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The map label for this gene is maeB [H]

Identifier: 59712879

GI number: 59712879

Start: 2553512

End: 2554759

Strand: Reverse

Name: maeB [H]

Synonym: VF_2272

Alternate gene names: 59712879

Gene position: 2554759-2553512 (Counterclockwise)

Preceding gene: 59712880

Following gene: 59712874

Centisome position: 88.17

GC content: 41.11

Gene sequence:

>1248_bases
ATGTCTGAAGAATTTCGCAAGCAAGCTCTAGATTATCACGCTTACCCAACTCCAGGTAAAATTGAAGTTGCATTGACTAA
ACCTGCTGACACAGTAGAAGACCTTGCTTTGGCTTACAGCCCTGGTGTGGCTGAGCCTGTACGTGAAATTGCAAAGAACC
CTGATGATGTTTATAAATACACATCAAAAGGCAATATGGTTGCGGTTATTTCAAATGGTACGGCAATTCTTGGTTTAGGT
AACTTAGGTCCTCTTGCATCAAAACCAGTAATGGAAGGTAAGTCGCTTCTTTTTAAACGTTTTGCAGGCTTAGACTCTAT
TGATATCGAAGTAAAACACCGCACGATTGATGAGTTTGTTGATACAGTTGCTAATATTGCAGATACATTTGGTGGTATTA
ACTTAGAAGATATTAAAGCACCTGATTGTTTTGAGATTGAACGTCGCCTTATTGAAATTTGTGATGTGCCAGTATTCCAT
GATGATCAACATGGTACTGCAATCGTAACTGCTGCTGGTATGTTAAATGCGATTGAGCTGCAAGGTAAGAAGTTAGAAGA
TGCGATCATTGTATGTTTAGGTGCTGGTGCAGCTGCCGTTGCATGTATGGAGCTGCTTATTAAGTGTGGCGCAATGCGTG
AGAAGATCTACATGCTGGATCGTAAAGGTGTGATTCATACTCGTCGTGACGATATTAATGAGTACAAACAACGTTTTGCA
AACAATACCGATAAGCGCACACTGCAAGATGTTATCGAAGGTGCCGATTTATTCTTGGGTGTATCTGGTCCAAATTTATT
AGATGCAGAAGATCTGAAGCTAATGGCTGATAAACCAGTTGTGTTTGCATGTTCAAATCCAGATCCTGAAATTAAACCTG
AATTGGCTCATGAAGTTCGTTCTGACTTAATCATGGGTACAGGCCGTTCTGATTATCCAAACCAAGTAAATAATGTGCTT
TGTTTCCCATTTATCTTCCGTGGTGCGCTAGATGTGCGTGCAAGTGAAATTAATGATGAAATGAAGCTAGCTGCTGTTGA
GGCTATTCGTCAATTAGCTAAAGAAGAAGTACCCGTTGAGGTATTAAAAGCCGCTGGCGTTGAATCCTTGACATTTGGCT
CTGACTATATCATTCCAAAACCAATGGATCCTCGTCTACTTCCACGAGTTGCTAAAGCGGTAGCACAAGCTGCTGTTGAC
TCCGGTGTCGCTCGTATAGAAATGCCTAAAGGATACATGGAAGCGTAA

Upstream 100 bases:

>100_bases
TGTTGGTACGATCTGTAAGCAAAGGCTATGAAGTTTGAGCTAGATCAACTAATATAGAGGTTTACTATTTTGACGTTACC
TTTTAAATCAGGAAACCATC

Downstream 100 bases:

>100_bases
TTCGCTTTATCTTTTATGTAGAAAAGGCTTCTAACTAATTAATTAAGTTAGAAGCTTTTTTTTATTTAGGAAAGAAATTA
TTTTTGACTTTCTAAAGCTT

Product: fused malic enzyme oxidoreductase/ phosphotransacetylase

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSEEFRKQALDYHAYPTPGKIEVALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKYTSKGNMVAVISNGTAILGLG
NLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIEICDVPVFH
DDQHGTAIVTAAGMLNAIELQGKKLEDAIIVCLGAGAAAVACMELLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
NNTDKRTLQDVIEGADLFLGVSGPNLLDAEDLKLMADKPVVFACSNPDPEIKPELAHEVRSDLIMGTGRSDYPNQVNNVL
CFPFIFRGALDVRASEINDEMKLAAVEAIRQLAKEEVPVEVLKAAGVESLTFGSDYIIPKPMDPRLLPRVAKAVAQAAVD
SGVARIEMPKGYMEA

Sequences:

>Translated_415_residues
MSEEFRKQALDYHAYPTPGKIEVALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKYTSKGNMVAVISNGTAILGLG
NLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIEICDVPVFH
DDQHGTAIVTAAGMLNAIELQGKKLEDAIIVCLGAGAAAVACMELLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
NNTDKRTLQDVIEGADLFLGVSGPNLLDAEDLKLMADKPVVFACSNPDPEIKPELAHEVRSDLIMGTGRSDYPNQVNNVL
CFPFIFRGALDVRASEINDEMKLAAVEAIRQLAKEEVPVEVLKAAGVESLTFGSDYIIPKPMDPRLLPRVAKAVAQAAVD
SGVARIEMPKGYMEA
>Mature_414_residues
SEEFRKQALDYHAYPTPGKIEVALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKYTSKGNMVAVISNGTAILGLGN
LGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFVDTVANIADTFGGINLEDIKAPDCFEIERRLIEICDVPVFHD
DQHGTAIVTAAGMLNAIELQGKKLEDAIIVCLGAGAAAVACMELLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFAN
NTDKRTLQDVIEGADLFLGVSGPNLLDAEDLKLMADKPVVFACSNPDPEIKPELAHEVRSDLIMGTGRSDYPNQVNNVLC
FPFIFRGALDVRASEINDEMKLAAVEAIRQLAKEEVPVEVLKAAGVESLTFGSDYIIPKPMDPRLLPRVAKAVAQAAVDS
GVARIEMPKGYMEA

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=355, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI62420880, Length=355, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI239049447, Length=355, Percent_Identity=28.169014084507, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4505143, Length=360, Percent_Identity=25.8333333333333, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI4505145, Length=354, Percent_Identity=27.9661016949153, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=59.1133004926108, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87081919, Length=503, Percent_Identity=23.8568588469185, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17537199, Length=415, Percent_Identity=25.3012048192771, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322823, Length=294, Percent_Identity=26.530612244898, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI78707242, Length=348, Percent_Identity=27.0114942528736, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707238, Length=348, Percent_Identity=27.0114942528736, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707240, Length=348, Percent_Identity=27.0114942528736, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281362674, Length=401, Percent_Identity=25.6857855361596, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281362672, Length=401, Percent_Identity=25.6857855361596, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356279, Length=401, Percent_Identity=25.6857855361596, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646388, Length=349, Percent_Identity=26.9340974212034, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=27.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363503, Length=366, Percent_Identity=24.5901639344262, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI281363505, Length=366, Percent_Identity=24.5901639344262, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78707236, Length=366, Percent_Identity=24.5901639344262, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78707232, Length=366, Percent_Identity=24.5901639344262, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 45273; Mature: 45141

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEFRKQALDYHAYPTPGKIEVALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKY
CCHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHEE
TSKGNMVAVISNGTAILGLGNLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFV
CCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
DTVANIADTFGGINLEDIKAPDCFEIERRLIEICDVPVFHDDQHGTAIVTAAGMLNAIEL
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEHHHHHHHHHH
QGKKLEDAIIVCLGAGAAAVACMELLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHC
NNTDKRTLQDVIEGADLFLGVSGPNLLDAEDLKLMADKPVVFACSNPDPEIKPELAHEVR
CCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHEEECCCEEEEECCCCCCCCHHHHHHHH
SDLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAAVEAIRQLAKEEVPVE
HHHEECCCCCCCCHHHHCEEEHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
VLKAAGVESLTFGSDYIIPKPMDPRLLPRVAKAVAQAAVDSGVARIEMPKGYMEA
HHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure 
SEEFRKQALDYHAYPTPGKIEVALTKPADTVEDLALAYSPGVAEPVREIAKNPDDVYKY
CHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHEE
TSKGNMVAVISNGTAILGLGNLGPLASKPVMEGKSLLFKRFAGLDSIDIEVKHRTIDEFV
CCCCCEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
DTVANIADTFGGINLEDIKAPDCFEIERRLIEICDVPVFHDDQHGTAIVTAAGMLNAIEL
HHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEHHHHHHHHHH
QGKKLEDAIIVCLGAGAAAVACMELLIKCGAMREKIYMLDRKGVIHTRRDDINEYKQRFA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHC
NNTDKRTLQDVIEGADLFLGVSGPNLLDAEDLKLMADKPVVFACSNPDPEIKPELAHEVR
CCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHEEECCCEEEEECCCCCCCCHHHHHHHH
SDLIMGTGRSDYPNQVNNVLCFPFIFRGALDVRASEINDEMKLAAVEAIRQLAKEEVPVE
HHHEECCCCCCCCHHHHCEEEHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
VLKAAGVESLTFGSDYIIPKPMDPRLLPRVAKAVAQAAVDSGVARIEMPKGYMEA
HHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]