVK
>Mature_387_residues
SERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPKYDYARSGNPTRTQLETTLSDLEGGAGAVVTNCGTSAL
NLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQTLFVDQSNTEALDAAIAKKPKLILLETPSNPLVRVVDIAEIC
QKAAQVGALVAVDNTFLSPVLQQPLSLGADFVIHSTTKYINGHSDVVGGVLIAKTKAHADDLAWWGNCIGATGTAFDSYL
TLRGIRTLGARMRVHEENAIEVLKCLQEQPLVGTIYHPSLPEHPGHEIAKRQQVGFGAMLSFELNGSLEQLKVFVKELKY
FSLAESLGGVESLIAHPGTMTHRAMSDEAQEEAGLAPTLLRLSVGLEDSRDLIADLQQAFAIAAEVK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=40.6593406593407, Blast_Score=268, Evalue=6e-72,
Organism=Homo sapiens, GI299473759, Length=364, Percent_Identity=37.9120879120879, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=35.4570637119114, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=60.3674540682415, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1789383, Length=340, Percent_Identity=32.6470588235294, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=43.1937172774869, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17560056, Length=381, Percent_Identity=42.5196850393701, Blast_Score=277, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=34.0482573726542, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=37.8865979381443, Blast_Score=239, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6321254, Length=413, Percent_Identity=35.1089588377724, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=32.1678321678322, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=32.2335025380711, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=327, Percent_Identity=27.5229357798165, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322970, Length=263, Percent_Identity=26.2357414448669, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922554, Length=383, Percent_Identity=40.7310704960835, Blast_Score=288, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41769; Mature: 41638

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPKYDYARSGNPTRTQLET
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TLSDLEGGAGAVVTNCGTSALNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQT
HHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCCCCEEE
LFVDQSNTEALDAAIAKKPKLILLETPSNPLVRVVDIAEICQKAAQVGALVAVDNTFLSP
EEEECCCCHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCEEEEECHHHHHH
VLQQPLSLGADFVIHSTTKYINGHSDVVGGVLIAKTKAHADDLAWWGNCIGATGTAFDSY
HHHHHHHCCCCEEEECCHHHCCCCCHHHCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHH
LTLRGIRTLGARMRVHEENAIEVLKCLQEQPLVGTIYHPSLPEHPGHEIAKRQQVGFGAM
HHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCEE
LSFELNGSLEQLKVFVKELKYFSLAESLGGVESLIAHPGTMTHRAMSDEAQEEAGLAPTL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCEE
LRLSVGLEDSRDLIADLQQAFAIAAEVK
HHEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SERKPATIAVRTGIETDSQHNAVVPPIYLSTNYSFPEFGDVPKYDYARSGNPTRTQLET
CCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHH
TLSDLEGGAGAVVTNCGTSALNLMVSALLGPDDLIVAPHDCYGGTYRLFNTRANKGDFQT
HHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCCCCEEE
LFVDQSNTEALDAAIAKKPKLILLETPSNPLVRVVDIAEICQKAAQVGALVAVDNTFLSP
EEEECCCCHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCEEEEECHHHHHH
VLQQPLSLGADFVIHSTTKYINGHSDVVGGVLIAKTKAHADDLAWWGNCIGATGTAFDSY
HHHHHHHCCCCEEEECCHHHCCCCCHHHCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHH
LTLRGIRTLGARMRVHEENAIEVLKCLQEQPLVGTIYHPSLPEHPGHEIAKRQQVGFGAM
HHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCEE
LSFELNGSLEQLKVFVKELKYFSLAESLGGVESLIAHPGTMTHRAMSDEAQEEAGLAPTL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCEE
LRLSVGLEDSRDLIADLQQAFAIAAEVK
HHEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]