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The map label for this gene is nudF [H]

Identifier: 59712839

GI number: 59712839

Start: 2504160

End: 2504798

Strand: Reverse

Name: nudF [H]

Synonym: VF_2232

Alternate gene names: 59712839

Gene position: 2504798-2504160 (Counterclockwise)

Preceding gene: 59712841

Following gene: 59712838

Centisome position: 86.45

GC content: 40.38

Gene sequence:

>639_bases
ATGGTTTTATCATCAAATATAGACCTAGTGAATCAGTTTGGTTCTGAAGATGTTGAAATAATATCGAAAGAGCCTTTGTT
TAATGGTTTCTTTAAAATGACAAAAGTTGCTTTTCGTCACCAATTGTTTAGCGGCGGTTGGAGTGAAGTCATTGAACGAG
AGCTTTTTGAACGAGGTCATGCGGTTGCATTATTGCCTTATGATCCTAAAACGGATCAGGTTGTATTAATTGAACAGATC
CGAGTTGGAGCATTAGAATCCAACGCACCTTGGCAGTATGAAATTGTCGCTGGAATGATCGATAAAGATGAATCAGCAGA
GCAAGTGGCCGTTCGTGAGGCCGATGAAGAAGCCGGTATAACCGTTGCTCATTTAGAGAAAATATCGCATTTTTATCCAT
CCTCGGGTGGCTGTACTGAAAGAATCGATGTTTTTGTTGGGTGTGTTGATGCTTCTAAAGCCGAAGGTATCCACGGTTTA
GAAGATGAAAACGAAGATATTCAAGTACATGTCGTTTCTCGTGAAGAGGCGTATGCTCTTGTTAATCGTGGCATAATTGA
GAATGCAGCCTCAATTATTGCCCTTCAATGGTTAGAATTAAATGTTTCGCGTTTGCGATCACAGTGGAATGCGCAATAA

Upstream 100 bases:

>100_bases
GCTTATAAAAAAAATGTAAGCATTATCAAAAAAGTTGAGTTAATAAATTGTTGCTCGTAAAAATGAGTGATAAAACAGAG
CGAGAAATTGAGAGGAATCG

Downstream 100 bases:

>100_bases
CATGCGTAAATACCATGTAGATTTAGCGAGCTTGATGAGAGTATATGAAACTAATTACGCTAAATTAGTGTCTCTTTTAC
CAAAGAAAGAAAATATAGGT

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MVLSSNIDLVNQFGSEDVEIISKEPLFNGFFKMTKVAFRHQLFSGGWSEVIERELFERGHAVALLPYDPKTDQVVLIEQI
RVGALESNAPWQYEIVAGMIDKDESAEQVAVREADEEAGITVAHLEKISHFYPSSGGCTERIDVFVGCVDASKAEGIHGL
EDENEDIQVHVVSREEAYALVNRGIIENAASIIALQWLELNVSRLRSQWNAQ

Sequences:

>Translated_212_residues
MVLSSNIDLVNQFGSEDVEIISKEPLFNGFFKMTKVAFRHQLFSGGWSEVIERELFERGHAVALLPYDPKTDQVVLIEQI
RVGALESNAPWQYEIVAGMIDKDESAEQVAVREADEEAGITVAHLEKISHFYPSSGGCTERIDVFVGCVDASKAEGIHGL
EDENEDIQVHVVSREEAYALVNRGIIENAASIIALQWLELNVSRLRSQWNAQ
>Mature_212_residues
MVLSSNIDLVNQFGSEDVEIISKEPLFNGFFKMTKVAFRHQLFSGGWSEVIERELFERGHAVALLPYDPKTDQVVLIEQI
RVGALESNAPWQYEIVAGMIDKDESAEQVAVREADEEAGITVAHLEKISHFYPSSGGCTERIDVFVGCVDASKAEGIHGL
EDENEDIQVHVVSREEAYALVNRGIIENAASIIALQWLELNVSRLRSQWNAQ

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=203, Percent_Identity=55.6650246305419, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1788810, Length=191, Percent_Identity=31.413612565445, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23673; Mature: 23673

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSSNIDLVNQFGSEDVEIISKEPLFNGFFKMTKVAFRHQLFSGGWSEVIERELFERGH
CEECCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
AVALLPYDPKTDQVVLIEQIRVGALESNAPWQYEIVAGMIDKDESAEQVAVREADEEAGI
EEEEEECCCCCCCEEEEEEHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHCCHHHCCE
TVAHLEKISHFYPSSGGCTERIDVFVGCVDASKAEGIHGLEDENEDIQVHVVSREEAYAL
EHHHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHHHHH
VNRGIIENAASIIALQWLELNVSRLRSQWNAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLSSNIDLVNQFGSEDVEIISKEPLFNGFFKMTKVAFRHQLFSGGWSEVIERELFERGH
CEECCCCHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
AVALLPYDPKTDQVVLIEQIRVGALESNAPWQYEIVAGMIDKDESAEQVAVREADEEAGI
EEEEEECCCCCCCEEEEEEHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHCCHHHCCE
TVAHLEKISHFYPSSGGCTERIDVFVGCVDASKAEGIHGLEDENEDIQVHVVSREEAYAL
EHHHHHHHHHHCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHHHHH
VNRGIIENAASIIALQWLELNVSRLRSQWNAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]