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The map label for this gene is malE2 [H]

Identifier: 59712650

GI number: 59712650

Start: 2273248

End: 2274381

Strand: Direct

Name: malE2 [H]

Synonym: VF_2043

Alternate gene names: 59712650

Gene position: 2273248-2274381 (Clockwise)

Preceding gene: 59712649

Following gene: 59712651

Centisome position: 78.45

GC content: 42.42

Gene sequence:

>1134_bases
ATGATTAAGAAAATATCGCTTGCCGTATCAGCTGCACTACTCTTTTCAAGCACTGCAATGGCTGCAGATAATATCGTTAT
CGGCGTAGAAAAAAGCTATGTTCCTTACTTCACTGAATTAGCTGAGCAGTTCAACAAAGGTAAAGACTTTAAAGTAGAAG
TTGCTGCAACAAGTATGTTCGATTTACTGGCAGCACTACCAACGCAAAAAGGCAATATTGCTGATATTTTCATGATCCCA
AATGACCGTATCGGTGAGCTTGCGGACCAACATTTGATCACTCCAGCTAACTTTACTATCAATGGCTTTACTAAAGCAGC
GACGGATGCTGCAACGTATAACGGTCAAACGTACATGCTACCAATGTCGACAGATACGACTCTGTTCCTTTACAACAAAG
ACATGGTGACTGAAGTACCAAAAACATTAAAAGAAATTCCGCCATCAGAATGGGCTGCTAAGTTCACAGACTTCTACTTC
ACTGGTGGTCTATTCATGACTAACGGTGGTTACATTTTCCAAGACAATAACCCTGAAAATATTGGTCTGAACAACGAAGG
CTCAATTAAAGCTGGCCTTGCAGCTCAAAGCCTTTACAAGAGTGGCGTAGGCCACTGGACACTAATGCAAGATGACACGG
TTGCTTATGACATCATGATGAAATACTTCATGGAAGGTAAGCTAAAAGCCATCATCAATGGTCCTTGGGCAATTGCTGAT
ATTGAAAAATCAGGGGTAAACGTTGGTGCGGCTCCAATTCCTAGCTGGGATGGCAGTCATCCATACAAAGCCCTTACTGG
CACAAAAGGCATGACCATTAACGGTTACAGCAGCAACAAAGAAGGTGCAAATGCCTTCATTAAGTTTATGGCAACACCAG
AAAATGCACAAAAATGGTACTCAATGACTCGTGAAGTATCACCTAGCCTTTCTGTTACGTACGAAGAAGGCTCACTGCAT
AAAGCCATTTTCGATGCAACGAACATTGGTCAACCTATGCCAAGCATTCCTGAGTTCATGAAAGTATGGGGCCCGATGAA
AACGGCTCTAGCTCAAATCGCTCAAGGTCAAGATACCAAAGCAGTACTTGATGCCGCAGTTGAGACAATCGAAATCGACA
TTGAAGATATGTAA

Upstream 100 bases:

>100_bases
GATTAAATCAAAGGTAACTCATTGATATAACAATAAAGCTACCTTTACTCACGTAATTTTATTGATTCAAAATACTTTAT
CCCTTTTCAGGAGAAATCAA

Downstream 100 bases:

>100_bases
TTCACTCTTTTCAACGTACCAACCTCAACGGTGTCCCTATTAAATTAGGGCATCGTTGTCCTCTAAATCCGTTACATATC
TACACTAACTTAATAAAAAA

Product: maltose/maltodextrin-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIKKISLAVSAALLFSSTAMAADNIVIGVEKSYVPYFTELAEQFNKGKDFKVEVAATSMFDLLAALPTQKGNIADIFMIP
NDRIGELADQHLITPANFTINGFTKAATDAATYNGQTYMLPMSTDTTLFLYNKDMVTEVPKTLKEIPPSEWAAKFTDFYF
TGGLFMTNGGYIFQDNNPENIGLNNEGSIKAGLAAQSLYKSGVGHWTLMQDDTVAYDIMMKYFMEGKLKAIINGPWAIAD
IEKSGVNVGAAPIPSWDGSHPYKALTGTKGMTINGYSSNKEGANAFIKFMATPENAQKWYSMTREVSPSLSVTYEEGSLH
KAIFDATNIGQPMPSIPEFMKVWGPMKTALAQIAQGQDTKAVLDAAVETIEIDIEDM

Sequences:

>Translated_377_residues
MIKKISLAVSAALLFSSTAMAADNIVIGVEKSYVPYFTELAEQFNKGKDFKVEVAATSMFDLLAALPTQKGNIADIFMIP
NDRIGELADQHLITPANFTINGFTKAATDAATYNGQTYMLPMSTDTTLFLYNKDMVTEVPKTLKEIPPSEWAAKFTDFYF
TGGLFMTNGGYIFQDNNPENIGLNNEGSIKAGLAAQSLYKSGVGHWTLMQDDTVAYDIMMKYFMEGKLKAIINGPWAIAD
IEKSGVNVGAAPIPSWDGSHPYKALTGTKGMTINGYSSNKEGANAFIKFMATPENAQKWYSMTREVSPSLSVTYEEGSLH
KAIFDATNIGQPMPSIPEFMKVWGPMKTALAQIAQGQDTKAVLDAAVETIEIDIEDM
>Mature_377_residues
MIKKISLAVSAALLFSSTAMAADNIVIGVEKSYVPYFTELAEQFNKGKDFKVEVAATSMFDLLAALPTQKGNIADIFMIP
NDRIGELADQHLITPANFTINGFTKAATDAATYNGQTYMLPMSTDTTLFLYNKDMVTEVPKTLKEIPPSEWAAKFTDFYF
TGGLFMTNGGYIFQDNNPENIGLNNEGSIKAGLAAQSLYKSGVGHWTLMQDDTVAYDIMMKYFMEGKLKAIINGPWAIAD
IEKSGVNVGAAPIPSWDGSHPYKALTGTKGMTINGYSSNKEGANAFIKFMATPENAQKWYSMTREVSPSLSVTYEEGSLH
KAIFDATNIGQPMPSIPEFMKVWGPMKTALAQIAQGQDTKAVLDAAVETIEIDIEDM

Specific function: Part of the ABC transporter complex involved in maltodextrin import. Binds maltodextrin. Can also bind maltose with low affinity, but is not involved in its uptake [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=383, Percent_Identity=28.9817232375979, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41152; Mature: 41152

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKISLAVSAALLFSSTAMAADNIVIGVEKSYVPYFTELAEQFNKGKDFKVEVAATSMF
CCEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHHHHH
DLLAALPTQKGNIADIFMIPNDRIGELADQHLITPANFTINGFTKAATDAATYNGQTYML
HHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEECCCHHHCCCCEEECCEEEEE
PMSTDTTLFLYNKDMVTEVPKTLKEIPPSEWAAKFTDFYFTGGLFMTNGGYIFQDNNPEN
EECCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHEEEECCEEEECCEEEEECCCCCC
IGLNNEGSIKAGLAAQSLYKSGVGHWTLMQDDTVAYDIMMKYFMEGKLKAIINGPWAIAD
CCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEE
IEKSGVNVGAAPIPSWDGSHPYKALTGTKGMTINGYSSNKEGANAFIKFMATPENAQKWY
ECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCEEEEEEECCCHHHHHH
SMTREVSPSLSVTYEEGSLHKAIFDATNIGQPMPSIPEFMKVWGPMKTALAQIAQGQDTK
HHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCHH
AVLDAAVETIEIDIEDM
HHHHHHHEEEEEEEECC
>Mature Secondary Structure
MIKKISLAVSAALLFSSTAMAADNIVIGVEKSYVPYFTELAEQFNKGKDFKVEVAATSMF
CCEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHHHHH
DLLAALPTQKGNIADIFMIPNDRIGELADQHLITPANFTINGFTKAATDAATYNGQTYML
HHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEECCCHHHCCCCEEECCEEEEE
PMSTDTTLFLYNKDMVTEVPKTLKEIPPSEWAAKFTDFYFTGGLFMTNGGYIFQDNNPEN
EECCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHEEEECCEEEECCEEEEECCCCCC
IGLNNEGSIKAGLAAQSLYKSGVGHWTLMQDDTVAYDIMMKYFMEGKLKAIINGPWAIAD
CCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCEEEEE
IEKSGVNVGAAPIPSWDGSHPYKALTGTKGMTINGYSSNKEGANAFIKFMATPENAQKWY
ECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCEEEEEEECCCHHHHHH
SMTREVSPSLSVTYEEGSLHKAIFDATNIGQPMPSIPEFMKVWGPMKTALAQIAQGQDTK
HHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHCCCCHH
AVLDAAVETIEIDIEDM
HHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]