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The map label for this gene is metL [H]

Identifier: 59712873

GI number: 59712873

Start: 2544389

End: 2546806

Strand: Reverse

Name: metL [H]

Synonym: VF_2266

Alternate gene names: 59712873

Gene position: 2546806-2544389 (Counterclockwise)

Preceding gene: 59712874

Following gene: 59712872

Centisome position: 87.9

GC content: 41.56

Gene sequence:

>2418_bases
ATGACAATACCTTGTTCACGTCAATTACATAAATTTGGTGGTAGCAGCTTGGCTGATCCAGAGTGCTATCGCCGAGTTGC
CACCATCTTAAAAGAATACTCAAATGAACATGATCTGATTGTTGTTTCTGCTGCAGGTAATACAACAAACCGTATTTTAG
AATGGATCGATGACCTATCTAAAGATGGGCGCTTAGCTCATGAAACATTAGAGTCTTTACGTACATACCAACAAGGGTTG
ATAACCGAATTACTGTCTGCTGAGAATGCAGAACCTTTATTGACCGTTTTATACCAAGAATTGATTGCGATAGGGCAATT
ACATGCGCCGTTAACAGAGGTGCAGCAAGCTGATGTATTAGGTCATGGTGAAGTGTGGTCTTCAAGGTTGCTTTCTGCGT
ATTTAAATCAAATCGATTTACAAGCAGCTCCTCTTGATTCTCGAACGTTTCTAAGGGCAGAGCGAGCAGCACAACCTGAA
GTGGATCGTGTGCAGTCTTGGCCTTTGTTTAAAGAGCAGGTCGCTCAGCATCCGCATGGACGAATTGTGATTACTGGTTT
TATGGCCCGTAATCAAAGAGGAGAAACCGTACTTCTTGGTCGAAATGGTTCTGATTATTCGGCAACCATGATTGGTGTCC
TTGGAGAGGTGTCATGCGTTACTATCTGGAGTGATGTTGCTGGCGTGTTCAGTGCGGATCCTAGAAAAGTGGAGGATGCG
TGCTTACTGCCATTATTAAGATTAGATGAAGCGAGTGAATTAGCTCGTTTAGCCGCTCCTGTATTGCACAGTAGAACATT
ACAACCTGTAGCTCAAAGTGCATTAGATTTGAGTTTACGTTGCAGTTATCAACCTGAATCTGGTTCAACCCGAATCGAGC
GTGTATTGGCTTCTGGGCGAGGTGCAAAAATTGTTACGTCATTAGAAGATGTTTATTTGATTCAGCTTGGCTTTGGTCGT
GGACAATATTTTGAACGCGCTCAACAAGAGGTTCTTTCTTTACTTACTCGTTCTCAACTGCAACCACTGGCCTTAGAGCC
TCAAGCTGATCAGTTTACATTATTGCTCGCTTATACTTCTGAAGTCGTGAATGAAGCGTTAGAAGTGCTACAAAACTCAG
CGGTACATGCTGAAATTAAGCTAAAAGAAGGCTATTCAATGGTTGCTGCTGTTGGCGCGGGGGTTACAGCGAATGCTATT
CACAGTCATGGTTTTTATCATCAATTGAAAAATTGCCCTCTTGAATTTGTGTACGCATCAGAATCGGGCTTAAGTCTTGT
CGCTATTTTACGTAAAGTTGATACCTCATCGATCATTAATGCCATTCATAACAGCTTATTCCAAGCGCAAAAACGAGTGG
GATTGATCCTGTGTGGACATGGAAATATTGGTTCTCAATGGTTATCACTATTTACTCAACAAAAACGTCAACTTGAAAAG
CGTCATGGAATGCAGTTTTCACTCATTGGTGTTGTCGATAGAGAACGTTGTTGGCTTGATTTTGATGGGTTAAACAGTAC
TGAGGTGGTTGCTAAGTTTGATGATGAAGCAACAAACTATACGGATTTTGATTGGATTGAAAAAGTCGGTGCCGTTCAAG
GTTATGATGATGTTGTTGTTTTAGATGTAACGGCAAGTGCTGATCTTGCTAATCGATATTCAAAAATTGCAGAACAAGGA
ATGCATCTGATCTCAGCGAATAAAGTTGCTGGCTCAGCGCCAAGTGCTGATTATCAAGCGGTAATTGATGCTTTTGCTAA
AACATCGCGTCACTGGTTATATAATGCAACCGTTGGAGCAGGCTTACCTATTAATCATACCGTAAGAGATCTTCGTGAAA
GTGGTGATGAAATTATCGCATTATCGGGCATTTTCTCTGGTACGTTATCGTGGCTGTTTCAGCAATATAATGGCGATGTT
CCTTTTACTGAATTAGTAGATTTGGCTTGGCAACAAGGGTTGACTGAACCGGATCCAAGACACGATCTTGATGGTTCAGA
TGTCATGCGCAAATTGGTGATCCTTGCTCGTGAAGCCGGGCTTCATATTGAGCCAAATCAGGTAAAAGTTGAATCACTTG
TTCCTAGTGAATTATTAGAAACCTGTCTTGATGATTTTCTGTCTCAAGGTGAGGTGATTAATGAATTATTGCTTGAACGA
TTAACAAAAGCTCAGAAAGAACAGAAAGTATTACGCTATGTGGCTCGTTTAAATAAAGACGGTAGCGCAACGGTTGGAAT
CGAGGCATTACCTGAATATCACGCATTAGCCAATCTACTACCATGCGATAATATTTTTGCCATAGAGAGCAAATGGTATA
AAGATAATCCACTGGTTATTCGAGGCCCAGGAGCTGGACGAGAGGTGACTGCAGGTGCGATTCAATCTGATTTAAATCGT
ATTGCTTCGCTGTTTTAA

Upstream 100 bases:

>100_bases
TAGCTCCAACGTTATTGAGATTATCTGTTGGATTAGAAGATAGCCGAGATCTTATTGCTGATCTTCAACAAGCATTTGCG
ATTGCTGCAGAGGTGAAATA

Downstream 100 bases:

>100_bases
CATCATTTAACCTTATGCATATTTATTAAGCCATACACCTTGTATGGCTTTTTTGTACCTATTGTCTTGAAATACGCATA
TTTTCAATATTTAGATTAAT

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MTIPCSRQLHKFGGSSLADPECYRRVATILKEYSNEHDLIVVSAAGNTTNRILEWIDDLSKDGRLAHETLESLRTYQQGL
ITELLSAENAEPLLTVLYQELIAIGQLHAPLTEVQQADVLGHGEVWSSRLLSAYLNQIDLQAAPLDSRTFLRAERAAQPE
VDRVQSWPLFKEQVAQHPHGRIVITGFMARNQRGETVLLGRNGSDYSATMIGVLGEVSCVTIWSDVAGVFSADPRKVEDA
CLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGRGAKIVTSLEDVYLIQLGFGR
GQYFERAQQEVLSLLTRSQLQPLALEPQADQFTLLLAYTSEVVNEALEVLQNSAVHAEIKLKEGYSMVAAVGAGVTANAI
HSHGFYHQLKNCPLEFVYASESGLSLVAILRKVDTSSIINAIHNSLFQAQKRVGLILCGHGNIGSQWLSLFTQQKRQLEK
RHGMQFSLIGVVDRERCWLDFDGLNSTEVVAKFDDEATNYTDFDWIEKVGAVQGYDDVVVLDVTASADLANRYSKIAEQG
MHLISANKVAGSAPSADYQAVIDAFAKTSRHWLYNATVGAGLPINHTVRDLRESGDEIIALSGIFSGTLSWLFQQYNGDV
PFTELVDLAWQQGLTEPDPRHDLDGSDVMRKLVILAREAGLHIEPNQVKVESLVPSELLETCLDDFLSQGEVINELLLER
LTKAQKEQKVLRYVARLNKDGSATVGIEALPEYHALANLLPCDNIFAIESKWYKDNPLVIRGPGAGREVTAGAIQSDLNR
IASLF

Sequences:

>Translated_805_residues
MTIPCSRQLHKFGGSSLADPECYRRVATILKEYSNEHDLIVVSAAGNTTNRILEWIDDLSKDGRLAHETLESLRTYQQGL
ITELLSAENAEPLLTVLYQELIAIGQLHAPLTEVQQADVLGHGEVWSSRLLSAYLNQIDLQAAPLDSRTFLRAERAAQPE
VDRVQSWPLFKEQVAQHPHGRIVITGFMARNQRGETVLLGRNGSDYSATMIGVLGEVSCVTIWSDVAGVFSADPRKVEDA
CLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGRGAKIVTSLEDVYLIQLGFGR
GQYFERAQQEVLSLLTRSQLQPLALEPQADQFTLLLAYTSEVVNEALEVLQNSAVHAEIKLKEGYSMVAAVGAGVTANAI
HSHGFYHQLKNCPLEFVYASESGLSLVAILRKVDTSSIINAIHNSLFQAQKRVGLILCGHGNIGSQWLSLFTQQKRQLEK
RHGMQFSLIGVVDRERCWLDFDGLNSTEVVAKFDDEATNYTDFDWIEKVGAVQGYDDVVVLDVTASADLANRYSKIAEQG
MHLISANKVAGSAPSADYQAVIDAFAKTSRHWLYNATVGAGLPINHTVRDLRESGDEIIALSGIFSGTLSWLFQQYNGDV
PFTELVDLAWQQGLTEPDPRHDLDGSDVMRKLVILAREAGLHIEPNQVKVESLVPSELLETCLDDFLSQGEVINELLLER
LTKAQKEQKVLRYVARLNKDGSATVGIEALPEYHALANLLPCDNIFAIESKWYKDNPLVIRGPGAGREVTAGAIQSDLNR
IASLF
>Mature_804_residues
TIPCSRQLHKFGGSSLADPECYRRVATILKEYSNEHDLIVVSAAGNTTNRILEWIDDLSKDGRLAHETLESLRTYQQGLI
TELLSAENAEPLLTVLYQELIAIGQLHAPLTEVQQADVLGHGEVWSSRLLSAYLNQIDLQAAPLDSRTFLRAERAAQPEV
DRVQSWPLFKEQVAQHPHGRIVITGFMARNQRGETVLLGRNGSDYSATMIGVLGEVSCVTIWSDVAGVFSADPRKVEDAC
LLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGRGAKIVTSLEDVYLIQLGFGRG
QYFERAQQEVLSLLTRSQLQPLALEPQADQFTLLLAYTSEVVNEALEVLQNSAVHAEIKLKEGYSMVAAVGAGVTANAIH
SHGFYHQLKNCPLEFVYASESGLSLVAILRKVDTSSIINAIHNSLFQAQKRVGLILCGHGNIGSQWLSLFTQQKRQLEKR
HGMQFSLIGVVDRERCWLDFDGLNSTEVVAKFDDEATNYTDFDWIEKVGAVQGYDDVVVLDVTASADLANRYSKIAEQGM
HLISANKVAGSAPSADYQAVIDAFAKTSRHWLYNATVGAGLPINHTVRDLRESGDEIIALSGIFSGTLSWLFQQYNGDVP
FTELVDLAWQQGLTEPDPRHDLDGSDVMRKLVILAREAGLHIEPNQVKVESLVPSELLETCLDDFLSQGEVINELLLERL
TKAQKEQKVLRYVARLNKDGSATVGIEALPEYHALANLLPCDNIFAIESKWYKDNPLVIRGPGAGREVTAGAIQSDLNRI
ASLF

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=804, Percent_Identity=59.0796019900497, Blast_Score=943, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=833, Percent_Identity=28.3313325330132, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1790455, Length=456, Percent_Identity=25.219298245614, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322599, Length=345, Percent_Identity=33.0434782608696, Blast_Score=164, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6320893, Length=382, Percent_Identity=23.0366492146597, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88741; Mature: 88609

