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The map label for this gene is eno [H]

Identifier: 59712682

GI number: 59712682

Start: 2309421

End: 2310719

Strand: Reverse

Name: eno [H]

Synonym: VF_2075

Alternate gene names: 59712682

Gene position: 2310719-2309421 (Counterclockwise)

Preceding gene: 59712683

Following gene: 59712681

Centisome position: 79.75

GC content: 44.34

Gene sequence:

>1299_bases
ATGTCTAAGATCGTTAAAGTTCTAGGTCGTGAAATCATCGATTCTCGTGGTAACCCAACAGTTGAAGCTGAAGTTCACCT
AGAAAGTGGTTTTGTAGGTATGGCTGCTGCTCCATCTGGCGCATCTACAGGTTCTCGTGAAGCTCTTGAGCTACGTGATG
GCGACAAAGCACGTTTCCTAGGTAAAGGTGTTCTTAAAGCTGTTGCAGCTGTAAATGGTCCAATCGCTGAAGCTCTAGTT
GGTAAAGACGCGAAAAACCAAGCTGAAATCGATCAAATCATGATCGACCTAGACGGTACAGACAACAAAGCAAACTTCGG
TGCTAACGCAATCCTTGCTGTTTCTCTAGCAAATGCTAAAGCTGCTGCAGCTGCTAAATCTATGCCTCTATACGCTCACA
TTGCTGAACTAAACGGCACTCCAGGCGTATTCTCTATGCCTCTACCAATGATGAACATCATCAACGGTGGTGAGCACGCT
GATAACAACGTTGACATCCAAGAGTTCATGATCCAACCAGTTGGCGCTAAAACTCTTAAAGAAGGCCTACGTATCGGTGC
TGAAGTATTCCACAACCTAGCTAAAGTACTTAAGTCTAAAGGCTACAGCACTGCAGTTGGTGACGAAGGTGGTTTCGCTC
CTAACCTTAAGTCTAACGCTGAAGCTCTAGAAGTTATCGCAGAAGCTGTTGCAGCTGCTGGTTACGAACTAGGTAAAGAC
GTTACTCTTGCTATGGACTGTGCAGCATCTGAGTTCTTCGACAAAGAAGCTGGCATCTACAACATGAAGGGCGAAGGTAA
AACTTTCACTTCTGAAGAGTTCAACCATTACCTAGCTGAGCTAGCTAACCAATTCCCAATCGTTTCTATCGAAGATGGTC
TAGACGAGTCTGATTGGACTGGCTTCAAGCACCAAACTGAACTACTAGGTGACAAACTTCAACTAGTAGGTGACGATCTA
TTCGTTACTAACACTAAGATCCTTGCTGAAGGTATCGAGAAAGGCGTAGCTAACTCTATCCTTATCAAGTTCAACCAAAT
CGGTTCTCTAACTGAGACTCTAGCTGCAATCAAGATGGCTAAAGACGCAGGTTACACAGCTGTAATCTCTCACCGTTCTG
GCGAAACTGAAGATGCAACTATTGCTGATCTAGCAGTAGGTACAGCTGCAGGTCAAATCAAAACTGGTTCTATGAGCCGT
TCTGACCGTGTTGCTAAGTACAACCAACTAATCCGTATCGAAGAAGCTCTAGGTGAAAAAGCACCTTTCAACGGTCTTAA
AGAAGTTAAAGGTCAATAA

Upstream 100 bases:

>100_bases
GCGCGGTGAATTAGAAAAGTAATAGGATTTACGAGTAGCTGTCCAATGTTGGGCGGCTATTTTTAGCTTTTAAATTAAAG
AAAAGTAAAGAGGAAACACA

Downstream 100 bases:

>100_bases
TCTCAATTTTTGAAGATTTGATTTTTAATTCTTAATCGTTCGATTAGAATTTCGATATAAAACCGTCTCATTTGAGGCGG
TTTTTTTTATTTCTGTCAGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKARFLGKGVLKAVAAVNGPIAEALV
GKDAKNQAEIDQIMIDLDGTDNKANFGANAILAVSLANAKAAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
VTLAMDCAASEFFDKEAGIYNMKGEGKTFTSEEFNHYLAELANQFPIVSIEDGLDESDWTGFKHQTELLGDKLQLVGDDL
FVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGEKAPFNGLKEVKGQ

Sequences:

>Translated_432_residues
MSKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKARFLGKGVLKAVAAVNGPIAEALV
GKDAKNQAEIDQIMIDLDGTDNKANFGANAILAVSLANAKAAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
VTLAMDCAASEFFDKEAGIYNMKGEGKTFTSEEFNHYLAELANQFPIVSIEDGLDESDWTGFKHQTELLGDKLQLVGDDL
FVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGEKAPFNGLKEVKGQ
>Mature_431_residues
SKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKARFLGKGVLKAVAAVNGPIAEALVG
KDAKNQAEIDQIMIDLDGTDNKANFGANAILAVSLANAKAAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLKEGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKDV
TLAMDCAASEFFDKEAGIYNMKGEGKTFTSEEFNHYLAELANQFPIVSIEDGLDESDWTGFKHQTELLGDKLQLVGDDLF
VTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRVAKYNQLIRIEEALGEKAPFNGLKEVKGQ

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=53.0092592592593, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=53.0092592592593, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=50.6944444444444, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=51.3888888888889, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=48.8372093023256, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=350, Percent_Identity=28.2857142857143, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI169201757, Length=350, Percent_Identity=28.2857142857143, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI239744207, Length=350, Percent_Identity=28.2857142857143, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1789141, Length=433, Percent_Identity=86.1431870669746, Blast_Score=735, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=51.7321016166282, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=51.7321016166282, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=45.3125, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.1916859122402, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=46.0829493087558, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=46.0829493087558, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=46.0829493087558, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=48.2678983833718, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580918, Length=437, Percent_Identity=50.1144164759725, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=437, Percent_Identity=50.1144164759725, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=437, Percent_Identity=50.1144164759725, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=437, Percent_Identity=50.1144164759725, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50.2325581395349, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50.2325581395349, Blast_Score=381, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45531; Mature: 45400

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKARFL
CHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEECCCCCHHHHH
GKGVLKAVAAVNGPIAEALVGKDAKNQAEIDQIMIDLDGTDNKANFGANAILAVSLANAK
HHHHHHHHHHHCCHHHHHHHCCCCCCHHCEEEEEEEECCCCCCCCCCCCEEEEEEECCCH
AAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHHCCCCEEEHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
VTLAMDCAASEFFDKEAGIYNMKGEGKTFTSEEFNHYLAELANQFPIVSIEDGLDESDWT
EEEEEHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GFKHQTELLGDKLQLVGDDLFVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMA
CCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGEK
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APFNGLKEVKGQ
CCCCCHHHHCCC
>Mature Secondary Structure 
SKIVKVLGREIIDSRGNPTVEAEVHLESGFVGMAAAPSGASTGSREALELRDGDKARFL
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEECCCCCHHHHH
GKGVLKAVAAVNGPIAEALVGKDAKNQAEIDQIMIDLDGTDNKANFGANAILAVSLANAK
HHHHHHHHHHHCCHHHHHHHCCCCCCHHCEEEEEEEECCCCCCCCCCCCEEEEEEECCCH
AAAAAKSMPLYAHIAELNGTPGVFSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHHCCCCEEEHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EGLRIGAEVFHNLAKVLKSKGYSTAVGDEGGFAPNLKSNAEALEVIAEAVAAAGYELGKD
HHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
VTLAMDCAASEFFDKEAGIYNMKGEGKTFTSEEFNHYLAELANQFPIVSIEDGLDESDWT
EEEEEHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GFKHQTELLGDKLQLVGDDLFVTNTKILAEGIEKGVANSILIKFNQIGSLTETLAAIKMA
CCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGEK
HCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APFNGLKEVKGQ
CCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA