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The map label for this gene is nadB [H]

Identifier: 59712701

GI number: 59712701

Start: 2330939

End: 2332591

Strand: Direct

Name: nadB [H]

Synonym: VF_2094

Alternate gene names: 59712701

Gene position: 2330939-2332591 (Clockwise)

Preceding gene: 59712689

Following gene: 59712704

Centisome position: 80.45

GC content: 42.59

Gene sequence:

>1653_bases
ATGATCGGATTATCACAGGAAAATGATGAATATATGAACATAACCAATGAACACAGTTGTGACGTTTTAGTCATAGGCAG
TGGTGCTGCTGGTCTTTCTCTTGCCCTTAGAGTCGCACCACATGGAAAGGTGATCGTCTTAAGTAAAGGCCCTCGAAATG
AAGGCTCAACCTATTACGCTCAAGGCGGCATAGCTGCCGTATTTGATGAAAGTGATACCGTTGAATCACATGTTGAAGAT
ACCCTTGTAGCAGGCGCGCACATATGTGACCGAGATGTCGTTACATTTATTGCAGAAAACGCAAAACATTGCGTTCAATG
GTTAATTGATGGCGGCGTTCCTTTTGATCACGATGATGAAAAAGAAACCGATATCTCCGATCCTAAATTCCATTTAACTC
GTGAAGGTGGACATAGCCACCGTCGTATTTTGCATGCTGCGGATGCGACAGGTAAAGCCATGCAAAATTCACTGCAAGAG
AATGTGCTTAACCACCCAAACATTACGATTTTTGAGCGCCACAATGCGTTGGATTTAATCACCGAAGATAAAATTGGTGG
TGATGCGGATAAAGTTGTTGGTGCTTATATTTGGAACCGTGATGCAGAACACGTTGAAACCGTTCGTGCCAAATTTGTTA
TTTTAGCCACTGGCGGTGCATCTAAAGTATACCAATACACATCAAACCCAGATGTATCTTCAGGTGATGGTATTGCAATG
GCATGGCGTGCTGGTTGTCGAGTTGCTAACTTAGAATTTAACCAATTCCACCCAACCTGTCTTTTCCACCCTGACGCACG
AAACTTCTTAATGACAGAAGCATTACGTGGTGAAGGCGCTTATTTACGCCGCCCAGATGGTACTCGTTTTATGCCAGATT
TCGACGAGCGTGGTGAGCTTGCTCCTCGTGATGTTGTCGCTCGAGCTATCGATTACGAAATGAAGCGTTTAGGGGCTGAC
TGCATGTATTTAGACATCAGCCATAAGCCTGAAGACTTCATTCACCACCATTTCCCTACCATTAATGAAAAGCTGCTTTC
ATTTGGTATTGATATGACAAAAGAACCAATCCCTATCGTACCTGCTGCTCACTATACCTGTGGCGGTGTAATGGTTGATA
AACAAGGCCAAACCGATATTGATGGTCTATTTGCTATTGGTGAAGTAAGTTACACCGGTCTTCATGGTGCAAACCGTTTA
GCATCAAACTCACTATTAGAGTGCGTTGTGTATGCGTGGTCAGCGGCTGAATATATTATTAAAGATATGGATAAACGCGA
ACTGCCACCAACGCTGCCAGCATGGGATGAAAGCCAAGTTGAATGCTCTGATGAAGAAGTAGTTATTCAGCATAACTGGC
ACGAACTACGCCTATTCATGTGGGATTACATGGGTATCGTTCGTACTGACAAACGTCTAGAACGTGCACTGCGCCGTATT
CAATTACTAAAACAAGAAACTCATGATTATTATAGTAATTTCAGAGTTTCGAATAACTTAATTGAATTACGAAATCTTTT
AGACGTATCGGAATTGATGGTACGTTGTGCAATGAAACGTAAAGAAAGTTGTGGTTTACACTATACGCTTGACTATCCAG
AGGCATTGGAAGAGTCAGGTCCAACTATTTTAGTGCCAACCAACTTTAAGTAG

Upstream 100 bases:

>100_bases
TGGGACTTTAAAATCCTTTTTGCTGATATTTACGAACTTTTATTCTTGATATTTAAGATTCCATTCCAAAATGTGACGCA
ACCGGATAGATTCTGGTAAA

Downstream 100 bases:

>100_bases
CGAAACCTTACTTAATAACGACAACACGACTACTTTAAGAGCACCATTAATTGATGGTGCTTTTTTTTATCTATCGCATC
TGAGAATAAATAAAAGAACT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MIGLSQENDEYMNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDESDTVESHVED
TLVAGAHICDRDVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISDPKFHLTREGGHSHRRILHAADATGKAMQNSLQE
NVLNHPNITIFERHNALDLITEDKIGGDADKVVGAYIWNRDAEHVETVRAKFVILATGGASKVYQYTSNPDVSSGDGIAM
AWRAGCRVANLEFNQFHPTCLFHPDARNFLMTEALRGEGAYLRRPDGTRFMPDFDERGELAPRDVVARAIDYEMKRLGAD
CMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRL
ASNSLLECVVYAWSAAEYIIKDMDKRELPPTLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRI
QLLKQETHDYYSNFRVSNNLIELRNLLDVSELMVRCAMKRKESCGLHYTLDYPEALEESGPTILVPTNFK

Sequences:

>Translated_550_residues
MIGLSQENDEYMNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDESDTVESHVED
TLVAGAHICDRDVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISDPKFHLTREGGHSHRRILHAADATGKAMQNSLQE
NVLNHPNITIFERHNALDLITEDKIGGDADKVVGAYIWNRDAEHVETVRAKFVILATGGASKVYQYTSNPDVSSGDGIAM
AWRAGCRVANLEFNQFHPTCLFHPDARNFLMTEALRGEGAYLRRPDGTRFMPDFDERGELAPRDVVARAIDYEMKRLGAD
CMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRL
ASNSLLECVVYAWSAAEYIIKDMDKRELPPTLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRI
QLLKQETHDYYSNFRVSNNLIELRNLLDVSELMVRCAMKRKESCGLHYTLDYPEALEESGPTILVPTNFK
>Mature_550_residues
MIGLSQENDEYMNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYAQGGIAAVFDESDTVESHVED
TLVAGAHICDRDVVTFIAENAKHCVQWLIDGGVPFDHDDEKETDISDPKFHLTREGGHSHRRILHAADATGKAMQNSLQE
NVLNHPNITIFERHNALDLITEDKIGGDADKVVGAYIWNRDAEHVETVRAKFVILATGGASKVYQYTSNPDVSSGDGIAM
AWRAGCRVANLEFNQFHPTCLFHPDARNFLMTEALRGEGAYLRRPDGTRFMPDFDERGELAPRDVVARAIDYEMKRLGAD
CMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIVPAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRL
ASNSLLECVVYAWSAAEYIIKDMDKRELPPTLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRI
QLLKQETHDYYSNFRVSNNLIELRNLLDVSELMVRCAMKRKESCGLHYTLDYPEALEESGPTILVPTNFK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=521, Percent_Identity=32.0537428023033, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1788928, Length=538, Percent_Identity=73.7918215613383, Blast_Score=829, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=532, Percent_Identity=33.0827067669173, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI1786942, Length=561, Percent_Identity=31.1942959001783, Blast_Score=201, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17550100, Length=539, Percent_Identity=30.7977736549165, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17505833, Length=538, Percent_Identity=29.368029739777, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71986328, Length=303, Percent_Identity=27.3927392739274, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322701, Length=555, Percent_Identity=32.6126126126126, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=572, Percent_Identity=30.4195804195804, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320788, Length=492, Percent_Identity=25, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137288, Length=559, Percent_Identity=31.1270125223614, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24655642, Length=559, Percent_Identity=31.1270125223614, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24655647, Length=559, Percent_Identity=31.1270125223614, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24663005, Length=574, Percent_Identity=28.7456445993031, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 61728; Mature: 61728

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLSQENDEYMNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYA
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEE
QGGIAAVFDESDTVESHVEDTLVAGAHICDRDVVTFIAENAKHCVQWLIDGGVPFDHDDE
CCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KETDISDPKFHLTREGGHSHRRILHAADATGKAMQNSLQENVLNHPNITIFERHNALDLI
CCCCCCCCCEEEEECCCCCHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEE
TEDKIGGDADKVVGAYIWNRDAEHVETVRAKFVILATGGASKVYQYTSNPDVSSGDGIAM
ECCCCCCCHHHHHHHHEECCCHHHHHHHEEEEEEEEECCCCEEEEECCCCCCCCCCCEEE
AWRAGCRVANLEFNQFHPTCLFHPDARNFLMTEALRGEGAYLRRPDGTRFMPDFDERGEL
EECCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
APRDVVARAIDYEMKRLGADCMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIV
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
PAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVYAWSAAEYII
CCCCEECCCEEECCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KDMDKRELPPTLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRI
HCCHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLKQETHDYYSNFRVSNNLIELRNLLDVSELMVRCAMKRKESCGLHYTLDYPEALEESG
HHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCC
PTILVPTNFK
CEEEEECCCC
>Mature Secondary Structure
MIGLSQENDEYMNITNEHSCDVLVIGSGAAGLSLALRVAPHGKVIVLSKGPRNEGSTYYA
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEE
QGGIAAVFDESDTVESHVEDTLVAGAHICDRDVVTFIAENAKHCVQWLIDGGVPFDHDDE
CCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KETDISDPKFHLTREGGHSHRRILHAADATGKAMQNSLQENVLNHPNITIFERHNALDLI
CCCCCCCCCEEEEECCCCCHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEE
TEDKIGGDADKVVGAYIWNRDAEHVETVRAKFVILATGGASKVYQYTSNPDVSSGDGIAM
ECCCCCCCHHHHHHHHEECCCHHHHHHHEEEEEEEEECCCCEEEEECCCCCCCCCCCEEE
AWRAGCRVANLEFNQFHPTCLFHPDARNFLMTEALRGEGAYLRRPDGTRFMPDFDERGEL
EECCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
APRDVVARAIDYEMKRLGADCMYLDISHKPEDFIHHHFPTINEKLLSFGIDMTKEPIPIV
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
PAAHYTCGGVMVDKQGQTDIDGLFAIGEVSYTGLHGANRLASNSLLECVVYAWSAAEYII
CCCCEECCCEEECCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KDMDKRELPPTLPAWDESQVECSDEEVVIQHNWHELRLFMWDYMGIVRTDKRLERALRRI
HCCHHHCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLLKQETHDYYSNFRVSNNLIELRNLLDVSELMVRCAMKRKESCGLHYTLDYPEALEESG
HHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCC
PTILVPTNFK
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]