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPCSRQLHKFGGSSLADPECYRRVATILKEYSNEHDLIVVSAAGNTTNRILEWIDDLS
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHC
KDGRLAHETLESLRTYQQGLITELLSAENAEPLLTVLYQELIAIGQLHAPLTEVQQADVL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
GHGEVWSSRLLSAYLNQIDLQAAPLDSRTFLRAERAAQPEVDRVQSWPLFKEQVAQHPHG
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHCCCCC
RIVITGFMARNQRGETVLLGRNGSDYSATMIGVLGEVSCVTIWSDVAGVFSADPRKVEDA
CEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCHHEEHHHHHHHHHCCCCCHHHHH
CLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHCCC
GAKIVTSLEDVYLIQLGFGRGQYFERAQQEVLSLLTRSQLQPLALEPQADQFTLLLAYTS
CCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEHHHHHH
EVVNEALEVLQNSAVHAEIKLKEGYSMVAAVGAGVTANAIHSHGFYHQLKNCPLEFVYAS
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEC
ESGLSLVAILRKVDTSSIINAIHNSLFQAQKRVGLILCGHGNIGSQWLSLFTQQKRQLEK
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHH
RHGMQFSLIGVVDRERCWLDFDGLNSTEVVAKFDDEATNYTDFDWIEKVGAVQGYDDVVV
HCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEE
LDVTASADLANRYSKIAEQGMHLISANKVAGSAPSADYQAVIDAFAKTSRHWLYNATVGA
EEEECCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
GLPINHTVRDLRESGDEIIALSGIFSGTLSWLFQQYNGDVPFTELVDLAWQQGLTEPDPR
CCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
HDLDGSDVMRKLVILAREAGLHIEPNQVKVESLVPSELLETCLDDFLSQGEVINELLLER
CCCCHHHHHHHHHHHHHHCCCEECCCCEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LTKAQKEQKVLRYVARLNKDGSATVGIEALPEYHALANLLPCDNIFAIESKWYKDNPLVI
HHHHHHHHHHHHHHHHHCCCCCCEECHHHCHHHHHHHHHCCCCCEEEECCCEECCCCEEE
RGPGAGREVTAGAIQSDLNRIASLF
ECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIPCSRQLHKFGGSSLADPECYRRVATILKEYSNEHDLIVVSAAGNTTNRILEWIDDLS
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHC
KDGRLAHETLESLRTYQQGLITELLSAENAEPLLTVLYQELIAIGQLHAPLTEVQQADVL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
GHGEVWSSRLLSAYLNQIDLQAAPLDSRTFLRAERAAQPEVDRVQSWPLFKEQVAQHPHG
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHCCCCC
RIVITGFMARNQRGETVLLGRNGSDYSATMIGVLGEVSCVTIWSDVAGVFSADPRKVEDA
CEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCHHEEHHHHHHHHHCCCCCHHHHH
CLLPLLRLDEASELARLAAPVLHSRTLQPVAQSALDLSLRCSYQPESGSTRIERVLASGR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHCCC
GAKIVTSLEDVYLIQLGFGRGQYFERAQQEVLSLLTRSQLQPLALEPQADQFTLLLAYTS
CCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEHHHHHH
EVVNEALEVLQNSAVHAEIKLKEGYSMVAAVGAGVTANAIHSHGFYHQLKNCPLEFVYAS
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEC
ESGLSLVAILRKVDTSSIINAIHNSLFQAQKRVGLILCGHGNIGSQWLSLFTQQKRQLEK
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHH
RHGMQFSLIGVVDRERCWLDFDGLNSTEVVAKFDDEATNYTDFDWIEKVGAVQGYDDVVV
HCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEE
LDVTASADLANRYSKIAEQGMHLISANKVAGSAPSADYQAVIDAFAKTSRHWLYNATVGA
EEEECCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
GLPINHTVRDLRESGDEIIALSGIFSGTLSWLFQQYNGDVPFTELVDLAWQQGLTEPDPR
CCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
HDLDGSDVMRKLVILAREAGLHIEPNQVKVESLVPSELLETCLDDFLSQGEVINELLLER
CCCCHHHHHHHHHHHHHHCCCEECCCCEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LTKAQKEQKVLRYVARLNKDGSATVGIEALPEYHALANLLPCDNIFAIESKWYKDNPLVI
HHHHHHHHHHHHHHHHHCCCCCCEECHHHCHHHHHHHHHCCCCCEEEECCCEECCCCEEE
RGPGAGREVTAGAIQSDLNRIASLF
ECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]