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The map label for this gene is pyrG [H]

Identifier: 59712683

GI number: 59712683

Start: 2310798

End: 2312438

Strand: Reverse

Name: pyrG [H]

Synonym: VF_2076

Alternate gene names: 59712683

Gene position: 2312438-2310798 (Counterclockwise)

Preceding gene: 172087751

Following gene: 59712682

Centisome position: 79.81

GC content: 42.47

Gene sequence:

>1641_bases
ATGACGACGAATTACATTTTTGTAACTGGCGGGGTTGTATCCTCACTAGGTAAAGGTATTGCAGCAGCATCATTAGCAGC
TATTCTTGAAGCTCGTGGCTTAAAAGTCACTATGATGAAGCTTGACCCATACATCAACGTTGATCCAGGCACAATGAGCC
CAACTCAACACGGTGAAGTATTCGTCACGGAAGACGGCGCAGAAACGGATCTAGACTTAGGTCACTACGAGCGCTTCATC
CGCACCAAAATGACTAAGCGTAACAACTTTACAGCTGGTCGAGTATACGCAGATGTTCTACGTAAAGAACGTCGTGGCGA
CTACTTAGGTGCGACTATCCAAGTTATCCCTCACATTACTAATGCGATTAAAGAACGTGTTATCTCTGGCGCTGAAGGCC
ACGATATCGCAATCGTAGAAGTTGGTGGTACAGTTGGTGATATCGAATCTCTACCTTTCATGGAAGCGATTCGTCAACTA
GCAGTAGAACTAGGCCGTGAGCGTGCAATGTTTATGCACCTAACACTGGTTCCTTACCTAGCCGCTGCTGGTGAAGTGAA
AACGAAACCAACTCAACACTCAGTTAAAGAACTACTATCTATCGGTATCCAACCTGATATCTTAGTTTGTCGTTCTGACC
GTGTTATCCCAGCAAACGAACGTAAGAAAATTGCTCTTTTCTGTAACGTACAAGAAAAAGCAGTTATCTCAATGAAAGAC
GTAGATTCAATCTACAAAATCCCACAACTTATCAAAGCTCAAGGTACTGATGACTTAGTATGTCAACGCTTTGGTATTAC
AGCACCTGAAGCTGATCTTTCTGAGTGGGAACAAGTAATTTATGAAGAAGCTAACCCAACTGGTGATGTTGTTATCGGTA
TGGTTGGTAAATACATTGAATTACCAGATGCTTATAAGTCAGTAAACGAAGCATTAAAACATGCGGGCTTGAAAAATCGT
TTAAGCGTCACTATTAAGTATGTAGATTCACAAGATGTAGAATCTAAAGGTACTGAAATCCTTGAAGGTCTGGATGCAAT
TCTAGTTCCTGGTGGTTTCGGTGACCGCGGTGTTGAAGGTAAGATCCTTGCAGCACAATACGCTCGTGAAAACAAAGTAC
CATATTTAGGAATCTGTCTAGGTATGCAAGTTGCGTTAATTGAATATGCGCGTAACGTTGCTAATATGGAAGGGGCACAC
TCTTCTGAATTCTCTAAAGACACGAAATTCCCAGTTGTTGGTCTTATCACTGAGTGGATTGACAGCGAAGGTAATGTTGA
AGAGCGTACTGAGAAATCAGATCTTGGCGGTACAATGCGTCTTGGTTCTCAACTATGTCATCTTGCTAAAGGTTCAAAAG
CTCGTGAGCTTTACGGTAATGCAACAGTTGAAGAGCGTCACCGTCACCGTTATGAAGTAAACAACAACTTACTTCCTCAG
TTAGAAAAAGCAGGCCTTAAGGTTTCTGGTTTATCTGCTGATAAGAAGCTGGTGGAAATTATTGAAATCCCTAACCACCC
ATGGTTTGTGGCAGCTCAATTCCACCCAGAATTCACATCAACACCTCGTGATGGTCACCCATTATTTGAAGGTTTTGTTA
AAGCTGCAGGCGAAAGCGTGCGCGGTGAATTAGAAAAGTAA

Upstream 100 bases:

>100_bases
ATACTTTTCACGTTGTGGCGATAAAGCGGTTCTGGTATATTCACTTCCCGTCCAGATGAAATCCATTTTTATTCCTCATT
ACACCAATTTCAGGTAACGT

Downstream 100 bases:

>100_bases
TAGGATTTACGAGTAGCTGTCCAATGTTGGGCGGCTATTTTTAGCTTTTAAATTAAAGAAAAGTAAAGAGGAAACACAAT
GTCTAAGATCGTTAAAGTTC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKERVISGAEGHDIAIVEVGGTVGDIESLPFMEAIRQL
AVELGRERAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
VDSIYKIPQLIKAQGTDDLVCQRFGITAPEADLSEWEQVIYEEANPTGDVVIGMVGKYIELPDAYKSVNEALKHAGLKNR
LSVTIKYVDSQDVESKGTEILEGLDAILVPGGFGDRGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVANMEGAH
SSEFSKDTKFPVVGLITEWIDSEGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
LEKAGLKVSGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGESVRGELEK

Sequences:

>Translated_546_residues
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKERVISGAEGHDIAIVEVGGTVGDIESLPFMEAIRQL
AVELGRERAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
VDSIYKIPQLIKAQGTDDLVCQRFGITAPEADLSEWEQVIYEEANPTGDVVIGMVGKYIELPDAYKSVNEALKHAGLKNR
LSVTIKYVDSQDVESKGTEILEGLDAILVPGGFGDRGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVANMEGAH
SSEFSKDTKFPVVGLITEWIDSEGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
LEKAGLKVSGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGESVRGELEK
>Mature_545_residues
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHITNAIKERVISGAEGHDIAIVEVGGTVGDIESLPFMEAIRQLA
VELGRERAMFMHLTLVPYLAAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKDV
DSIYKIPQLIKAQGTDDLVCQRFGITAPEADLSEWEQVIYEEANPTGDVVIGMVGKYIELPDAYKSVNEALKHAGLKNRL
SVTIKYVDSQDVESKGTEILEGLDAILVPGGFGDRGVEGKILAAQYARENKVPYLGICLGMQVALIEYARNVANMEGAHS
SEFSKDTKFPVVGLITEWIDSEGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQL
EKAGLKVSGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGESVRGELEK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI28559085, Length=566, Percent_Identity=44.3462897526502, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=566, Percent_Identity=44.3462897526502, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=566, Percent_Identity=44.3462897526502, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI148491070, Length=556, Percent_Identity=44.2446043165468, Blast_Score=469, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=79.9256505576208, Blast_Score=892, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=610, Percent_Identity=39.672131147541, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319432, Length=572, Percent_Identity=42.1328671328671, Blast_Score=448, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322563, Length=567, Percent_Identity=41.6225749559083, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=556, Percent_Identity=43.8848920863309, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=41.8326693227092, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59958; Mature: 59827

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKERVISGAEGHDIAIVEVGGTVGDIESLPFMEAIRQLAVELGRERAMFMHLTLVPYL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHEEEHHH
VDSIYKIPQLIKAQGTDDLVCQRFGITAPEADLSEWEQVIYEEANPTGDVVIGMVGKYIE
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHC
LPDAYKSVNEALKHAGLKNRLSVTIKYVDSQDVESKGTEILEGLDAILVPGGFGDRGVEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCC
KILAAQYARENKVPYLGICLGMQVALIEYARNVANMEGAHSSEFSKDTKFPVVGLITEWI
CEEEHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DSEGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHH
LEKAGLKVSGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGESV
HHHCCCEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHH
RGELEK
HCCCCC
>Mature Secondary Structure 
TTNYIFVTGGVVSSLGKGIAAASLAAILEARGLKVTMMKLDPYINVDPGTMSPTQHGEV
CCCEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTKRNNFTAGRVYADVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKERVISGAEGHDIAIVEVGGTVGDIESLPFMEAIRQLAVELGRERAMFMHLTLVPYL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AAAGEVKTKPTQHSVKELLSIGIQPDILVCRSDRVIPANERKKIALFCNVQEKAVISMKD
HHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHEEEHHH
VDSIYKIPQLIKAQGTDDLVCQRFGITAPEADLSEWEQVIYEEANPTGDVVIGMVGKYIE
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHC
LPDAYKSVNEALKHAGLKNRLSVTIKYVDSQDVESKGTEILEGLDAILVPGGFGDRGVEG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCC
KILAAQYARENKVPYLGICLGMQVALIEYARNVANMEGAHSSEFSKDTKFPVVGLITEWI
CEEEHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DSEGNVEERTEKSDLGGTMRLGSQLCHLAKGSKARELYGNATVEERHRHRYEVNNNLLPQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHH
LEKAGLKVSGLSADKKLVEIIEIPNHPWFVAAQFHPEFTSTPRDGHPLFEGFVKAAGESV
HHHCCCEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCHHH
RGELEK
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA