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The map label for this gene is pcm

Identifier: 59712676

GI number: 59712676

Start: 2305294

End: 2305920

Strand: Reverse

Name: pcm

Synonym: VF_2069

Alternate gene names: 59712676

Gene position: 2305920-2305294 (Counterclockwise)

Preceding gene: 59712677

Following gene: 59712675

Centisome position: 79.58

GC content: 38.92

Gene sequence:

>627_bases
ATGCTTAATTCGCGTTCTGAATTACTAGATCAATTTTTGCGCCAACAAGGCATTAGAGACGAAGCTATTTTAGCGGCTAT
TCGAGAATTACCAAGAGAGCGTTTTATCCCAGAAGCTTTATCACATCAAGCTTATCAAAATAACGCACTTCCTATTGGTG
AGGGTCAAACGATCTCACAGCCTTATATTGTAGCGAAAATGACTGAATTATTAGAGCTAACACCTACATCAAATGTACTT
GAAGTGGGGACAGGCTCTGGTTATCAAACGGCTGTATTGGCTAAATTAGTTGAGCATGTTAACTCTATTGAGCGTATAAA
ATCGTTACAATGGAATGCGAAACGATTGTTGAAACAATTGGATATATATAATGTCTCAACAAAGCATGGTGATGGCTGGA
AGGGATGGGAGTCAAAAGCTCCATTTGATGCCATCATTGTTACTGCAGCAGCGGAATCTATTCCGAATGACTTGTTATTT
CAATTAAAAGATAATGGACATTTAGTGATCCCTATAGGGGAAGAGTCACAGCAGTTATTACGAATTACTCGTCAGGGAGA
GGAATTTTTCTCTGAAGTGATTGAAGAGGTTCGTTTTGTGCCTTTAGTTGCGGGTGAATTAGCATAA

Upstream 100 bases:

>100_bases
AGCAGCACCGAGTATCAATAACACCATTACAAGTCGATTTAACGGCTCATGATTCATTAGGTATGATTACTGATTGGTTA
TCATTAGAGAGTTCTAAATA

Downstream 100 bases:

>100_bases
TATAATGAGAAAAATGATTATCAACCTTATTTCTATACGCTTATGGTTAATGGCTGCTGTTGTTAGCTTATTACTTTCTG
GTTGTTCTATGGGACCACAT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MLNSRSELLDQFLRQQGIRDEAILAAIRELPRERFIPEALSHQAYQNNALPIGEGQTISQPYIVAKMTELLELTPTSNVL
EVGTGSGYQTAVLAKLVEHVNSIERIKSLQWNAKRLLKQLDIYNVSTKHGDGWKGWESKAPFDAIIVTAAAESIPNDLLF
QLKDNGHLVIPIGEESQQLLRITRQGEEFFSEVIEEVRFVPLVAGELA

Sequences:

>Translated_208_residues
MLNSRSELLDQFLRQQGIRDEAILAAIRELPRERFIPEALSHQAYQNNALPIGEGQTISQPYIVAKMTELLELTPTSNVL
EVGTGSGYQTAVLAKLVEHVNSIERIKSLQWNAKRLLKQLDIYNVSTKHGDGWKGWESKAPFDAIIVTAAAESIPNDLLF
QLKDNGHLVIPIGEESQQLLRITRQGEEFFSEVIEEVRFVPLVAGELA
>Mature_208_residues
MLNSRSELLDQFLRQQGIRDEAILAAIRELPRERFIPEALSHQAYQNNALPIGEGQTISQPYIVAKMTELLELTPTSNVL
EVGTGSGYQTAVLAKLVEHVNSIERIKSLQWNAKRLLKQLDIYNVSTKHGDGWKGWESKAPFDAIIVTAAAESIPNDLLF
QLKDNGHLVIPIGEESQQLLRITRQGEEFFSEVIEEVRFVPLVAGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=180, Percent_Identity=31.6666666666667, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789100, Length=209, Percent_Identity=63.6363636363636, Blast_Score=274, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71983477, Length=216, Percent_Identity=28.7037037037037, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17981723, Length=185, Percent_Identity=30.8108108108108, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_VIBF1 (Q5E332)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205452.1
- HSSP:   Q8TZR3
- ProteinModelPortal:   Q5E332
- SMR:   Q5E332
- STRING:   Q5E332
- GeneID:   3277715
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_2069
- NMPDR:   fig|312309.3.peg.2067
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- PhylomeDB:   Q5E332
- ProtClustDB:   PRK00312
- BioCyc:   VFIS312309:VF2069-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23301; Mature: 23301

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 59-59

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSRSELLDQFLRQQGIRDEAILAAIRELPRERFIPEALSHQAYQNNALPIGEGQTISQ
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
PYIVAKMTELLELTPTSNVLEVGTGSGYQTAVLAKLVEHVNSIERIKSLQWNAKRLLKQL
CHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DIYNVSTKHGDGWKGWESKAPFDAIIVTAAAESIPNDLLFQLKDNGHLVIPIGEESQQLL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEECCCCEEEEEECCCHHHHH
RITRQGEEFFSEVIEEVRFVPLVAGELA
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNSRSELLDQFLRQQGIRDEAILAAIRELPRERFIPEALSHQAYQNNALPIGEGQTISQ
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
PYIVAKMTELLELTPTSNVLEVGTGSGYQTAVLAKLVEHVNSIERIKSLQWNAKRLLKQL
CHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DIYNVSTKHGDGWKGWESKAPFDAIIVTAAAESIPNDLLFQLKDNGHLVIPIGEESQQLL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEECCCCEEEEEECCCHHHHH
RITRQGEEFFSEVIEEVRFVPLVAGELA
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA