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The map label for this gene is glnT [H]

Identifier: 59712666

GI number: 59712666

Start: 2293595

End: 2295064

Strand: Direct

Name: glnT [H]

Synonym: VF_2059

Alternate gene names: 59712666

Gene position: 2293595-2295064 (Clockwise)

Preceding gene: 59712661

Following gene: 59712670

Centisome position: 79.16

GC content: 41.77

Gene sequence:

>1470_bases
ATGACAAACACAGATTCAACGCTTATTGAATCGATGAACGAAGTATTACTTTCGATTATCGGTAGTATCAATGGGCTTCT
TTGGGGACAAGTCCTTGTTTATTTATTAGTTGGTGTGGGTGTCTACTTCACTCTGCGTCTTGGCTTTATTCAAATTCGCC
AACTAGGACACTCAATCAAAATCTTACGTAGCGGCCAAGAAATCGAAAATGGCATCAGCTCTTACCAAGTATTCTGTACC
TCTATGGCAGCCCGCGTGGGAACGGGTAACATGGCAGGTGTTGCAGTTGCATTAACTGTTGGCGGCCCTGGTGCTATCTT
CTGGATGTGGCTAATTGCTCTATTTGGTATGGCAACAGCTTTCATTGAATCAACACTAGCACAAGTTTACAAAGTAAAAG
ATGTTGATGGTCAATACCGTGGTGGCCCAGCTTATTACATGGAAAAAGGTTTAGGCCAACGCTGGATGGGTACGCTTTTC
TCTATCTTCTTAATCATCGCTTTTGGTCTAGTATTCAACGCTGTTCAAGCAAACACTATTACTGATGCTCTTAATCACTC
ATTTGGTTTTGATAAAACCATTATGGGTATCGTAATTGTTGTTATCTCTGGTTTCTTTATCATGGGTGGTTTACGTCGTG
TTGCTAACGCATCATCGAAAATCGTTCCTGTTATGGCGATTGGTTACTTAGCCATTGCTCTTATCATTGTAATAATGAAT
ATCACAGATGTACCGGCAGTATTGGTTCTTATCGTTAAAAGTGCATTTGGCTGGCAAGAAGCGGCTGCTGGTGGTGTGGC
GTACACTATTGCACAAGCTATGCAATCAGGTATCGCTCGTGGTCTATTCTCAAATGAAGCGGGTATGGGTTCTGCAGCTA
ACATCGCAGCAAGTGCTACGCCAAACCCTAACCACCCTGCATCACAAGGTTTTGTGCAAATGCTAGGTGTGTTTGTAGAT
ACATTAGTTATCTGTACAGCCTCTGCGGCGATGATTATGCTTTCTGGCGTAATGGATCAACCAGATGCAACGACAGGTAT
CAGCCTACTTCAACAAGCACTAACTAACGAGTTAGGCGGTTGGACAAGCTACTTTATGGCACTAGCTATTCTTCTGTTCT
GTTTCACATCTATCATTGCAAACTACAGCTACGCTGAAACAAACGTAATGTTCTTGAATGGTAATTCTAAGAAAGGCTTG
TTTGGCTTCCGTATGTGTGTGCTAGCAATGGTTATGTTTGGTTCAGTTGCGTCTCTGCCAGTAGTATGGAACCTTGCAGA
TGCTTCTATGGGTATGATGGCTTTAATCAACATTGTTGCTCTAGTTCTACTATCTAAACTAGCAATCAAAGTTATCAAAG
ATTACGAAGTTCAACTTAAAGCAGGAAAAACACCTGAATTTGACAGAACTAAGTTCCCTGAACTTGATGATTTAAAAGGT
GCGTGGCACCCAAAGAAAAAACAACACTAA

Upstream 100 bases:

>100_bases
ATACAACTTTATAACAAAGTTACTTATATTTCTTAAAGGATGGCGCTCGTTATCCATTATTTAAGAAAAATAAATTAACG
ATTAATAATAAGGAAATTTC

Downstream 100 bases:

>100_bases
TTTAATCCACAATTAGTTAAAAAAAGCCCGTCGAACTAAATTCGACGGGCTTTTTTATTACACCAATATACTGCATGAAT
CGTTAATCACGATTACCCCA

Product: sodium-linked D-alanine glycine permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTNTDSTLIESMNEVLLSIIGSINGLLWGQVLVYLLVGVGVYFTLRLGFIQIRQLGHSIKILRSGQEIENGISSYQVFCT
SMAARVGTGNMAGVAVALTVGGPGAIFWMWLIALFGMATAFIESTLAQVYKVKDVDGQYRGGPAYYMEKGLGQRWMGTLF
SIFLIIAFGLVFNAVQANTITDALNHSFGFDKTIMGIVIVVISGFFIMGGLRRVANASSKIVPVMAIGYLAIALIIVIMN
ITDVPAVLVLIVKSAFGWQEAAAGGVAYTIAQAMQSGIARGLFSNEAGMGSAANIAASATPNPNHPASQGFVQMLGVFVD
TLVICTASAAMIMLSGVMDQPDATTGISLLQQALTNELGGWTSYFMALAILLFCFTSIIANYSYAETNVMFLNGNSKKGL
FGFRMCVLAMVMFGSVASLPVVWNLADASMGMMALINIVALVLLSKLAIKVIKDYEVQLKAGKTPEFDRTKFPELDDLKG
AWHPKKKQH

Sequences:

>Translated_489_residues
MTNTDSTLIESMNEVLLSIIGSINGLLWGQVLVYLLVGVGVYFTLRLGFIQIRQLGHSIKILRSGQEIENGISSYQVFCT
SMAARVGTGNMAGVAVALTVGGPGAIFWMWLIALFGMATAFIESTLAQVYKVKDVDGQYRGGPAYYMEKGLGQRWMGTLF
SIFLIIAFGLVFNAVQANTITDALNHSFGFDKTIMGIVIVVISGFFIMGGLRRVANASSKIVPVMAIGYLAIALIIVIMN
ITDVPAVLVLIVKSAFGWQEAAAGGVAYTIAQAMQSGIARGLFSNEAGMGSAANIAASATPNPNHPASQGFVQMLGVFVD
TLVICTASAAMIMLSGVMDQPDATTGISLLQQALTNELGGWTSYFMALAILLFCFTSIIANYSYAETNVMFLNGNSKKGL
FGFRMCVLAMVMFGSVASLPVVWNLADASMGMMALINIVALVLLSKLAIKVIKDYEVQLKAGKTPEFDRTKFPELDDLKG
AWHPKKKQH
>Mature_488_residues
TNTDSTLIESMNEVLLSIIGSINGLLWGQVLVYLLVGVGVYFTLRLGFIQIRQLGHSIKILRSGQEIENGISSYQVFCTS
MAARVGTGNMAGVAVALTVGGPGAIFWMWLIALFGMATAFIESTLAQVYKVKDVDGQYRGGPAYYMEKGLGQRWMGTLFS
IFLIIAFGLVFNAVQANTITDALNHSFGFDKTIMGIVIVVISGFFIMGGLRRVANASSKIVPVMAIGYLAIALIIVIMNI
TDVPAVLVLIVKSAFGWQEAAAGGVAYTIAQAMQSGIARGLFSNEAGMGSAANIAASATPNPNHPASQGFVQMLGVFVDT
LVICTASAAMIMLSGVMDQPDATTGISLLQQALTNELGGWTSYFMALAILLFCFTSIIANYSYAETNVMFLNGNSKKGLF
GFRMCVLAMVMFGSVASLPVVWNLADASMGMMALINIVALVLLSKLAIKVIKDYEVQLKAGKTPEFDRTKFPELDDLKGA
WHPKKKQH

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=456, Percent_Identity=49.780701754386, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52388; Mature: 52256

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTDSTLIESMNEVLLSIIGSINGLLWGQVLVYLLVGVGVYFTLRLGFIQIRQLGHSIK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRSGQEIENGISSYQVFCTSMAARVGTGNMAGVAVALTVGGPGAIFWMWLIALFGMATA
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
FIESTLAQVYKVKDVDGQYRGGPAYYMEKGLGQRWMGTLFSIFLIIAFGLVFNAVQANTI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDALNHSFGFDKTIMGIVIVVISGFFIMGGLRRVANASSKIVPVMAIGYLAIALIIVIMN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
ITDVPAVLVLIVKSAFGWQEAAAGGVAYTIAQAMQSGIARGLFSNEAGMGSAANIAASAT
CCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEECCC
PNPNHPASQGFVQMLGVFVDTLVICTASAAMIMLSGVMDQPDATTGISLLQQALTNELGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
WTSYFMALAILLFCFTSIIANYSYAETNVMFLNGNSKKGLFGFRMCVLAMVMFGSVASLP
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHCCC
VVWNLADASMGMMALINIVALVLLSKLAIKVIKDYEVQLKAGKTPEFDRTKFPELDDLKG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCC
AWHPKKKQH
CCCCCCCCC
>Mature Secondary Structure 
TNTDSTLIESMNEVLLSIIGSINGLLWGQVLVYLLVGVGVYFTLRLGFIQIRQLGHSIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRSGQEIENGISSYQVFCTSMAARVGTGNMAGVAVALTVGGPGAIFWMWLIALFGMATA
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
FIESTLAQVYKVKDVDGQYRGGPAYYMEKGLGQRWMGTLFSIFLIIAFGLVFNAVQANTI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDALNHSFGFDKTIMGIVIVVISGFFIMGGLRRVANASSKIVPVMAIGYLAIALIIVIMN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
ITDVPAVLVLIVKSAFGWQEAAAGGVAYTIAQAMQSGIARGLFSNEAGMGSAANIAASAT
CCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEECCC
PNPNHPASQGFVQMLGVFVDTLVICTASAAMIMLSGVMDQPDATTGISLLQQALTNELGG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
WTSYFMALAILLFCFTSIIANYSYAETNVMFLNGNSKKGLFGFRMCVLAMVMFGSVASLP
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHCCC
VVWNLADASMGMMALINIVALVLLSKLAIKVIKDYEVQLKAGKTPEFDRTKFPELDDLKG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCC
AWHPKKKQH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]