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The map label for this gene is pyrF [H]

Identifier: 59712362

GI number: 59712362

Start: 1982146

End: 1982850

Strand: Reverse

Name: pyrF [H]

Synonym: VF_1755

Alternate gene names: 59712362

Gene position: 1982850-1982146 (Counterclockwise)

Preceding gene: 59712363

Following gene: 59712360

Centisome position: 68.43

GC content: 41.13

Gene sequence:

>705_bases
ATGAAAGACCAAAAGGTGATCGTGGCACTGGATTATGATAATCAAGCAGATGCGTTAACGTTTGTTGATAAAATTGATCC
AAGTTCATGTCGTTTAAAAGTAGGCAAAGAGATGTTTACTTTATTTGGGCCTGAGTTTGTTAAAGAACTTCATAAACGTG
GTTTTTCTGTTTTTCTAGATTTAAAGTTTCATGATATTCCAAATACCTGTTCAAAAGCGGTAAGAGCGGCAGCAGAAATG
GGTGTATGGATGGTTAACGTTCATGCGAGTGGTGGTGAGCGTATGATGAGTGCCTCGCGTGAAATATTAGAACCTTATGG
TAGTGACCGTCCATTATTAATTGGTGTGACGGTGTTAACGAGCATGGAGCAGCAAGACTTAGCTGGCATTGGTCTAGACA
TAGCACCACAAGAACAAGTAAAACGTTTAGCGGCATTAACGAAAAACAGCGGTTTAGATGGTGTCGTATGTTCAGCTCAA
GAGGCATCAATGCTAAAAGCTGATTTAGGTAAAGAGTTCCAGTTAGTAACACCAGGAATTCGTCCAGCAGGTGCTGATGT
TGGTGACCAAAAGCGTATTATGACGCCAGTAGATGCGATTACAGCAGGTTCTGACTACCTAGTTATAGGTCGCCCGATTA
CACAAGCGGAGAATCCATCACAGGTCCTTAATGAGATTAATCTTTCTCTCGCTTCTGTTTTATAA

Upstream 100 bases:

>100_bases
CTCTGAGTGCCTCTTTTTTTGAATGTAAGAAAAGACCGTGAGTACGACTAATCCTCATGATGAATTGATGGGATTGGTAT
AAATTTTTAAAGGAGAGAAA

Downstream 100 bases:

>100_bases
CGGGTAATATTATTAAAACATAAAAAAATAGAGACCATTTGGTCTCTATTTTTGTTTGTAAGTTATACCAATCACAGTAA
GTAATTGATTTACTACGACT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKDQKVIVALDYDNQADALTFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEM
GVWMVNVHASGGERMMSASREILEPYGSDRPLLIGVTVLTSMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQ
EASMLKADLGKEFQLVTPGIRPAGADVGDQKRIMTPVDAITAGSDYLVIGRPITQAENPSQVLNEINLSLASVL

Sequences:

>Translated_234_residues
MKDQKVIVALDYDNQADALTFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEM
GVWMVNVHASGGERMMSASREILEPYGSDRPLLIGVTVLTSMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQ
EASMLKADLGKEFQLVTPGIRPAGADVGDQKRIMTPVDAITAGSDYLVIGRPITQAENPSQVLNEINLSLASVL
>Mature_234_residues
MKDQKVIVALDYDNQADALTFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEM
GVWMVNVHASGGERMMSASREILEPYGSDRPLLIGVTVLTSMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQ
EASMLKADLGKEFQLVTPGIRPAGADVGDQKRIMTPVDAITAGSDYLVIGRPITQAENPSQVLNEINLSLASVL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=67.5555555555556, Blast_Score=318, Evalue=1e-88,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25304; Mature: 25304

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQKVIVALDYDNQADALTFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLD
CCCCEEEEEEECCCCCCCEEHEECCCCCHHEEHHHHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVRAAAEMGVWMVNVHASGGERMMSASREILEPYGSDRPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHH
SMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQEASMLKADLGKEFQLVTPGI
CCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCEEEECCCC
RPAGADVGDQKRIMTPVDAITAGSDYLVIGRPITQAENPSQVLNEINLSLASVL
CCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDQKVIVALDYDNQADALTFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLD
CCCCEEEEEEECCCCCCCEEHEECCCCCHHEEHHHHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVRAAAEMGVWMVNVHASGGERMMSASREILEPYGSDRPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHH
SMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQEASMLKADLGKEFQLVTPGI
CCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHCCCCEEEECCCC
RPAGADVGDQKRIMTPVDAITAGSDYLVIGRPITQAENPSQVLNEINLSLASVL
CCCCCCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA