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The map label for this gene is mazG [H]

Identifier: 172087751

GI number: 172087751

Start: 2312570

End: 2313391

Strand: Reverse

Name: mazG [H]

Synonym: VF_2078

Alternate gene names: 172087751

Gene position: 2313391-2312570 (Counterclockwise)

Preceding gene: 59712686

Following gene: 59712683

Centisome position: 79.84

GC content: 36.25

Gene sequence:

>822_bases
ATGACTACCCCAAATAATATTGAACAGTTACTCTCTATCATGACTCAACTAAGAGACGAAAAGAAAGGGTGCCCTTGGGA
TAAAAAACAAACCTTTGATTCTATTGTTCCGCATACCATAGAAGAAACATATGAAGTGGTGGATGCAATTCATAAAAAGG
ACTGGAGTAATTTAAAAGAAGAGTTGGGAGATTTACTGTTTCAAGTGGTTTTCTATGCTCAAATCGCTCAAGAAGAGCAA
TTGTTCGATTTTAATGACGTACTAGAAACATTAAACGCAAAATTGATCCGTCGTCATCCACACGTGTTCGGTGAAGAGAA
TTTTTCATCTGATGCTGAAATTGCAGCTAATTGGGATAAAGTAAAACAGCAAGAGCGTTTAGATAAAGAGAAAAGCAACC
AACAAACGACTGTTCAAGAAAGTATTCTAGATACGATCCCAACGGCTTTACCAAGTTTGTTAAGAGCAAATAAAATACAA
AAACAGTGTGCTAAAGTTGGCTTTGACTGGGATGAGTTACAACCTGTTGCAAATAAAGTAAATGAAGAAGTGGCGGAAGT
GCTAGAAGAGGTCTATAAAGTACCCAGAAATGAAGAAAAAGTAGAAGAAGAATTAGGCGATCTACTTTTTGCCACGGTAA
ATCTAGTGCGTCATTTAGAAAAAAATCCAGAGCAAGTTTTAAGCAAAGCAAATAACAAATTTGAGCGTCGTTTTCGTCAA
ATTGAAGCGAACGTCGCTAAAAAAGGCAAAAAAATAGAGCAATGTGAATTAAATGAGCTAGATTTGGAGTGGGAAAAAAT
TAAACGAAGTGAGCGGAATTGA

Upstream 100 bases:

>100_bases
GGCTAATTTGCCAAAAAATTAAAATTCATCGAGATTAAGAAAGCTGTGACTATAATGGTCACAGCTTTTTTTGTTTTTAT
ACCAATCACAGGGAATAAAT

Downstream 100 bases:

>100_bases
TTTGGAGCAAAAAATAAATCAATTAACTGTGATACTTTTCACGTTGTGGCGATAAAGCGGTTCTGGTATATTCACTTCCC
GTCCAGATGAAATCCATTTT

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTTPNNIEQLLSIMTQLRDEKKGCPWDKKQTFDSIVPHTIEETYEVVDAIHKKDWSNLKEELGDLLFQVVFYAQIAQEEQ
LFDFNDVLETLNAKLIRRHPHVFGEENFSSDAEIAANWDKVKQQERLDKEKSNQQTTVQESILDTIPTALPSLLRANKIQ
KQCAKVGFDWDELQPVANKVNEEVAEVLEEVYKVPRNEEKVEEELGDLLFATVNLVRHLEKNPEQVLSKANNKFERRFRQ
IEANVAKKGKKIEQCELNELDLEWEKIKRSERN

Sequences:

>Translated_273_residues
MTTPNNIEQLLSIMTQLRDEKKGCPWDKKQTFDSIVPHTIEETYEVVDAIHKKDWSNLKEELGDLLFQVVFYAQIAQEEQ
LFDFNDVLETLNAKLIRRHPHVFGEENFSSDAEIAANWDKVKQQERLDKEKSNQQTTVQESILDTIPTALPSLLRANKIQ
KQCAKVGFDWDELQPVANKVNEEVAEVLEEVYKVPRNEEKVEEELGDLLFATVNLVRHLEKNPEQVLSKANNKFERRFRQ
IEANVAKKGKKIEQCELNELDLEWEKIKRSERN
>Mature_272_residues
TTPNNIEQLLSIMTQLRDEKKGCPWDKKQTFDSIVPHTIEETYEVVDAIHKKDWSNLKEELGDLLFQVVFYAQIAQEEQL
FDFNDVLETLNAKLIRRHPHVFGEENFSSDAEIAANWDKVKQQERLDKEKSNQQTTVQESILDTIPTALPSLLRANKIQK
QCAKVGFDWDELQPVANKVNEEVAEVLEEVYKVPRNEEKVEEELGDLLFATVNLVRHLEKNPEQVLSKANNKFERRFRQI
EANVAKKGKKIEQCELNELDLEWEKIKRSERN

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=267, Percent_Identity=52.8089887640449, Blast_Score=294, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 31902; Mature: 31771

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCHHHCCCHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
TTPNNIEQLLSIMTQLRDEKKGCPWDKKQTFDSIVPHTIEETYEVVDAIHKKDWSNLKE
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ELGDLLFQVVFYAQIAQEEQLFDFNDVLETLNAKLIRRHPHVFGEENFSSDAEIAANWDK
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
VKQQERLDKEKSNQQTTVQESILDTIPTALPSLLRANKIQKQCAKVGFDWDELQPVANKV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NEEVAEVLEEVYKVPRNEEKVEEELGDLLFATVNLVRHLEKNPEQVLSKANNKFERRFRQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IEANVAKKGKKIEQCELNELDLEWEKIKRSERN
HHHHHHHCCCCHHHCCCHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]