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The map label for this gene is rfaE [H]

Identifier: 59712841

GI number: 59712841

Start: 2506450

End: 2507880

Strand: Reverse

Name: rfaE [H]

Synonym: VF_2234

Alternate gene names: 59712841

Gene position: 2507880-2506450 (Counterclockwise)

Preceding gene: 59712842

Following gene: 59712839

Centisome position: 86.55

GC content: 41.65

Gene sequence:

>1431_bases
ATGAAACCGACACTTCCAAATTATGATCAATCAAGCGTATTGATTGTTGGTGATGTGATGCTTGACCGTTACTGGGGTGG
CCCTACGAGTCGTATTTCACCAGAAGCACCAGTACCTGTTGTTAAAGTTGAAAAAATTGAAGAACGTCCAGGTGGTGCAG
CAAACGTAGCAATGAATATTGCTGCATTAGGTGGTGATGCACACTTAGTTGGATTGGTTGGTGAAGATGAGCCAGCACAA
GCATTAACAACAACATTAGAGTCATTAAAAGTACATTGTGACTTTGTTGCTCTTCCTGAATTTCCAACTATTACTAAATT
ACGAGTAATGAGTCGTGGTCAGCAACTTATTCGTTTGGATTTTGAAGATAGCTTCCATGATGTTGCTGCAGAACCGATTA
TTAGCCGTATGCAACAAGCACTATCAAGTGTAAAAGCCGTTGTTCTTTCTGATTATGCGAAAGGCGCTTTAGAGCACGTT
CAGTTGATGATTCAAGAAGCACGTAAAGTCAATGTACCTGTGTTTATTGATCCAAAAGGTGCCGATTTTGAGCGTTACCG
TGGTGCAACGTTATTAACGCCTAATATGCTTGAATTTGAAACGGTAGTTGGCAAAGTTAAAGATGAAGATGATCTAGTAG
CAAAAGGCCAACAGATTATCGAAGAGTTTGATTTTGAAGCATTACTTGTTACTCGTAGTGAACATGGTATGACATTACTA
CGCCGTAATATGGAACCATTGCATTTACCTACTCAAGCTCGTGAAGTGTTTGATGTGACAGGTGCTGGTGATACGGTTAT
TTCAGTATTGGCTGCATCAGTATCAACAGGTAAACCGTTAGATGAAGCGTGTGCATTAGCAAACGCCGCTGCGGGTGTTG
TTGTTGGTAAATTGGGTACTTCTACGCTATCAACCATTGAACTTGCAGAAGCGATTCATGGTAGCCAAGATTCTGGCTTT
GGTATTATCGGTGAAGAACAGCTGATCAGTGCGGTAAAACAAGCTCGTGCTCGTGGTGAGAAAGTCGTTATGACAAATGG
CTGTTTTGATATTTTACATGCAGGTCATGTTTCTTATTTAAATCATGCTGCTGAATTAGGTGATCGTTTGATTGTTGCTG
TTAACACTAATGAATCAGTACAACGATTAAAAGGTCCTGGTCGTCCTATTAACCCAACGGATCGTCGTATGGCGGTATTG
GCTGGTTTAGGTGCCGTTGATTGGGTTGTTCCTTTCTCTGAAGACACACCACAAAGATTGATCTCACAAGTATTGCCATC
GCTATTGGTTAAAGGTGGCGATTATGCGATCGAAGATATCGCTGGTGGTGCTGAAGTGATTGCTGCGGGTGGTGAAGTTA
AAGTATTAAACTTTGAAGATGGCTGTTCAACAACAGGTATCATTGAAGCCATTAAAGGCGGTCGCGGCTAA

Upstream 100 bases:

>100_bases
TAGAACTTGCGTAAATGCTACCATTTGTAACAGTAGATTTTTTATAAATAGCTCGAACCAATAATGGTGGATAGTTTGCT
ATTTATTGCCGGAGAAAAGA

Downstream 100 bases:

>100_bases
TAGCGGCAACGGATAAAAGAAAAGCCACATTATAGTAATGTGGCTTTTTTGTTTGTGTGCTAATTATGTAGTAATTACAT
AATTGGTATCAACTTATTTG

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKPTLPNYDQSSVLIVGDVMLDRYWGGPTSRISPEAPVPVVKVEKIEERPGGAANVAMNIAALGGDAHLVGLVGEDEPAQ
ALTTTLESLKVHCDFVALPEFPTITKLRVMSRGQQLIRLDFEDSFHDVAAEPIISRMQQALSSVKAVVLSDYAKGALEHV
QLMIQEARKVNVPVFIDPKGADFERYRGATLLTPNMLEFETVVGKVKDEDDLVAKGQQIIEEFDFEALLVTRSEHGMTLL
RRNMEPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDSGF
GIIGEEQLISAVKQARARGEKVVMTNGCFDILHAGHVSYLNHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISQVLPSLLVKGGDYAIEDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG

Sequences:

>Translated_476_residues
MKPTLPNYDQSSVLIVGDVMLDRYWGGPTSRISPEAPVPVVKVEKIEERPGGAANVAMNIAALGGDAHLVGLVGEDEPAQ
ALTTTLESLKVHCDFVALPEFPTITKLRVMSRGQQLIRLDFEDSFHDVAAEPIISRMQQALSSVKAVVLSDYAKGALEHV
QLMIQEARKVNVPVFIDPKGADFERYRGATLLTPNMLEFETVVGKVKDEDDLVAKGQQIIEEFDFEALLVTRSEHGMTLL
RRNMEPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDSGF
GIIGEEQLISAVKQARARGEKVVMTNGCFDILHAGHVSYLNHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISQVLPSLLVKGGDYAIEDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG
>Mature_476_residues
MKPTLPNYDQSSVLIVGDVMLDRYWGGPTSRISPEAPVPVVKVEKIEERPGGAANVAMNIAALGGDAHLVGLVGEDEPAQ
ALTTTLESLKVHCDFVALPEFPTITKLRVMSRGQQLIRLDFEDSFHDVAAEPIISRMQQALSSVKAVVLSDYAKGALEHV
QLMIQEARKVNVPVFIDPKGADFERYRGATLLTPNMLEFETVVGKVKDEDDLVAKGQQIIEEFDFEALLVTRSEHGMTLL
RRNMEPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDSGF
GIIGEEQLISAVKQARARGEKVVMTNGCFDILHAGHVSYLNHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISQVLPSLLVKGGDYAIEDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=472, Percent_Identity=67.5847457627119, Blast_Score=648, Evalue=0.0,
Organism=Escherichia coli, GI1788491, Length=285, Percent_Identity=25.6140350877193, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.- [C]

Molecular weight: Translated: 50752; Mature: 50752

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTLPNYDQSSVLIVGDVMLDRYWGGPTSRISPEAPVPVVKVEKIEERPGGAANVAMNI
CCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCCCHHEEEEE
AALGGDAHLVGLVGEDEPAQALTTTLESLKVHCDFVALPEFPTITKLRVMSRGQQLIRLD
EEECCCEEEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCEEEEEE
FEDSFHDVAAEPIISRMQQALSSVKAVVLSDYAKGALEHVQLMIQEARKVNVPVFIDPKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ADFERYRGATLLTPNMLEFETVVGKVKDEDDLVAKGQQIIEEFDFEALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
RRNMEPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGT
HCCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEECCCC
STLSTIELAEAIHGSQDSGFGIIGEEQLISAVKQARARGEKVVMTNGCFDILHAGHVSYL
CHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCHHHHH
NHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVLAGLGAVDWVVPFSEDTPQRL
HHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
ISQVLPSLLVKGGDYAIEDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG
HHHHHHHHHHCCCCEEHHHHCCCCEEEECCCEEEEEEECCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKPTLPNYDQSSVLIVGDVMLDRYWGGPTSRISPEAPVPVVKVEKIEERPGGAANVAMNI
CCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCCCCCHHEEEEE
AALGGDAHLVGLVGEDEPAQALTTTLESLKVHCDFVALPEFPTITKLRVMSRGQQLIRLD
EEECCCEEEEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCEEEEEE
FEDSFHDVAAEPIISRMQQALSSVKAVVLSDYAKGALEHVQLMIQEARKVNVPVFIDPKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ADFERYRGATLLTPNMLEFETVVGKVKDEDDLVAKGQQIIEEFDFEALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
RRNMEPLHLPTQAREVFDVTGAGDTVISVLAASVSTGKPLDEACALANAAAGVVVGKLGT
HCCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEECCCC
STLSTIELAEAIHGSQDSGFGIIGEEQLISAVKQARARGEKVVMTNGCFDILHAGHVSYL
CHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCHHHHH
NHAAELGDRLIVAVNTNESVQRLKGPGRPINPTDRRMAVLAGLGAVDWVVPFSEDTPQRL
HHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
ISQVLPSLLVKGGDYAIEDIAGGAEVIAAGGEVKVLNFEDGCSTTGIIEAIKGGRG
HHHHHHHHHHCCCCEEHHHHCCCCEEEECCCEEEEEEECCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA