Click here to switch to the map view.

The map label for this gene is ygfZ [C]

Identifier: 59712704

GI number: 59712704

Start: 2333426

End: 2334382

Strand: Direct

Name: ygfZ [C]

Synonym: VF_2097

Alternate gene names: 59712704

Gene position: 2333426-2334382 (Clockwise)

Preceding gene: 59712701

Following gene: 59712705

Centisome position: 80.53

GC content: 38.04

Gene sequence:

>957_bases
ATGATGAGCACTCTATTCCCAGCATTAAATTTAAATAAAGATGAGCCATTACCAAGCTTCACTGTCTCCGAGTTAAATGA
TTGGGCATTAATCACAATGATTGGTGCAGATAAAAAATCATACCTACAAGGTCAAGTTACTTGCGATGTTGTTTCTTTAG
CTCAAGATGAGATCACCTTCGGTGGGCATTGTGATGCAAAAGGGAAATTATGGAGTATCTTCCAGCTCTTTCATCACAAT
GATGGCTACGCTTTATTTCAGCGTAAAAGTGCTATCGAAACAGAATTAACTGAAATAAAAAAATACGCTGTATTTTCTAA
AGTCGATATTTCAATTAGTGATGAAATTTTACTTGGTTTTACTGGCGATAAAGCCCTTGAGTGGATAAATCAACATACTG
ATTCAAATGCCAACGTCCGAGTATCTAAATTTGGTACGTTTGCCAAAGTTAGCGATACACAATGGTTACTAGTAACAACC
GATGATAAAAAAGAGGAACTTCTTTCTTTATTATCAGAAGCAACACTATGCGATGAGGCTATATGGTCTTTGCACCACAT
TAAACACGCCTTACCACAAATAGATGCCCCACTATGTAACGAACATATTCCTCAAGCACTAAATCTACAAGCCATCAATG
GTATTAGCTTTAAAAAAGGGTGCTATACAGGTCAAGAAACCGTAGCAAGAGCAAAATACCGTGGTATAAACAAACGTGCT
ATGTACTTACTTTCAGGTCTCTCAGAGGCACGACCATGTGCCGGTGATGCTATTGAGCGTAGTGTGGGTGAAAATTGGCG
TAAAGGCGGAACCATTGTTTCGGCATACCGCTTTGAAGATGGCCATACTCTTGCTCTAGCAATCCTTCCAAATGATTTAG
ATGAAGATACACAATTCAAACTTCAAGAATCTATCTGGGAAAAAGTAGAATTACCTTACTCACTTAATGATGAGTAA

Upstream 100 bases:

>100_bases
ATGAAGAAGATGCGACATGAAACTACTTGTTTCTTTTTACTGACATTTAAAATTTAGACATTTACTATAAAGAACATAAC
CTAACTTAAATAAGAATTTT

Downstream 100 bases:

>100_bases
GTTGCAAACATCTGTGACGGATCTGCTTGATAAAGAGCAGATCCCTTACCGACTACTTCCACATAAAACTCCCGCAATCA
GTGTTCAAGATGCAGCTGAA

Product: folate-dependent regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MMSTLFPALNLNKDEPLPSFTVSELNDWALITMIGADKKSYLQGQVTCDVVSLAQDEITFGGHCDAKGKLWSIFQLFHHN
DGYALFQRKSAIETELTEIKKYAVFSKVDISISDEILLGFTGDKALEWINQHTDSNANVRVSKFGTFAKVSDTQWLLVTT
DDKKEELLSLLSEATLCDEAIWSLHHIKHALPQIDAPLCNEHIPQALNLQAINGISFKKGCYTGQETVARAKYRGINKRA
MYLLSGLSEARPCAGDAIERSVGENWRKGGTIVSAYRFEDGHTLALAILPNDLDEDTQFKLQESIWEKVELPYSLNDE

Sequences:

>Translated_318_residues
MMSTLFPALNLNKDEPLPSFTVSELNDWALITMIGADKKSYLQGQVTCDVVSLAQDEITFGGHCDAKGKLWSIFQLFHHN
DGYALFQRKSAIETELTEIKKYAVFSKVDISISDEILLGFTGDKALEWINQHTDSNANVRVSKFGTFAKVSDTQWLLVTT
DDKKEELLSLLSEATLCDEAIWSLHHIKHALPQIDAPLCNEHIPQALNLQAINGISFKKGCYTGQETVARAKYRGINKRA
MYLLSGLSEARPCAGDAIERSVGENWRKGGTIVSAYRFEDGHTLALAILPNDLDEDTQFKLQESIWEKVELPYSLNDE
>Mature_318_residues
MMSTLFPALNLNKDEPLPSFTVSELNDWALITMIGADKKSYLQGQVTCDVVSLAQDEITFGGHCDAKGKLWSIFQLFHHN
DGYALFQRKSAIETELTEIKKYAVFSKVDISISDEILLGFTGDKALEWINQHTDSNANVRVSKFGTFAKVSDTQWLLVTT
DDKKEELLSLLSEATLCDEAIWSLHHIKHALPQIDAPLCNEHIPQALNLQAINGISFKKGCYTGQETVARAKYRGINKRA
MYLLSGLSEARPCAGDAIERSVGENWRKGGTIVSAYRFEDGHTLALAILPNDLDEDTQFKLQESIWEKVELPYSLNDE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=329, Percent_Identity=42.5531914893617, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_VIBF1 (Q5E304)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205480.1
- HSSP:   P0ADE8
- ProteinModelPortal:   Q5E304
- SMR:   Q5E304
- STRING:   Q5E304
- GeneID:   3279160
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2097
- NMPDR:   fig|312309.3.peg.2097
- eggNOG:   COG0354
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- PhylomeDB:   Q5E304
- ProtClustDB:   CLSK874810
- BioCyc:   VFIS312309:VF2097-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 35590; Mature: 35590

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: BINDING 28-28 BINDING 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTLFPALNLNKDEPLPSFTVSELNDWALITMIGADKKSYLQGQVTCDVVSLAQDEITF
CCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCHHHHCCCEEEEHHHHHHHCCCC
GGHCDAKGKLWSIFQLFHHNDGYALFQRKSAIETELTEIKKYAVFSKVDISISDEILLGF
CCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEE
TGDKALEWINQHTDSNANVRVSKFGTFAKVSDTQWLLVTTDDKKEELLSLLSEATLCDEA
CCHHHHHHHHHHCCCCCCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHH
IWSLHHIKHALPQIDAPLCNEHIPQALNLQAINGISFKKGCYTGQETVARAKYRGINKRA
HHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHH
MYLLSGLSEARPCAGDAIERSVGENWRKGGTIVSAYRFEDGHTLALAILPNDLDEDTQFK
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHH
LQESIWEKVELPYSLNDE
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMSTLFPALNLNKDEPLPSFTVSELNDWALITMIGADKKSYLQGQVTCDVVSLAQDEITF
CCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCHHHHCCCEEEEHHHHHHHCCCC
GGHCDAKGKLWSIFQLFHHNDGYALFQRKSAIETELTEIKKYAVFSKVDISISDEILLGF
CCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEE
TGDKALEWINQHTDSNANVRVSKFGTFAKVSDTQWLLVTTDDKKEELLSLLSEATLCDEA
CCHHHHHHHHHHCCCCCCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHH
IWSLHHIKHALPQIDAPLCNEHIPQALNLQAINGISFKKGCYTGQETVARAKYRGINKRA
HHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHH
MYLLSGLSEARPCAGDAIERSVGENWRKGGTIVSAYRFEDGHTLALAILPNDLDEDTQFK
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHH
LQESIWEKVELPYSLNDE
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA